Super-resolution imaging for cell biologists: Concepts, applications, current challenges and developments Prospects & Overviews E. F. Fornasiero and F. Opazo

BioEssays

Volumn 37, Issue 4, 2015, Pages 436-451

  Fornasiero, Eugenio F ^a,b   Opazo, Felipe ^a,b  

^a EUROPEAN NEUROSCIENCE INSTITUTE   (Germany)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Affinity probes; Cell imaging; PALM; SIM; STED; STORM; Sub diffraction imaging

Indexed keywords

ARTICLE; CELL MEMBRANE POTENTIAL; CELL STRUCTURE; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CYTOLOGY; DIFFRACTION; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; ILLUMINATION; IMAGE ANALYSIS; IMMUNOFLUORESCENCE; MICROSCOPY; MULTIPHOTON MICROSCOPY; PHOTOACTIVATION; PHOTOACTIVATION LOCALIZATION MICROSCOPY; PHOTODEGRADATION; PHOTOTOXICITY; PROTEIN INTERACTION; STIMULATED EMISSION DEPLETION MICROSCOPY; STOCHASTIC OPTICAL RECONSTRUCTION MICROSCOPY; STRUCTURED ILLUMINATION MICROSCOPY; SUPER RESOLUTION MICROSCOPY; SYNAPTOSOME; THREE DIMENSIONAL IMAGING; PROCEDURES;

MICROSCOPY;

EID: 84925046499     PISSN: 02659247     EISSN: 15211878     Source Type: Journal    
DOI: 10.1002/bies.201400170     Document Type: Article

References (130)

