Mineralization initiation of MC3T3-E1 preosteoblast is suppressed under simulated microgravity condition

Cell Biology International

Volumn 39, Issue 4, 2015, Pages 364-372

  Hu, Li fang ^a   Li, Jing bao ^a   Qian, Ai rong ^a   Wang, Fei ^a   Shang, Peng ^a  

^a NORTHWESTERN POLYTECHNICAL UNIVERSITY   (China)

Author keywords

Bone loss; ERK activity; Osteoblast mineralization; Osteogenic gene; Random positioning machine; Simulated microgravity

Indexed keywords

ALKALINE PHOSPHATASE; COLLAGEN TYPE 1; COLLAGEN TYPE 1 ALPHA 1; DENTIN MATRIX PROTEIN 1; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; OSTEOCALCIN; TRANSCRIPTION FACTOR RUNX2; UNCLASSIFIED DRUG; COLLAGEN TYPE I, ALPHA 1 CHAIN; MINERAL;

ALP GENE; ANIMAL CELL; ARTICLE; BONE MINERALIZATION; CELL DIFFERENTIATION; CELL MATURATION; COL I ALPHA 1 GENE; COMPARATIVE STUDY; CONTROLLED STUDY; DMP1 GENE; DOWN REGULATION; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; GENE EXPRESSION REGULATION; INTRACELLULAR SIGNALING; LABORATORY DEVICE; MC3T3 E1 PREOSTEOBLAST; MICROGRAVITY; MOLECULAR DYNAMICS; MOUSE; NONHUMAN; OC GENE; OSTEOBLAST; PROTEIN EXPRESSION; RANDOM POSITIONING MACHINE; SIMULATED MICROGRAVITY; WESTERN BLOTTING; ANIMAL; BONE DEVELOPMENT; CELL LINE; CYTOLOGY; GENETICS; METABOLISM; PHOSPHORYLATION; SIGNAL TRANSDUCTION; WEIGHTLESSNESS;

MURINAE;

ALKALINE PHOSPHATASE; ANIMALS; CELL DIFFERENTIATION; CELL LINE; COLLAGEN TYPE I; CORE BINDING FACTOR ALPHA 1 SUBUNIT; DOWN-REGULATION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; MICE; MINERALS; OSTEOBLASTS; OSTEOCALCIN; OSTEOGENESIS; PHOSPHORYLATION; SIGNAL TRANSDUCTION; WEIGHTLESSNESS SIMULATION;

EID: 84925250098     PISSN: 10656995     EISSN: 10958355     Source Type: Journal    
DOI: 10.1002/cbin.10391     Document Type: Article

References (64)

