Physiological effects of microgravity on osteoblast morphology and cell biology

Advances in Space Biology and Medicine

Volumn 8, Issue , 2002, Pages 129-157

  Hughes Fulford, Millie ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; CELL DIFFERENTIATION; CELL DIVISION; CYTOLOGY; CYTOSKELETON; FLIGHT EXPERIMENT; FOTON PROJECT; GENE EXPRESSION; HUMAN; MANNED; MOLECULAR BIOLOGY; NASA DISCIPLINE CELL BIOLOGY; NASA PROGRAM FUNDAMENTAL SPACE BIOLOGY; NON-NASA CENTER; OSTEOBLAST; PHENOTYPE; PHYSIOLOGY; REVIEW; SHORT DURATION; STEM CELL; STS SHUTTLE PROJECT; STS-56 SHUTTLE PROJECT; STS-76 SHUTTLE PROJECT; UNMANNED; WEIGHTLESSNESS;

NASA DISCIPLINE CELL BIOLOGY; NASA PROGRAM FUNDAMENTAL SPACE BIOLOGY; NON-NASA CENTER;

FLIGHT EXPERIMENT; FOTON PROJECT; MANNED; SHORT DURATION; STS SHUTTLE PROJECT; STS-56 SHUTTLE PROJECT; STS-76 SHUTTLE PROJECT; UNMANNED;

ANIMALS; CELL DIFFERENTIATION; CELL DIVISION; CYTOSKELETON; GENE EXPRESSION; HUMANS; MOLECULAR BIOLOGY; OSTEOBLASTS; PHENOTYPE; STEM CELLS; WEIGHTLESSNESS;

EID: 0141498008     PISSN: 15692574     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s1569-2574(02)08017-6     Document Type: Article

References (100)

1. P. Mack Bone density changes in a Macaca Nemestrina monkey during the Biosatellite II project Aerospace Med. 42 1971 828 833
2. A. Nicogossian C. Huntoon S. Pool Space Physiology and Medicine 1989 Lea and Febiger Philadephia
3. P.M.F. Vogt Roentgenographic bone density changes during representative Apollo space flight Am. J. Roentenol. 113 1971 621 623
4. G. Vose Review of roentgenographic bone demineralization studies of Gemini space flights Am. J. Roentgenol. 121 1975
5. P.R.R. Johnson Prolonged weightlessness and calcium loss in man Acta Astronautica 6 1979 1113 1122
6. C. Cann Determination of spine mineral density using computerized tomograms: a report: NASA CIF US/USSR Joint Working Group Meeting on Space Biology and Medicine 1981 9 22
7. E.R. Morey D.J. Baylink Inhibition of bone formation during space flight Science 201 1978 1138 1141
8. T.J. Wronski E.R. Morey Effect of spaceflight on periosteal bone formation in rats Am. J. Physiol. 244 1983 R305 R309
9. P. Collet D. Uebelhart L. Vico L. Moro D. Hartmann M. Roth C. Alexandre Effects of 1- and 6-month spaceflight on bone mass and biochemistry in two humans Bone 20 1997 547 551
10. V.S. Oganov L.I. Voronin A.S. Rakhmanov A.V. Bakulin V.S. Schneider A.D. Leblank Bone loss in microgravity Aviakosm. Ekolog. Med. 6 1972 20 24
11. A. Caillot-Augusseau M.H. Lafage-Proust C. Soler J. Pernod F. Dubois C. Alexandre Bone formation and resorption biological markers in cosmonauts during and after a 180-day space flight (Euromir 95) Clin. Chem. 44 1998 578 585
12. L. Vico P. Collet A. Guignandon M.H. Lafage-Proust T. Thomas M. Rehaillia C. Alexandre Effects of long-term microgravity exposure on cancellous and cortical weight-bearing bones of cosmonauts Lancet 355 2000 1607 1611
