Imaging of nuclear factor κB activation induced by ionizing radiation in human embryonic kidney (HEK) cells

Radiation and Environmental Biophysics

Volumn 53, Issue 3, 2014, Pages 599-610

  Chishti, Arif Ali ^a   Baumstark Khan, Christa ^a   Hellweg, Christine E ^a   Reitz, Günther ^a  

^a GERMAN AEROSPACE CENTER DLR   (Germany)

Author keywords

Fluorescent proteins; Ionizing radiation; Nuclear factor B; Reporter system; X rays

Indexed keywords

BINDING SITES; CELL DEATH; CELLS; CHEMICAL ACTIVATION; CYTOLOGY; ENZYME INHIBITION; FLUORESCENCE; FLUORESCENCE MICROSCOPY; IONIZING RADIATION; MAMMALS; MOBILE SECURITY; PATHOLOGY; PROTEINS; RADIATION; RADIATION SHIELDING; TRANSCRIPTION; TRANSCRIPTION FACTORS; X RAYS;

DOSE-DEPENDENT MANNER; FLUORESCENT PROTEIN; HUMAN EMBRYONIC KIDNEY CELLS; HUMAN EMBRYONIC KIDNEYS; NUCLEAR FACTOR KAPPAB; NUCLEAR FACTORS; RED FLUORESCENT PROTEINS; REPORTER SYSTEMS;

CELL SIGNALING;

MAMMALIA;

IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; PHOTOPROTEIN;

CELL PROLIFERATION; DNA DAMAGE; GENE EXPRESSION REGULATION; HEK293 CELL LINE; HUMAN; METABOLISM; MOLECULAR IMAGING; RADIATION RESPONSE; X RAY;

CELL PROLIFERATION; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, RADIATION; GENE EXPRESSION REGULATION; HEK293 CELLS; HUMANS; LUMINESCENT PROTEINS; MOLECULAR IMAGING; NF-KAPPA B; X-RAYS;

EID: 84925227879     PISSN: 0301634X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00411-014-0541-x     Document Type: Article

References (73)

