Improving olefin tolerance and production in E. coli using native and evolved AcrB

Biotechnology and Bioengineering

Volumn 112, Issue 5, 2015, Pages 879-888

  Mingardon, Florence ^a   Clement, Camille ^a   Hirano, Kathleen ^b,c   Nhan, Melissa ^b,c   Luning, Eric G ^c   Chanal, Angelique ^a   Mukhopadhyay, Aindrila ^b,c  

^a Total Gas and Power New Energies USA Inc   (United States)

^b JOINT BIOENERGY INSTITUTE   (United States)

^c LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

Directed evolution; Host engineering; Olefin production; Solvent tolerance

Indexed keywords

ESCHERICHIA COLI; OPTIMIZATION; STYRENE;

DIRECTED EVOLUTION; LIMIT PRODUCTIONS; NEGATIVE SELECTION; OLEFIN PRODUCTION; OPTIMAL PRODUCTION; POLYMER INDUSTRIES; SOLVENT TOLERANCE; SYNERGISTIC MANNER;

OLEFINS;

1 HEXENE; 1 NONENE; 1 OCTENE; ALKENE DERIVATIVE; STYRENE; UNCLASSIFIED DRUG; 1-HEXENE; 1-OCTENE; ACRB PROTEIN, E COLI; ALKENE; ESCHERICHIA COLI PROTEIN; MULTIDRUG RESISTANCE PROTEIN;

ARTICLE; CONTROLLED STUDY; DRUG MANUFACTURE; DRUG POTENCY; DRUG TOLERABILITY; DRUG TOLERANCE; ESCHERICHIA COLI; INNER MEMBRANE; MUTAGENESIS; MUTATION; NONHUMAN; PHENOTYPE; CHEMICAL STRUCTURE; CHEMISTRY; DRUG EFFECTS; GENETICS; METABOLISM; MICROBIOLOGY; POINT MUTATION; PROTEIN ENGINEERING; PROTEIN TERTIARY STRUCTURE;

ESCHERICHIA COLI;

ALKENES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; INDUSTRIAL MICROBIOLOGY; MODELS, MOLECULAR; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; POINT MUTATION; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY;

EID: 84925637685     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25511     Document Type: Article

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