13. T.P. Stein M.J. Leskiw M.D. Schluter R.W. Hoyt H.W. Lane R.E. Gretebeck A.D. LeBlanc Energy expenditure and balance during spaceflight on the space shuttle Am. J. Physiol. 276 1999 R1739 R1748
14. T.P. Stein M.J. Leskiw M.D. Schluter M.R. Donaldson I. Larina Protein kinetics during and after long-duration spaceflight on MIR Am. J. Physiol. 276 1999 E1014 E1021
15. T.P. Stein M.D. Schluter M.J. Leskiw G. Boden Attenuation of the protein wasting associated with bed rest by branched-chain amino acids Nutrition 15 1999 656 660
16. T.P. Stein M.D. Schluter Plasma amino acids during human spaceflight Aviat. Space Environ. Med. 70 1999 250 255
17. T.P. Stein M.D. Schluter L.L. Moldawer Endocrine relationships during human spaceflight Am. J. Physiol. 276 1999 E155 E162
18. M.R. Forwood Inducible cyclo-oxygenase (COX-2) mediates the induction of bone formation by mechanical loading in vivo J. Bone Miner. Res. 11 1996 1688 1693
19. L.Y. Tang D.M. Cullen J.A. Yee W.S. Jee D.B. Kimmel Prostaglandin E2 increases the skeletal response to mechanical loading J. Bone Miner. Res. 12 1997 276 282
20. M. Hughes-Fulford R. Appel M. Kumegawa J. Schmidt Effect of dexa-methasone on proliferating osteoblasts: inhibition of prostaglandin E2 synthesis, DNA synthesis, and alterations in actin cytoskeleton Exp. Cell Res. 203 1992 150 156
21. C.T. Brighton J.R. Fisher Jr. S.E. Levine J.R. Corsetti T. Reilly A.S. Landsman J.L. Williams L.E. Thibault The biochemical pathway mediating the proliferative response of bone cells to a mechanical stimulus J. Bone Joint Surg. Am. 78 1996 1337 1347
22. D.D. Konieczynski M.J. Truty A.A. Biewener Evaluation of a bone's in vivo 24-hour loading history for physical exercise compared with background loading J. Orthop. Res. 16 1998 29 37
23. G.S. Stein J.B. Lian J.L. Stein A.J. Van Wijnen M. Montecino Transcriptional control of osteoblast growth and differentiation Physiol. Rev. 76 1996 593 629
24. A. Cogoli A. Tschopp P. Fuchs-Bislin Cell sensitivity to gravity Science 225 1984 228 230
25. A. Cogoli The effect of hypogravity and hypergravity on cells of the immune system J. Leukoc. Biol. 54 1993 259 268
26. A. Cogoli M. Cogoli-Greuter Activation and proliferation of lymphocytes and other mammalian cells in microgravity Adv. Space Biol. Med. 6 1997 33 79
27. M.L. Lewis J.L. Reynolds L.A. Cubano J.P. Hatton B.D. Lawless E.H. Piepmeier Spaceflight alters microtubules and increases apoptosis in human lymphocytes (Jurkat) FASEB J. 12 1998 1007 1018
28. M. Hughes-Fulford M.L. Lewis Effects of microgravity on osteoblast growth activation Exp. Cell Res. 224 1996 103 109
29. Y. Kumei H. Shimokawa H. Katano E. Hara H. Akiyama M. Hirano C. Mukai S. Nagaoka P.A. Whitson C.F. Sams Microgravity induces prostaglandin E2 and interleukin-6 production in normal rat osteoblasts: role in bone demineralization .J. Biotechnol. 47 1996 313 324
30. A. Guignandon Y. Usson N. Laroche M.H. Lafage-Proust O. Sabido C. Alexandre L. Vico Effects of intermittent or continuous gravitational stresses on cell-matrix adhesion: quantitative analysis of focal contacts in osteoblastic ROS 17/2.8 cells Exp. Cell Res. 236 1997 66 75