1. Saka S, Rizzoli SO. 2012. Super-resolution imaging prompts re-thinking of cell biology mechanisms: selected cases using stimulated emission depletion microscopy. BioEssays 34: 386-95.
2. Anonymous, 2012. The quest for quantitative microscopy. Nat Methods 9: 627.
3. Cremer C, Masters BR. 2013. Resolution enhancement techniques in microscopy. Eur Phys J H 38: 281-344.
4. Abbe E. 1873. Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung. Arch Mikroskop Anat 9: 413-20.
5. Chalfie M, Tu Y, Euskirchen G, Ward WW, et al. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-5.
6. Hell SW, Wichmann J. 1994. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt Lett 19: 780-2.
7. Cremer C, Cremer T. 1978. Considerations on a laser-scanning-microscope with high resolution and depth of field. Microsc Acta 81: 31-44.
8. Sheppard CJ, Wilson T. 1981. The theory of the direct-view confocal microscope. J Microsc 124: 107-17.
9. Hell S, Stelzer EHK. 1992. Properties of a 4Pi confocal fluorescence microscope. J Opt Soc Am A 9: 2159.
10. Gustafsson MGL, Agard DA, Sedat JW. 1995. Sevenfold improvement of axial resolution in 3D wide-field microscopy using two objective lenses. Proc SPIE 2412: 147-56.
11. Willig KI, Rizzoli SO, Westphal V, Jahn R, et al. 2006. STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis. Nature 440: 935-9.
12. Hofmann M, Eggeling C, Jakobs S, Hell SW. 2005. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. Proc Natl Acad Sci USA 102: 17565-9.
13. Gustafsson MG. 2000. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J Microsc 198: 82-7.
14. Heintzmann R, Cremer CG. 1999. Laterally modulated excitation microscopy: improvement of resolution by using a diffraction grating. Proc SPIE 3568: 185-96.
15. Heintzmann R, Jovin TM, Cremer C. 2002. Saturated patterned excitation microscopy-a concept for optical resolution improvement. J Opt Soc Am A Opt Image Sci Vis 19: 1599-609.
16. Betzig E, Patterson GH, Sougrat R, Lindwasser OW, et al. 2006. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313: 1642-5.
17. Rust MJ, Bates M, Zhuang X. 2006. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3: 793-5.
18. Hess ST, Girirajan TPK, Mason MD. 2006. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys J 91: 4258-72.
19. Heilemann M, van de Linde S, Schüttpelz M, Kasper R, et al. 2008. Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. Angew Chem Int Ed Engl 47: 6172-6.
20. Reymann J, Baddeley D, Gunkel M, Lemmer P, et al. 2008. High-precision structural analysis of subnuclear complexes in fixed and live cells via spatially modulated illumination (SMI) microscopy. Chromosome Res 16: 367-82.
21. Lemmer P, Gunkel M, Baddeley D, Kaufmann R, et al. 2008. SPDM: light microscopy with single-molecule resolution at the nanoscale. Appl Phys B 93: 1-12.
22. Van de Linde S, Heilemann M, Sauer M. 2012. Live-cell super-resolution imaging with synthetic fluorophores. Annu Rev Phys Chem 63: 519-40.
23. Rolls MM, Stein Pa, Taylor SS, Ha E, et al. 1999. A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein. J Cell Biol 146: 29-44.
24. Stadler C, Rexhepaj E, Singan VR, Murphy RF, et al. 2013. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 10: 315-23.
25. Löschberger A, van de Linde S, Dabauvalle M-C, Rieger B, et al. 2012. Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution. J Cell Sci 125: 570-5.
26. Xu K, Zhong G, Zhuang X. 2013. Actin, spectrin, and associated proteins form a periodic cytoskeletal structure in axons. Science 339: 452-6.
27. Pageon SV, Cordoba S, Owen DM, Rothery SM, et al. 2013. Superresolution microscopy reveals nanometer-scale reorganization of inhibitory natural killer cell receptors upon activation of NKG2D. Sci Signal 6: ra62.
28. Opazo F, Punge A, Bückers J, Hoopmann P, et al. 2010. Limited intermixing of synaptic vesicle components upon vesicle recycling. Traffic 11: 800-12.
29. Schulz O, Pieper C, Clever M, Pfaff J, et al. 2013. Resolution doubling in fluorescence microscopy with confocal spinning-disk image scanning microscopy. Proc Natl Acad Sci USA 110: 21000-5.
30. Osseforth C, Moffitt J. 2014. Simultaneous dual-color 3D STED microscopy. Opt Express 22: 7028-39.
31. Huang B, Wang W, Bates M, Zhuang X. 2008. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science 319: 810-3.
32. Xu K, Babcock HP, Zhuang X. 2012. Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton. Nat Methods 9: 185-8.
33. Jia S, Vaughan JC, Zhuang X. 2014. Isotropic three-dimensional super-resolution imaging with a self-bending point spread function. Nat Photonics 8: 302-6.