1. Anderson HC ( 1995) Molecular biology of matrix vesicles. Clin Orthop Relat Res 314: 266-80.
2. Balcerzak M, Hamade E, Zhang L, Pikula S, Azzar G, Radisson J, Bandorowicz-Pikula J, Buchet R ( 2003) The roles of annexins and alkaline phosphatase in mineralization process. Acta Biochim Pol 50: 1019-38.
3. Boskey AL ( 1996) Matrix proteins and mineralization: an overview. Connect Tissue Res 35: 357-63.
4. Boskey AL ( 1998) Biomineralization: conflicts, challenges, and opportunities. J Cell Biochem 30-31: 83-91.
5. Candeliere GA, Liu F, Aubin JE ( 2001) Individual osteoblasts in the developing calvaria express different gene repertoires. Bone 28: 351-61.
6. Cao YJ, Fan XJ, Shen Z, Ma BH, Duan EK ( 2007) Nitric oxide affects preimplantation embryonic development in a rotating wall vessel bioreactor simulating microgravity. Cell Biol Int 31: 24-9.
7. Carmeliet G, Nys G, Bouillon R ( 1997) Microgravity reduces the differentiation of human osteoblastic MG-63 cells. J Bone Miner Res 12: 786-94.
8. Carmeliet G, Vico L, Bouillon R ( 2001) Space flight: a challenge for normal bone homeostasis. Crit Rev Eukaryot Gene Expr 11: 131-44.
9. Collet P, Uebelhart D, Vico L, Moro L, Hartmann D, Roth M, Alexandre C ( 1997) Effects of 1- and 6-month spaceflight on bone mass and biochemistry in two humans. Bone 20: 547-51.
10. Cowles EA, DeRome ME, Pastizzo G, Brailey LL, Gronowicz GA ( 1998) Mineralization and the expression of matrix proteins during in vivo bone development. Calcif Tissue Int 62: 74-82.
11. Ecarot-Charrier B, Glorieux FH, van der Rest M, Pereira G ( 1983) Osteoblasts isolated from mouse calvaria initiate matrix mineralization in culture. J Cell Biol 96: 639-43.
12. Fen JQ, Zhang J, Dallas SL, Lu Y, Chen S, Tan X, Owen M, Harris SE, MacDougall M ( 2002) Dentin matrix protein 1, a target molecule for Cbfa1 in bone, is a unique bone marker gene. J Bone Miner Res 17: 1822-31.
13. Ge C, Xiao G, Jiang D, Franceschi RT ( 2007) Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development. J Cell Biol 176: 709-18.
14. Ge C, Xiao G, Jiang D, Yang Q, Hatch NE, Roca H, Franceschi RT ( 2009) Identification and functional characterization of ERK/MAPK phosphorylation sites in the Runx2 transcription factor. J Biol Chem 284: 32533-43.
15. Gershovich PM, Gershovich JG, Zhambalova AP, Romanov YA, Buravkova LB ( 2012) Cytoskeletal proteins and stem cell markers gene expression in human bone marrow mesenchymal stromal cells after different periods of simulated microgravity. Acta Astronaut 70: 36-42.
16. Harris H ( 1990) The human alkaline phosphatases: what we know and what we don't know. Clin Chim Acta 186: 133-50.
17. Hauschka PV, Lian JB, Cole DE, Gundberg CM ( 1989) Osteocalcin and matrix Gla protein: vitamin K-dependent proteins in bone. Physiol Rev 69: 990-1047.
18. Higuchi C, Myoui A, Hashimoto N, Kuriyama K, Yoshioka K, Yoshikawa H, Itoh K ( 2002) Continuous inhibition of MAPK signaling promotes the early osteoblastic differentiation and mineralization of the extracellular matrix. J Bone Miner Res 17: 1785-94.
19. Hu LF, Qian AR, Wang Y, Di SM Shang ( 2013) Inhibitory effect of simulated microgravity on differentiating preosteoblasts. Adv Space Res 51: 107-14.
20. Hughes-Fulford M ( 2002) Physiological effects of microgravity on osteoblast morphology and cell biology. Adv Space Biol Med 8: 129-57.
21. Hughes-Fulford M, Lewis ML ( 1996) Effects of microgravity on osteoblast growth activation. Exp Cell Res 224: 103-09.
22. Keyak JH, Koyama AK, LeBlanc A, Lu Y, Lang TF ( 2009) Reduction in proximal femoral strength due to long-duration spaceflight. Bone 44: 449-53.
23. Kong WX, Zhu H, Jiang XX, Li H, Liu YL, Wu Y, Zhang Y, Mao N ( 2010) Involvement of MAPK pathway in the osteoblastic differentiation of mouse mesenchymal stem cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi 18: 981-85.