31. G. Carmeliet G. Nys I. Stockmans R. Bouillon Gene expression related to the differentiation of osteoblastic cells is altered by microgravity Bone 22 1998 139S 143S
32. W.J. Landis K.J. Hodgens D. Block C.D. Toma L.C. Gerstenfeld Space-flight effects on cultured embryonic chick bone cells J. Bone Miner. Res. 15 2000 1099 1112
33. M. Hughes-Fulford R. Tjandrawinata J. Fitzgerald K. Gasuad V. Gilbertson Effects of microgravity on osteoblast growth Gravit. Space Biol. Bull. 11 1998 51 60
34. M. Hughes-Fulford V.G. Gillbertson Microgravity caused decreased gene induction of growth factors, oncogenes and cos-2 in osteoblasts Abstracts from NASA cell biology meeting. Meeting Proceedings 2001
35. M. Hughes-Fulford Prostaglandin regulation of gene expression and growth in normal and malignant tissues Adv. Exp. Med. Biol. 1997 269 278
36. P.J. Marie D. Jones L. Vico A. Zallone M. Hinsenkamp R. Cancedda Osteobiology, strain, and microgravity: part I. Studies at the cellular level Calcif. Tissue Int. 67 2000 2 9
37. T. Kunisada A. Kawai H. Inoue M. Namba Effects of simulated micro-gravity on human osteoblast-like cells in culture Acta Med. Okayama 51 1997 135 140
38. D. Sarkar T. Nagaya K. Koga Y. Nomura R. Gruener H. Seo Culture in vector-averaged gravity under clinostat rotation results in apoptosis of osteoblastic ROS 17/2.8 cells J. Bone Miner. Res. 15 2000 489 498
39. E. Zerath D. Godet X. Holy C. Andre S. Renault M. Hott P.J. Marie Effects of spaceflight and recovery on rat humeri and vertebrae: histological and cell culture studies J. Appl. Physiol. 81 1996 164 171
40. E. Zerath X. Holy S.G. Roberts C. Andre S. Renault M. Hott P.J. Marie Spaceflight inhibits bone formation independent of corticosteroid status in growing rats J Bone Miner Res 15 2000 1310 1320
41. P. Montgomery J. Cox R. Reynolds J. Paul L. Hayflick D. Stock W. Shulz S. Kimzey The response of single human cells to zero gravity R.S. Johnson L.F. Dietlein Biomedical Results from Skylab 1977 Superintendent of Documents US Government Printing Office Washington, DC
42. M. Hughes-Fulford Altered cell function in microgravity Exp. Gerontol. 26 1991 247 256
43. M. Lewis M. Hughes-Fulford Cellular Responses to Microgravity 1997 Harvard University Cambridge, MA
44. A. Cogoli B. Bechler M. Cogoli-Greuter S.B. Criswell H. Joller P. Joller E. Hunzinger O. Muller Mitogenic signal transduction in T lymphocytes in microgravity J. Leukoc. Biol. 53 1993 569 575
45. A. Cogoli F.K. Gmunder Gravity effects on single cells: techniques, findings, and theory Adv. Space Biol. Med. 1 1991 183 248
46. A. Cogoli M. Valluchi-Morf M. Mueller W. Briegleb Effect of hypogravity on human lymphocyte activation Aviat. Space Environ. Med. 51 1980 29 34
47. J. Vassy S. Portet M. Beil G. Millot F. Fauvel-Lafeve A. Karniguian G. Gasset T. Irinopoulou F. Calvo J.P. Rigaut D. Schoevaert The effect of weight-lessness on cytoskeleton architecture and proliferation of human breast cancer cell line MCF-7 FASEB J. 15 2001 1104 1106