34. Punge A, Rizzoli SO, Jahn R, Wildanger JD, et al. 2008. 3D reconstruction of high-resolution STED microscope images. Microsc Res Tech 71: 644-50.
35. Micheva KD, Smith SJ. 2007. Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits. Neuron 55: 25-36.
36. Nanguneri S, Flottmann B, Horstmann H, Heilemann M, et al. 2012. Three-dimensional, tomographic super-resolution fluorescence imaging of serially sectioned thick samples. PLoS ONE 7: e38098.
37. Cella Zanacchi F, Lavagnino Z, Perrone Donnorso M, Del Bue A et al. 2011. Live-cell 3D super-resolution imaging in thick biological samples. Nat Methods 8: 1047-79.
38. Chen B-C, Legant WR, Wang K, Shao L, et al. 2014. Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution. Science 346: 1257998.
39. Kaufmann R, Schellenberger P, Seiradake E, Dobbie IM, et al. 2014. Super-resolution microscopy using standard fluorescent proteins in intact cells under cryo-conditions. Nano Lett 14: 4171-5.
40. Gunkel M, Erdel F, Rippe K, Lemmer P, et al. 2009. Dual color localization microscopy of cellular nanostructures. Biotechnol J 4: 927-38.
41. Wong J, Baddeley D, Bushong Ea, Yu Z, et al. 2013. Nanoscale distribution of ryanodine receptors and caveolin-3 in mouse ventricular myocytes: dilation of t-tubules near junctions. Biophys J 104: L22-4.
42. Yau KW, van Beuningen SFB, Cunha-Ferreira I, Cloin BMC, et al. 2014. Microtubule Minus-End Binding Protein CAMSAP2 Controls Axon Specification and Dendrite Development. Neuron 82: 1058-73.
43. Wang Y, Maharana S, Wang MD, Shivashankar GV. 2014. Super-resolution microscopy reveals decondensed chromatin structure at transcription sites. Sci Rep 4: 4477.
44. Revelo NH, Kamin D, Truckenbrodt S, Wong AB, et al. 2014. A new probe for super-resolution imaging of membranes elucidates trafficking pathways. J Cell Biol 205: 591-606.
45. Saka SK, Honigmann A, Eggeling C, Hell SW, et al. 2014. Multi-protein assemblies underlie the mesoscale organization of the plasma membrane. Nat Commun 5: 1-14.
46. Wilhelm BG, Mandad S, Truckenbrodt S, Krohnert K, et al. 2014. Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins. Science 344: 1023-8.
47. Jungmann R, Avendaño MS, Woehrstein JB, Dai M, et al. 2014. Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT. Nat Methods 11: 313-8.
48. Lubeck E, Cai L. 2012. Single-cell systems biology by super-resolution imaging and combinatorial labeling. Nat Methods 9: 743-8.
49. Bethge P, Chéreau R, Avignone E, Marsicano G, et al. 2013. Two-photon excitation STED microscopy in two colors in acute brain slices. Biophys J 104: 778-85.
50. Gudheti MV, Curthoys NM, Gould TJ, Kim D, et al. 2013. Actin mediates the nanoscale membrane organization of the clustered membrane protein influenza hemagglutinin. Biophys J 104: 2182-92.
51. Magidson V, Khodjakov A. 2013. Circumventing photodamage in live-cell microscopy. Methods Cell Biol 114: 545-60.
52. Nanguneri S, Flottmann B, Herrmannsdörfer F, Thomas K, et al. 2014. Single-molecule super-resolution imaging by tryptophan-quenching-induced photoswitching of phalloidin-fluorophore conjugates. Microsc Res Tech 516: 510-6.
53. Westphal V, Rizzoli SO, Lauterbach MA, Kamin D, et al. 2008. Video-rate far-field optical nanoscopy dissects synaptic vesicle movement. Science 320: 246-9.
54. Holden SJ, Uphoff S, Kapanidis AN. 2011. DAOSTORM: an algorithm for high- density super-resolution microscopy. Nat Methods 8: 279-80.
55. Zhu L, Zhang W, Elnatan D, Huang B. 2012. Faster STORM using compressed sensing. Nat Methods 9: 721-3.
56. Huang F, Hartwich TMP, Rivera-Molina FE, Lin Y, et al. 2013. Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms. Nat Methods 10: 653-8.
57. Chen EH, Gaathon O, Trusheim ME, Englund D. 2013. Wide-field multispectral super-resolution imaging using spin-dependent fluorescence in nanodiamonds. Nano Lett 13: 2073-7.
58. Sahl SJ, Leutenegger M, Hell SW, Eggeling C. 2014. High-resolution tracking of single-molecule diffusion in membranes by confocalized and spatially differentiated fluorescence photon stream recording. ChemPhysChem 15: 771-83.
59. Vicidomini G, Moneron G, Han KY, Westphal V, et al. 2011. Sharper low-power STED nanoscopy by time gating. Nat Methods 8: 571-3.
60. Chmyrov A, Keller J, Grotjohann T, Ratz M, et al. 2013. Nanoscopy with more than 100, 000 "doughnuts". Nat Methods 10: 737-40.
61. Brown ACN, Dobbie IM, Alakoskela J-M, Davis I, et al. 2012. Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins. Blood 120: 3729-40.