1. Ashburner BP, Shackelford RE, Baldwin AS, Paules RS (1999) Lack of involvement of ataxia telangiectasia mutated (ATM) in regulation of nuclear factor-kappa B (NF-kappa B) in human diploid fibroblasts. Cancer Res 59:5456-5460
2. Baichwal VR, Baeuerle PA (1997) Activate NF-kappa B or die? Curr Biol 7:94-96
3. Baldwin AS (2001) Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappa B. J Clin Invest 107:241-246
4. Banaszynski LA, Chen L, Maynard-Smith LA, Ooi AGL, Wandless TJ (2006) A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Cell 126:995-1004
5. Basu S, Rosenzweig KR, Youmell M, Price BD (1998) The DNAdependent protein kinase participates in the activation of NF kappa B following DNA damage. Biochem Biophys Res Commun 247:79-83
6. Baumstark-Khan C, Hellweg CE, Palm M, Horneck G (2001) Enhanced green fluorescent protein (EGFP) for space radiation research using mammalian cells in the International Space Station. Phys Med Biol 17(Suppl 1):210-214
7. Baumstark-Khan C, Hellweg CE, Arenz A, Meier MM (2005) Cellular monitoring of the nuclear factor kappa B pathway for assessment of space environmental radiation. Radiat Res 164:527-530
8. Bhat-Nakshatri P, Sweeney CJ, Nakshatri H (2002) Identification of signal transduction pathways involved in constitutive NF-kappa B activation in breast cancer cells. Oncogene 21:2066-2078
9. Bora RS, Gupta D, Malik R, Chachra S, Sharma P, Saini KS (2008) Development of a cell-based assay for screening of phosphodiesterase 10A (PDE10A) inhibitors using a stable recombinant HEK-293 cell line expressing high levels of PDE10A. Biotechnol Appl Biochem 49:129-134
10. Brzoska K, Szumiel I (2008) Signalling loops and linear pathways: NF-j B activation in response to genotoxic stress. Mutagenesis 24:1-8
11. Cervera L, Gutierrez S, Godia F, Segura MM (2011) Optimization of HEK 293 cell growth by addition of non-animal derived components using design of experiments. BMC Proc 5:126
12. Chandel NS, Trzyna WC, McClintock DS, Schumacker PT (2000) Role of oxidants in NF-kappa B activation and TNF-alpha gene transcription induced by hypoxia and endotoxin. J Immunol 165:1013-1021
13. Chen X, Shen B, Xia L, Khaletzkiy A, Chu D, Wong Jeffrey YC, Li J (2002) Activation of nuclear factor kappa B in radioresistance of TP53-inactive human keratinocytes. Cancer Res 62:1213-1221
14. Fitzgerald DC, Meade KG, McEvoy AN, Lillis L, Murphy EP, MacHugh DE, Baird AW (2007) Tumour necrosis factor-a (TNF-a) increases nuclear factor j B (NFj B) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells. Vet Immunol Immunopath 116:59-68
15. Fuseler JW, Merrill DM, Rogers JA, Grisham MB, Wolf RE (2006) Analysis and quantitation of NF-kappa B nuclear translocation in tumor necrosis factor alpha (TNF-alpha) activated vascular endothelial cells. Microsc Microanal 12:269-276
16. Ghosh S, Hayden MS (2008) New regulators of NF-kappa B in inflammation. Nat Rev Drug Discov 8:837-848
17. Ghosh S, Hayden MS (2012) Celebrating 25 years of NF-kappa B research. Immunol Rev 246:5-13
18. Ghosh S, May MJ, Kopp EB (1998) NF-kappa B and rel proteins: evolutionarily conserved mediators of immune responses. Annu Rev Immunol 16:225-260
19. Ghosh S, Jimi E, Dong J, Zhong HH (2004) Regulation of NF-j B transcriptional activity. Shock 21:44
20. Grandage VL, Gale RE, Linch DC, Khwaja A (2005) PI3-kinase/Akt is constitutively active in primary acute myeloid leukaemia cells and regulates survival and chemoresistance via NF-kappa B, MAPkinase and p53 pathways. Leukemia 19:586-594
21. Habraken Y, Piette J (2006) NF-kappa B activation by double-strand breaks. Biochem Pharmacol 72:1132-1141
22. Hadian K, Krappmann D (2011) Signals from the nucleus: activation of NF-kappa B by cytosolic ATM in the DNA damage response. Sci Signal 4:e2
23. Hargrave BY, Tiangco DA, Lattanzio FA, Beebe SJ (2003) Cocaine, not morphine, causes the generation of reactive oxygen species and activation of NF-kappa B in transiently cotransfected heart cells. Cardiovasc Toxicol 3:141-151
24. Hayden MS (2004) Signaling to NF-j B. Genes Dev 18:2195-2224
25. Hayden MS, Ghosh S (2008) Shared principles in NF-kappa B signaling. Cell 132:344-362