48. S.W. de Laat R.P. de Groot J. den Hertog P.J. Rijken A.J. Verkleij W. Kruijer J. Boonstra Epidermal growth factor induced signal transduction in A431 cells is influenced by altered gravity conditions Physiologist 35 1992 S19 S22
49. R.P. de Groot P.J. Rijken J. den Hertog J. Boonstra A.J. Verkleij S.W. de Laat W. Kruijer Microgravity decreases c-fos induction and serum response element activity J. Cell Sci. 97 1990 33 38
50. R.P. de Groot P.J. Rijken J. den Hertog J. Boonstra A.J. Verkleij S.W. de Laat W. Kruijer Nuclear responses to protein kinase C signal transduction are sensitive to gravity changes Exp. Cell Res. 197 1991 87 90
51. R.P. de Groot P.J. Rijken J. Boonstra A.J. Verkleij S.W. de Laat W. Kruijer Epidermal growth factor induced expression of c-fos is influenced by altered gravity conditions Aviat. Space Environ. Med. 62 1991 37 40
52. P.J. Rijken R.P. de Groot N. van Beizen S.W. de Laat J. Boonstra A.J. Verkleij Inhibition of EGF-induced signal transduction by microgravity is independent of EGF receptor redistribution in the plasma membrane of human A431 cells Exp. Cell Res. 204 1993 373 377
53. P.J. Rijken J. Boonstra A.J. Verkleij S.W. de Laat Effects of gravity on the cellular response to epidermal growth factor Adv. Space Biol. Med. 4 1994 159 188
54. J. Boonstra Growth factor-induced signal transduction in adherent mammalian cells is sensitive to gravity FASEB J. 13 1999 S35 S42
55. E. Hulleman J.J. Bijvelt A.J. Verkleij C.T. Verrips J. Boonstra Nuclear translocation of mitogen-activated protein kinase p42MAPK during the ongoing cell cycle J. Cell Physiol. 180 1999 325 333
56. E. Hulleman J. Boonstra Regulation of G1 phase progression by growth factors and the extracellular matrix Cell Mol. Life Sci. 58 2001 80 93
57. G.S. ran Rossum A.S. Vlug H. van Den Bosch A.J. Verkleij J. Boonstra Cytosolic phospholipase A(2) activity during the ongoing cell cycle J. Cell Physiol. 188 2001 321 328
58. A. Sato T. Hamazaki T. Oomura H. Osada M. Kakeya M. Watanabe T. Nakamura Y. Nakamura N. Koshikawa I. Yoshizaki S. Aizawa S. Yoda A. Ogiso M. Takaoki Y. Kohno H. Tanaka Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells Adv. Space Res. 24 1999 807 813
59. T.G. Hammond E. Benes K.C. O'Reilly D.A. Wolf R.M. Linnehan A. Taher J.H. Kaysen P.L. Allen T.J. Goodwin Mechanical culture conditions effect gene expression: gravity-induced changes on the space shuttle [In Process Citation] Physiol. Genomics 3 2000 163 173
60. T.G. Hammond F.C. Lewis T.J. Goodwin R.M. Linnehan D.A. Wolf K.P. Hire W.C. Campbell E. Benes K.C. O'Reilly R.K. Globus J.H. Kaysen Gene expression in space [letter] Nat. Med. 5 1999 359
61. M.L. Lewis L.A. Cubano B. Zhao H.K. Dinh J.G. Pabalan E.H. Piepmeier P.D. Bowman cDNA microarray reveals altered cytoskeletal gene expression in space-flown leukemic T lymphocytes (Jurkat) FASEB J 15 2001 1783 1785
