Characterization of the osteoblast-like cell phenotype under microgravity conditions in the NASA-approved rotating wall vessel bioreactor (RWV)

Journal of Cellular Biochemistry

Volumn 85, Issue 1, 2002, Pages 167-179

  Rucci, Nadia ^a,c   Migliaccio, Silvia ^a   Zani, Bianca Maria ^a   Taranta, Anna ^a   Teti, Anna ^b  

^a UNIVERSITY OF L'AQUILA   (Italy)

^b LABORATORIO DI PATOLOGIA VASCOLARE   (Italy)

^c Department of Experimental Medicine ^*  (Italy)

Author keywords

Apoptosis; Bone remodelling; Microgravity; Osteoblast

Indexed keywords

ALKALINE PHOSPHATASE; BISBENZIMIDE; BONE MORPHOGENETIC PROTEIN 2; BONE MORPHOGENETIC PROTEIN 4; CASPASE 8; DNA FRAGMENT; INTERLEUKIN 6; MESSENGER RNA; OSTEOCALCIN; OSTEONECTIN; OSTEOPONTIN; PROTEIN BAX; PROTEIN BCL 2; PROTEIN P53; SIALOPROTEIN;

ANIMAL CELL; APOPTOSIS; ARTICLE; BIOREACTOR; BONE ATROPHY; CELL ACTIVITY; CELL CULTURE; CELL LEVEL; CELL LINE; CELL PROLIFERATION; CELL STIMULATION; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; ELECTROPHORESIS; ENZYME ACTIVITY; MICROGRAVITY; NONHUMAN; NUCLEOSOME; NUCLEOTIDE SEQUENCE; OSTEOBLAST; OSTEOCLAST; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; ROTATING WALL VESSEL BIOREACTOR; SIGNAL TRANSDUCTION; WEIGHTLESSNESS;

ALKALINE PHOSPHATASE; ANIMALS; APOPTOSIS; BIOREACTORS; BONE MORPHOGENETIC PROTEINS; CELL DIVISION; CELL SURVIVAL; CELLS, CULTURED; CYTOKINES; CYTOSKELETON; OSTEOBLASTS; OSTEOCALCIN; OSTEONECTIN; PHENOTYPE; RATS; RNA, MESSENGER; SIALOGLYCOPROTEINS; TRANSFORMING GROWTH FACTOR BETA; UNITED STATES; UNITED STATES NATIONAL AERONAUTICS AND SPACE ADMINISTRATION; WEIGHTLESSNESS;

ANIMALIA;

EID: 0036194567     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcb.10120     Document Type: Article

References (47)

1. Pulsed electromagnetic fields simultaneously induce osteogenesis and upregulate transcription of bone morphogenetic proteins 2 and 4 in rat osteoblasts in vitro
2. The ligand osteoprotegerin (OPGL) directly activates mature osteoclasts
3. Space flight is associated with rapid decreases of undercarboxylated osteocalcin and increases of markers of bone resorption without changes in their circadian variation: Observations in two cosmonauts
4. The effect of microgravity on morphology and gene expression of osteoblasts in vitro
5. The effects of orbital spaceflight on bone histomorphometry and messenger ribonucleic acid levels for bone matrix proteins and skeletal signaling peptides in ovariectomized growing rats
6. Single step method of RNA isolation by guanidinium thiocyanate-phenol-chloroform extraction
7. Tissue culture in microgravity
8. Antisera and cDNA probes to human and certain animal model bone matrix proteins
9. Tissue engineering of cartilage in space
10. Microgravity cultivation of cells and tissues
11. Perspectives: Some roles of mechanical usage, muscle strength, and mechanostat in skeletal physiology, disease, and research
12. The temporal response of bone to unloading
13. Rotating wall vessels, promising bioreactors for osteoblastic cell culture. Comparison with other 3D conditions
14. Apoptotic pathways: Papers wraps stone blunts scissors
15. Effects of orbital spaceflight on human osteoblastic cell physiology and gene expression
16. In vitro maintenance of normal and pathological human salivary gland tissue in a NASA-designed rotating wall vessel bioreactor
17. The biochemistry of apoptosis
18. NASA biotechnology: Cell science in microgravity
19. Effects of simulated microgravity on human osteoblast-like cells in culture
20. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation
21. Spaceflight effects on cultured embryonic chick bone cells
22. Use of microgravity bioreactors for development of an in vitro salivary gland cell culture model
23. Bone formation: Osteoblast lineage cells, growth factors, matrix proteins, and the mineralization process
24. Parathyroid hormone (PTH) and PTH-like protein (PLP) stimulate interleukin-6 production by osteogenic cells: A possible role of interleukin-6 in osteoclastogenesis
25. Role of cytokines in bone resorption
26. The role of IL-6 type cytokines and their receptors in bone
27. Birth and death of bone cells: Basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis
28. Osteobiology, strain, and microgravity: Part I. Studies at the cellular level
29. Estrogens modulate the responsiveness of osteoblast-like cells (ROS 17/2.8) stably transfected with estrogen receptor
30. Inhibition of bone formation during space flight
31. Novel approaches to cellular transplantation from the U.S. Space Program
32. Melanoma cells stimulate osteoclastogenesis, c-src expression and osteoblast cytokines
33. Forearm bone loss in hemiplagia: A model for the study of immobilization osteoporosis
34. Fabrication, characterization and evaluation of bioceramic hollow microspheres used as microcarriers for 3-D bone tissue formation in rotating bioreactors
35. 3D bone tissue engineered with bioactive microspheres in simulated microgravity
36. Control of bone formation and resorption: Biological and clinical perspective
37. Regulation of Bcl-xL expression in human keratinocytes by cell-substratum adhesion and the epidermal growth factor receptor
38. Culture in vector-averaged gravity under clinostat rotation results in apoptosis of osteoblastic ROS 17/2.8 cells
39. Cell culture for three-dimensional modeling in rotating wall vessels: An application of simulated microgravity
40. Effect of space-flight and simulated weightlessness on longitudinal bone growth
41. Physiological properties and differential glycosylation of phosphorylated and nonphosphorylated forms of osteopontin secreted by normal rat kidney cells
42. Physiology of a microgravity environment. Invited review: What do we know about the effects of spaceflight on bone?
43. Decreased mineralization and increased calcium release in isolated fetal mouse long bones under near weightlessness
44. Effect of weightlessness on bone mass and osteoclast number in pregnant rats after a five-day spaceflight (COSMOS 1514)
45. Effect of long-term microgravity exposure on cancellous and cortical weightbearing bones of cosmonauts
46. Physiology in microgravity
47. Establishment of three-dimensional human prostate organoid cocolture under microgravity-simulated conditions: Evaluation of androgen-induced growth and PSA expression