26. Hellweg CE, Baumstark-Khan C, Horneck G (2003) Generation of stably transfected Mammalian cell lines as fluorescent screening assay for NF-kappa B activation-dependent gene expression. J Biomol Screen 8:511-521
27. Hellweg CE, Arenz A, Meier MM, Baumstark-Khan C (2005) Cellular monitoring systems for the assessment of space environmental factors. Adv Space Res 36:1673-1679
28. Hellweg CE, Baumstark-Khan C, Schmitz C, Lau P, Meier MM, Testard I, Berger T, Reitz G (2011) Carbon-ion-induced activation of the NF-j B pathway. Radiat Res 175:424-431
29. Huang TT, Wuerzberger-Davis S, Seufzer BJ, Shumway SD, Kurama T, Boothman DA, Miyamoto S (2000) NF-kappa B activation by camptothecin. A linkage between nuclear DNA damage and cytoplasmic signaling events. J Biol Chem 275:9501-9509
30. Janssens S, Tinel A, Lippens S, Tschopp J (2005) PIDD mediates NF-kappa B activation in response to DNA damage. Cell 123:1079-1092
31. Jin S, Lu D, Ye S, Ye H, Zhu L, Feng Z, Liu S, Wang D, Hu Q (2005) A simplified probe preparation for ELISA-based NF-kappa B activity assay. J Biochem Biophys Methods 65:20-29
32. Jung M, Dritschilo A (2001) NF-kappa B signaling pathway as a target for human tumor radiosensitization. Semin Radiat Oncol 11:346-351
33. Kawakami K, Scheidereit C, Roeder RG (1988) Identification and purification of a human immunoglobulin-enhancer-binding protein (Nf-jb) that activates transcription from a human immunodeficiency virus type-1 promoter in vitro. Proc Natl Acad Sci USA 85:4700-4704
34. Kiefer J (1971) Target theory and survival curves. J Theor Biol 30:307-331
35. Kim MJ, Pal S, Tak YK, Lee K, Yang TK, Lee S, Song JM (2007) Determination of the dose-depth distribution of proton beam using resazurin assay in vitro and diode laser-induced fluorescence detection. Anal Chim Acta 593:214-223
36. Lai C, Jiang X, Li X (2006) Development of luciferase reporter-based cell assays. Assay Drug Dev Technol 4:307-315
37. Lam LT, Davis R, Ngo VN, Lenz G, Wright G, Xu W, Zhao H, Yu X, Dang L, Staudt LM (2008) Compensatory IKK alpha activation of classical NF-kappa B signaling during IKK beta inhibition identified by an RNA interference sensitization screen. Proc Natl Acad Sci USA 105:20798-20803
38. Lau PN, Chow-Kevin BS, Chan C, Cheng-Christopher HK, Wise H (2009) The constitutive activity of the ghrelin receptor attenuates apoptosis via a protein kinase C-dependent pathway. Mol Cell Endocrinol 299:232-239
39. Leonore MLT (1999) Extracellular-regulated kinase 1/2, Jun N-terminal kinase, and c-jun are involved in NF-j B-dependent IL-6 expression in human monocytes. J Immunol 162:4893-4902
40. Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR (1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970-34975
41. Li N, Banin S, Ouyang H, Li GC, Courtois G, Shiloh Y, Karin M, Rotman G (2001) ATM is required for Ikappa B kinase (IKKk) activation in response to DNA double strand breaks. J Biol Chem 276:8898-8903
42. Lin Y, Ma W, Benchimol S (2000) Pidd, a new death-domaincontaining protein, is induced by p53 and promotes apoptosis. Nat Genet 26:122-127
43. Liu Z, Hazan-Halevy I, Harris DM, Li P, Ferrajoli A, Faderl S, Keating MJ, Estrov Z (2011) STAT-3 activates NF-j B in chronic lymphocytic leukemia cells. Mol Cancer Res 9:507-515
44. Mao W, Ye J, Guan Z, Zhao J, Zhang C, Zhang N, Jiang P, Tian T (2007) Cadmium induces apoptosis in human embryonic kidney (HEK) 293 cells by caspase-dependent and-independent pathways acting on mitochondria. Toxicol In Vitro 21:343-354
45. Matsuda M, Tsukiyama T, Bohgaki M, Nonomura K, Hatakeyama S (2007) Establishment of a newly improved detection system for NF-j B activity. Immunol Lett 109:175-181
46. Miyamoto S (2010) Nuclear initiated NF-j B signaling: NEMO and ATM take center stage. Cell Res 21:116-130
47. Mukogawa T, Koyama F, Tachibana M, Takayanagi A, Shimizu N, Fujii H, Ueno M, Matsumoto H, Takeuchi T, Nakajima Y (2003) Adenovirus-mediated gene transduction of truncated I kappa B alpha enhances radiosensitivity in human colon cancer cells. Cancer Sci 94:745-750
48. Muroi M, Tanamoto K (2008) TRAF6 distinctively mediates My D88-and IRAK-1-induced activation of NF-kappa B. J Leukoc Biol 83:702-707
49. Pessara U, Koch N (1990) Tumor necrosis factor alpha regulates expression of the major histocompatibility complex class IIassociated invariant chain by binding of an NF-kappa B-like factor to a promoter element. Mol Cell Biol 10:4146-4154