62. A. Hughes-Fulford, M. (2001) unpublished observation.
63. M.R. Forwood W.L. Kelly N.F. Worth Localisation of prostaglandin endoperoxide H synthase (PGHS)-I and PGHS-2 in bone following mechanical loading in vivo Anat. Rec. 252 1998 580 586
64. M.R. Forwood C.H. Turner Skeletal adaptations to mechanical usage: results from tibial loading studies in rats Bone 17 1995 197S 205S
65. Y. Kumei H. Shimokawa H. Katano H. Akiyama M. Hirano C. Mukai S. Nagaoka P.A. Whitson C.F. Sams Spaceflight modulates insulin-like growth factor binding proteins and glucocorticoid receptor in osteoblasts J. Appl. Physiol. 85 1998 139 147
66. G. Carmeliet G. Nys R. Bouillon Microgravity reduces the differentiation of human osteoblastic MG-63 cells J. Bone Miner. Res. 12 1997 786 794
67. M. Chiquet Regulation of extracellular matrix gene expression by mechanical stress Matrix Biol. 18 1999 417 426
68. R.L. Duncan C.H. Turner Mechanotransduction and the functional response of bone to mechanical strain Calcif. Tissue Int. 57 1995 344 358
69. R.L. Duncan Transduction of mechanical strain in bone ASGSB Bull. 8 1995 49 62
70. X. Zhu M. Ohtsubo R.M. Bohmer J.M. Roberts R.K. Assoian Adhesion-dependent cell cycle progression linked to the expression of cyclin D1, activation of cyclin E-cdk2, and phosphorylation of the retinoblastoma protein J. Cell Biol. 133 1996 391 403
71. R.M. Bohmer E. Scharf R.K. Assoian Cytoskeletal integrity is required throughout the mitogen stimulation phase of the cell cycle and mediates the anchorage-dependent expression of cyclin D1 Mol. Biol. Cell 7 1996 101 111
72. A. Guignandon L. Vico C. Alexandre M.H. Lafage-Proust Shape changes of osteoblastic cells under gravitational variations during parabolic flight—relationship with PGE2 synthesis Cell Struct. Funct. 20 1995 369 375
73. P.J. Rijken W.J. Hage P.M. van Bergen en Henegouwen A.J. Verkleij J. Boonstra Epidermal growth factor induces rapid reorganization of the actin micro-filament system in human A431 cells J. Cell Sci. 100 1991 491 499
74. G. Carmeliet R. Bouillon The effect of microgravity on morphology and gene expression of osteoblasts in vitro FASEB J. 13 1999 S129 S134
75. M. Hughes-Fulford V. Gilbertson Osteoblast fibronectin mRNA, protein synthesis, and matrix are unchanged after exposure to microgravity FASEB J. 13 1999 S121 S127
76. M. Hughes-Fulford Changes in gene expression and signal transduction in microgravity J. Gravitat. Physiol. 8 1 2001 1 4
77. M. Hughes-Fulford R. Tjandrawinata J. Fitzgerald K. Gasuad V. Gilbertson Effect of microgravity on osteoblast growth activation: Analysis of transcription, translation and morphology on STS-Biorack on Spacehab 1999 ESA, Noordwijk The Netherlands
78. T.P. Stein C-peptide and PGE2 activity during human spaceflight Adv. Exp. Med. Biol. 407 1997 443 449
79. M. Hughes-Fulford Y. Chen R.R. Tjandrawinata Fatty acid regulates gene expression and growth of human prostate cancer PC-3 cells Carcinogenesis 22 2001 701 707
80. G. Reshetnikova R. Barkan B. Popov N. Nikolsky L.S. Chang Disruption of the actin cytoskeleton leads to inhibition of mitogen-induced cyclin E expression, Cdk2 phosphorylation, and nuclear accumulation of the retinoblastoma protein-related p107 protein Exp. Cell Res. 259 2000 35 53
81. F. Kheradmand E. Werner P. Tremble M. Symons Z. Werb Role of Racl and oxygen radicals in collagenase-1 expression induced by cell shape change Science 280 1998 898 902
82. A. Guignandon C. Genty L. Vico M.H. Lafage-Proust S. Palle C. Alexandre Demonstration of feasibility of automated osteoblastic line culture in space flight Bone 20 1997 109 116