24. Kono S-j, Oshima Y, Hoshi K, Bonewald LF, Oda H, Nakamura K, Kawaguchi H, Tanaka S ( 2007) Erk pathways negatively regulate matrix mineralization. Bone 40: 68-74.
25. Lang T, LeBlanc A, Evans H, Lu Y, Genant H, Yu A ( 2004) Cortical and trabecular bone mineral loss from the spine and hip in long-duration spaceflight. J Bone Miner Res 19: 1006-12.
26. LeBlanc AD, Spector ER, Evans HJ, Sibonga JD ( 2007) Skeletal responses to space flight and the bed rest analog: a review. J Musculoskelet Neuronal Interact 7: 33-47.
27. Lian J, Stein GS, Canalis E ( 1999) Bone formation: osteoblast lineage cells, growth factors, matrix proteins, and the mineralization process. Primer on the metabolic bone diseases and disorders of mineral metabolism. In: Favus MJ eds. Philadelphia: Lippincott Williams & Wilkins, pp. 14-29.
28. Ling Y, Rios HF, Myers ER, Lu Y, Feng JQ, Boskey AL ( 2005) DMP1 depletion decreases bone mineralization in vivo: an FTIR imaging analysis. J Bone Miner Res 20: 2169-77.
29. Livak KJ, Schmittgen TD ( 2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT Method. Method 25: 402-08.
30. Luo MZ, Yang ZQ, Li JB, Xu HY, Li SS, Zhang W, Qian AR, Shang P ( 2013) Calcium influx through stretch-activated channels mediates microfilament reorganization in osteoblasts under simulated weightlessness. Adv Space Res 51: 2058-68.
31. Makihira S, Kawahara Y, Yuge L, Mine Y, Nikawa H ( 2008) Impact of the microgravity environment in a 3-dimensional clinostat on osteoblast- and osteoclast-like cells. Cell Biol Int 32: 1176-81.
32. Marie PJ, Jones D, Vico L, Zallone A, Hinsenkamp M, Cancedda R ( 2000) Osteobiology, strain, and microgravity: part I. Studies at the cellular level 67: 2-9.
33. Matsushita T, Chan YY, Kawanami A, Balmes G, Landreth GE, Murakami S ( 2009) Extracellular signal-regulated kinase 1 (ERK1) and ERK2 play essential roles in osteoblast differentiation and in supporting osteoclastogenesis. Mol Cell Biol 29: 5843-57.
34. Morey E, Baylink D ( 1978) Inhibition of bone formation during space flight. Science 201: 1138-41.
35. Nadia Rucci ( 2009) Anna Rufo. Anna Teti 19: 139-49.
36. Nakamura H ( 2007) Morphology, function, and differentiation of bone cells. J Hard Tissue Biol 16: 15-22.
37. Nakayama K, Tamura Y, Suzawa M, Harada S, Fukumoto S, Kato M, Miyazono K, Rodan GA, Takeuchi Y, Fujita T ( 2003) Receptor tyrosine kinases inhibit bone morphogenetic protein-Smad responsive promoter activity and differentiation of murine MC3T3-E1 osteoblast-like cells. J Bone Miner Res 18: 827-35.
38. Narayanan K, Srinivas R, Ramachandran A, Hao J, Quinn B, George A ( 2001) Differentiation of embryonic mesenchymal cells to odontoblast-like cells by overexpression of dentin matrix protein 1. Proc Natl Acad Sci U S A 98: 4516-21.
39. Orimo H ( 2010) The mechanism of mineralization and the role of alkaline phosphatase in health and disease. J Nippon Med Sch 77: 4-12.
40. Orimo H, Shimada T ( 2008) The role of tissue-nonspecific alkaline phosphatase in the phosphate-induced activation of alkaline phosphatase and mineralization in SaOS-2 human osteoblast-like cells. Mol Cell Biochem 315: 51-60.
41. Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS ( 1990) Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143: 420-30.
42. Papachristou DJ, Papachroni KK, Basdra EK, Papavassiliou AG ( 2009) Signaling networks and transcription factors regulating mechanotransduction in bone. Bioessays 31: 794-80.
43. Pardo SJ, Patel MJ, Sykes MC, Platt MO, Boyd NL, Sorescu GP, Xu M, van Loon JJ, Wang MD, Jo H ( 2005) Simulated microgravity using the Random Positioning Machine inhibits differentiation and alters gene expression profiles of 2T3 preosteoblasts. Am J Physiol Cell Physiol 288: C1211-21.