50. Piret B, Piette J (1996) Topoisomerase poisons activate the transcription factor NF-kappa B in ACH-2 and CEM cells. Nucleic Acids Res 24:4242-4248
51. Puck T, Marcus P (1956) Action of X-rays on mammalian cells. J Exp Med 103:653-666
52. Raju U, Gumin GJ, Tofilon PJ (2000) Radiation-induced transcription factor activation in the rat cerebral cortex. Int J Radiat Biol 76:1045-1053
53. Russell JS, Tofilon PJ (2002) Radiation-induced activation of nuclear factor-kappa B involves selective degradation of plasma membrane-associated I(kappa)B(alpha). Mol Biol Cell 13:3431-3440
54. Sahijdak WM, Yang CR, Zuckerman JS, Meyers M, Boothman DA (1994) Alterations in transcription factor binding in radioresistant human melanoma cells after ionizing radiation. Radiat Res 138:S47-S51
55. Sellmyer MA, Chen Lc, Egeler EL, Rakhit R, Wandless TJ (2012) Intracellular context affects levels of a chemically dependent destabilizing domain. Plos One 7
56. Sen R, Smale ST (2010) Selectivity of the NF-j B response. Perspect Biol 2:a000257
57. Shaner NC, Campbell RE, Steinbach PA, Giepmans Ben NG, Palmer AE, Tsien RY (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22:1567-1572
58. Shaner NC, Steinbach PA, Tsien RY (2005) A guide to choosing fluorescent proteins. Nat Meth 2:905-909
59. Solan NJ, Miyoshi H, Carmona EM, Bren GD, Paya CV (2002) Rel B cellular regulation and transcriptional activity are regulated by p100. J Biol Chem 277:1405-1418
60. Strongin DE, Bevis B, Khuong N, Downing ME, Strack RL, Sundaram K, Glick BS, Keenan RJ (2007) Structural rearrangements near the chromophore influence the maturation speed and brightness of Ds Red variants. Protein Eng Des Sel 20:525-534
61. Szumiel I (2008) Intrinsic radiation sensitivity: cellular signaling is the key. Radiat Res 169:249-258
62. Takemoto Y (1999) Increased JNK, AP-1 and NF-j B DNA binding activities in isoproterenol-induced cardiac remodeling. J Molec Cell Cardiol 31:2017-2030
63. Tamatani M, Che YH, Matsuzaki H, Ogawa S, Okado H, Miyake S, Mizuno T, Tohyama M (1999) Tumor necrosis factor induces Bcl-2 and Bcl-x expression through NFkappa B activation in primary hippocampal neurons. J Biol Chem 274:8531-8538
64. Treede I, Braun A, Jeliaskova P, Giese T, llekrug J, Griffiths G, Stremmel W, Ehehalt R (2009) TNF-a-induced up-regulation of pro-inflammatory cytokines is reduced by phosphatidylcholine in intestinal epithelial cells. BMC Gastroenterol 9:53
65. Troppmair J, Hartkamp J, Rapp UR (1998) Activation of NF-kappa B by oncogenic Raf in HEK 293 cells occurs through autocrine recruitment of the stress kinase cascade. Oncogene 17:685-690
66. Verma IM, Stevenson JK, Schwarz EM, van Antwerp D, Miyamoto S (1995) Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev 9:2723-2735
67. Volcic M, Karl S, Baumann B, Salles D, Daniel P, Fulda S, Wiesmuller L (2012) NF-kappa B regulates DNA double-strand break repair in conjunction with BRCA1-Ct IP complexes. Nucleic Acids Res 40:181-195
68. Watt PM, Hickson ID (1994) Structure and function of type II DNA topoisomerases. Biochem J 303:681-695
69. Westbrook AM, Wei B, Hacke K, Xia M, Braun J, Schiestl RH (2012) The role of tumour necrosis factor-alpha and tumour necrosis factor receptor signalling in inflammation-associated systemic genotoxicity. Mutagenesis 27:77-86
70. Wheelhouse NM, Chan YS, Gillies SE, Caldwell H, Ross JA, Harrison DJ, Prost S (2003) TNF-alpha induced DNA damage in primary murine hepatocytes. Int J Mol Med 12:889-894
71. Yang CR, Wilson-Van PC, Planchon SM, Wuerzberger-Davis SM, Davis TW, Cuthill S, Miyamoto S, Boothman DA (2000) Coordinate modulation of Sp1, NF-kappa B, and p53 in confluent human malignant melanoma cells after ionizing radiation. FASEB J 14:379-390
72. Yu Q, Rose JH, Zhang H, Pommier Y (2001) Antisense inhibition of Chk2/h Cds1 expression attenuates DNA damage-induced S and G2 checkpoints and enhances apoptotic activity in HEK-293 cells. FEBS Lett 505:7-12
73. Zhou D, Brown SA, Yu T, Chen G, Barve S, Kang BC, Thompson JS (1999) A high dose of ionizing radiation induces tissue-specific activation of nuclear factor-kappa B in vivo. Radiat Res 151:703-709