83. C. Papaseit N. Pochon J. Tabony Microtubule self-organization is gravity-dependent Proc Nat. Acad. Sci. USA 97 2000 8364 8368
84. E.R. Morey-Holton R.K. Globus Hindlimb unloading of growing rats: a model for predicting skeletal changes during space flight Bone 22 1998 83S 88S
85. J.J. Van Loon D.J. Bervoets E.H. Burger S.C. Dieudonne J.W. Hagen C.M. Semeins B.Z. Doulabi J.P. Veldhuijzen Decreased mineralization and increased calcium release in isolated fetal mouse long bones under near weightlessness J. Bone Miner. Res. 10 1995 550 557
86. S.A. Harris M. Zhang L.S. Kidder G.L. Evans T.C. Spelsberg R.T. Turner Effects of orbital spaceflight on human osteoblastic cell physiology and gene expression Bone 26 2000 325 331
87. H. Akiyama S. Kanai M. Hirano H. Shimokawa H. Katano C. Mukai S. Nagaoka S. Morita Y. Kumei Expression of PDGF-beta receptor, EGF receptor, and receptor adaptor protein Shc in rat osteoblasts during spaceflight Mol. Cell Biochem. 202 1999 63 71
88. C.D. Toma S. Ashkar M.L. Gray J.L. Schaffer L.C. Gerstenfeld Signal transduction of mechanical stimuli is dependent on microfilament integrity: identification of osteopontin as a mechanically induced gene in osteoblasts J. Bone Miner. Res. 12 1997 1626 1636
89. F.M. Pavalko N.X. Chen C.H. Turner D.B. Burr S. Atkinson Y.F. Hsieh J. Qiu R.L. Duncan Fluid shear-induced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskeleton-integrin interactions Am. J. Physiol. 275 1998 C1591 C1601
90. L.E. Dike C.S. Chen M. Mrksich J. Tien G.M. Whitesides D.E. Ingber Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates In Vitro Cell Dev. Biol. Anim. 35 1999 441 448
91. S. Huang C.S. Chen D.E. Ingber Control of cyclin D1, p27(Kip1), and cell cycle progression in human capillary endothelial cells by cell shape and cytoskeletal tension Mol. Biol. Cell 9 1998 3179 3193
92. D.E. Ingber D. Prusty Z. Sun H. Betensky N. Wang Cell shape, cytoskeletal mechanics, and cell cycle control in angiogenesis J. Biomech. 28 1995 1471 1484
93. S.J. Sawyer S.M. Norvell S.M. Ponik F.M. Pavalko Regulation of PGE(2) and PGI(2) release from human umbilical vein endothelial cells by actin cytoskeleton Am. J. Physiol. Cell Physiol. 281 2001 C1038 C1045
94. M. Hughes-Fulford R. Tjandrawinata J. Fitzgerald K. Gasuad V.G. Gilbertson Effect of microgravity on osteoblast growth activation: Analysis of transcription, translation and morphology M. Perry Biorack on Spacehab: Biological Experiments on Three Shuttle-to-Mir Missions 1999 European Space Agency Noordwijk, The Netherlands 25 32
95. D.J. Baylink R.D. Finkelman S. Mohan Growth factors to stimulate bone formation J. Bone Miner. Res. 8 Suppl. 2 1993 S565 S572
96. L.E. Lanyon Using functional loading to influence bone mass and architecture: objectives, mechanisms, and relationship with estrogen of the mechanically adaptive process in bone Bone 18 1996 37S 43S
97. C. Neidlinger-Wilke H.J. Wilke L. Claes Cyclic stretching of human osteoblasts affects proliferation and metabolism: a new experimental method and its application J. Orthop. Res. 12 1994 70 78
98. J. Fitzgerald T.J. Dietz M. Hughes-Fulford Prostaglandin E2-induced up-regulation of c-fos messenger ribonucleic acid is primarily mediated by 3′,5′-cyclic adenosine monophosphate in MC3T3-E1 osteoblasts Endocrinology 141 2000 291 298
99. J. Wang K. Walsh Resistance to Apoptosis conferred by Cdk inhibitors during myocyte differentiation Science 273 1996 359 361
100. Hatton, J.P., Pooran, M., Li, C.-F., Luzzio, C. and Hughes-Fulford, M. A short pulse of mechanical force induces gene expression and growth in MC3T3-E1 osteoblasts via an ERK 1 2 pathway. JBMR, in press