44. Patel MJ, Chang KH, Sykes MC, Talish R, Rubin C, Jo H ( 2009) Low magnitude and high frequency mechanical loading prevents decreased bone formation responses of 2T3 preosteoblasts. J Cell Biochem 106: 306-16.
45. Qian AR, Yin DC, Yang PF, Lv Y, Tian ZC, Shang P ( 2013) Application of diamagnetic levitation technology in biological sciences research. IEEE T Appl Supercon 23: -3600305.
46. Rizzo AM, Montorfano G, Negroni M, Corsetto P, Berselli P, Marciani P, Zava S, Berra B ( 2009) Simulated microgravity induce glutathione antioxidant pathway in Xenopus laevis embryos. Cell Biol Int 33: 893-98.
47. Rucci N, Migliaccio S, Zani BM, Taranta A, Teti A ( 2002) Characterization of the osteoblast-like cell phenotype under microgravity conditions in the NASA-approved Rotating Wall Vessel bioreactor (RWV). J Cell Biochem 85: 167-79.
48. Saxena R, Pan G, Dohm E, McDonald J ( 2011) Modeled microgravity and hindlimb unloading sensitize osteoclast precursors to RANKL-mediated osteoclastogenesis. J Bone Miner Metab 29: 111-22.
49. Sun Y, Shuang F, Chen DM, Zhou RB ( 2013) Treatment of hydrogen molecule abates oxidative stress and alleviates bone loss induced by modeled microgravity in rats. Osteoporos Int 24: 969-78.
50. Tenenbaum HC ( 1981) Role of organic phosphate in mineralization of bone in vitro. J Dent Res;60 Spec No C: 1: 1586-89.
51. Tenenbaum HC ( 1987) Levamisole and inorganic pyrophosphate inhibit beta-glycerophosphate induced mineralization of bone formed in vitro. Bone Miner 3: 13-26.
52. Turner RT, Evans GL, Wakley GK ( 1995) Spaceflight results in depressed cancellous bone formation in rat humeri. Aviat Space Environ Med 66: 770-74.
53. van Loon JJ ( 2007) Some history and use of the random positioning machine. RPM 39: 1161-65.
54. van Loon JJ, Bervoets DJ, Burger EH, Dieudonne SC, Hagen JW, Semeins CM, Doulabi BZ, Veldhuijzen JP ( 1995) Decreased mineralization and increased calcium release in isolated fetal mouse long bones under near weightlessness. J Bone Miner Res 10: 550-57.
55. Vico L, Collet P, Guignandon A, Lafage-Proust MH, Thomas T, Rehaillia M, Alexandre C ( 2000) Effects of long-term microgravity exposure on cancellous and cortical weight-bearing bones of cosmonauts. Lancet 355: 1607-11.
56. Vico L, Hinsenkamp M, Jones D, Marie PJ, Zallone A, Cancedda R ( 2001) Osteobiology, strain, and microgravity. Part II: studies at the tissue level. Calcif Tissue Int 68: 1-10.
57. Wang X, Harimoto K, Liu J, Guo J, Hinshaw S, Chang Z, Wang Z ( 2011) Spata4 promotes osteoblast differentiation through Erk-activated Runx2 pathway. J Bone Miner Res 26: 1964-73.
58. Wennberg C, Hessle L, Lundberg P, Mauro S, Narisawa S, Lerner UH, Millan JL ( 2000) Functional characterization of osteoblasts and osteoclasts from alkaline phosphatase knockout mice. J Bone Miner Res 15: 1879-88.
59. Wronski TJ, Morey ER ( 1983) Effect of spaceflight on periosteal bone formation in rats. Am J Physiol 244: R305-09.
60. Xiao G, Gopalakrishnan R, Jiang D, Reith E, Benson MD, Franceschi RT ( 2002) Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells. J Bone Miner Res 17: 101-10.
61. Xiao G, Jiang D, Thomas P, Benson M, Guan K, Karsenty G, Franceschi R ( 2000) MAPK pathways activate and phosphorylate the osteoblast-specific transcription factor. Cbfa1 J Biol Chem 275: 4453-59.
62. Yuge L, Hide I, Kumagai T, Kumei Y, Takeda S, Kanno M, Sugiyama M, Kataoka K ( 2003) Cell differentiation and p38(MAPK) cascade are inhibited in human osteoblasts cultured in a three-dimensional clinostat. In Vitro Cell Dev Biol Anim 39: 89-97.
63. Zerath E, Holy X, Roberts SG, Andre C, Renault S, Hott M, Marie PJ ( 2000) Spaceflight inhibits bone formation independent of corticosteroid status in growing rats. J Bone Miner Res 15: 1310-20.
64. Zerath E, Novikov V, Leblanc A, Bakulin A, Oganov V, Grynpas M ( 1996) Effects of spaceflight on bone mineralization in the rhesus monkey. J Appl Physiol 81: 194-200.