62. York AG, Chandris P, Nogare DD, Head J, et al. 2013. Instant super-resolution imaging in live cells and embryos via analog image processing. Nat Methods 10: 1122-6.
63. Erdmann RS, Takakura H, Thompson AD, Rivera-Molina F, et al. 2014. Super-Resolution Imaging of the Golgi in Live Cells with a Bioorthogonal Ceramide Probe. Angew Chem Int Ed Engl 53: 10242-6.
64. Lukinavičius G, Reymond L, D'Este E, Masharina A, et al. 2014. Fluorogenic probes for live-cell imaging of the cytoskeleton. Nat Methods 11: 731-3.
65. Grimm JB, Sung AJ, Legant WR, Hulamm P, et al. 2013. Carbofluoresceins and carborhodamines as scaffolds for high-contrast fluorogenic probes. ACS Chem Biol 8: 1303-10.
66. Shannon CE. 1949. Communication in the presence of noise. Proc IRE 37: 10-21.
67. Fitzgerald JE, Lu J, Schnitzer MJ. 2012. Estimation Theoretic Measure of Resolution for Stochastic Localization Microscopy. Phys Rev Lett 109: 048102.
68. Ries J, Kaplan C, Platonova E, Eghlidi H, et al. 2012. A simple, versatile method for GFP-based super-resolution microscopy via nanobodies. Nat Methods 9: 582-4.
69. Opazo F, Levy M, Byrom M, Schäfer C, et al. 2012. Aptamers as potential tools for super-resolution microscopy. Nat Methods 9: 938-9.
70. Nieuwenhuizen RPJ, Lidke Ka, Bates M, Puig DL, et al. 2013. Measuring image resolution in optical nanoscopy. Nat Methods 10: 557-62.
71. Porter KR, Claude A, Fullam EF. 1945. A study of tissue culture cells by electron microscopy: methods and preliminary observations. J Exp Med 81: 233-46.
72. White JG, Amos WB, Fordham M. 1987. An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy. J Cell Biol 105: 41-8.
73. Hirvonen LM, Wicker K, Mandula O, Heintzmann R. 2009. Structured illumination microscopy of a living cell. Eur Biophys J 38: 807-12.
74. Markaki Y, Smeets D, Fiedler S, Schmid VJ, et al. 2012. The potential of 3D-FISH and super-resolution structured illumination microscopy for studies of 3D nuclear architecture. BioEssays 34: 412-26.
75. Müller T, Schumann C, Kraegeloh A. 2012. STED microscopy and its applications: new insights into cellular processes on the nanoscale. ChemPhysChem 13: 1986-2000.
76. Willig KI, Harke B, Medda R, Hell SW. 2007. STED microscopy with continuous wave beams. Nat Methods 4: 915-8.
77. Huang B, Jones SA, Brandenburg B, Zhuang X. 2008. Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution. Nat Methods 5: 1047-52.
78. Jones SA, Shim S-H, He J, Zhuang X. 2011. Fast, three-dimensional super-resolution imaging of live cells. Nat Methods 8: 499-508.
79. Nienhaus K, Nienhaus GU. 2014. Fluorescent proteins for live-cell imaging with super-resolution. Chem Soc Rev 43: 1088-106.
80. Hein B, Willig KI, Wurm Ca, Westphal V, et al. 2010. Stimulated emission depletion nanoscopy of living cells using SNAP-tag fusion proteins. Biophys J 98: 158-63.
81. Tanaka T, Yamamoto T, Tsukiji S, Nagamune T. 2008. Site-specific protein modification on living cells catalyzed by Sortase. ChemBioChem 9: 802-7.
82. Angelo M, Bendall SC, Finck R, Hale MB, et al. 2014. Multiplexed ion beam imaging of human breast tumors. Nat Med 20: 436-42.
83. Van den Bogaart G, Meyenberg K, Risselada HJ, Amin H, et al. 2011. Membrane protein sequestering by ionic protein-lipid interactions. Nature 479: 552-5.
84. Janas TT. 2011. The selection of aptamers specific for membrane molecular targets. Cell Mol Biol Lett 16: 25-39.
85. Mueller V, Ringemann C, Honigmann A, Schwarzmann G, et al. 2011. STED nanoscopy reveals molecular details of cholesterol- and cytoskeleton-modulated lipid interactions in living cells. Biophys J 101: 1651-60.
86. Hofmann K, Thiele C, Schött H-F, Gaebler A, et al. 2014. A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization. J Lipid Res 55: 583-91.
87. Chakraborty SK, Fitzpatrick JaJ, Phillippi Ja, Andreko S, et al. 2007. Cholera toxin B conjugated quantum dots for live cell labeling. Nano Lett 7: 2618-26.
88. Flors C. 2013. Super-resolution fluorescence imaging of directly labelled DNA: from microscopy standards to living cells. J Microsc 251: 1-4.
89. Zhang WI, Röhse H, Rizzoli SO, Opazo F. 2014. Fluorescent in situ hybridization of synaptic proteins imaged with super-resolution STED microscopy. Microsc Res Tech 527: 517-27.
90. Tyagi S. 2009. Imaging intracellular RNA distribution and dynamics in living cells. Nat Methods 6: 331-8.
91. Paige JS, Wu KY, Jaffrey SR. 2011. RNA mimics of green fluorescent protein. Science 333: 642-6.
92. Kasper R, Harke B, Forthmann C, Tinnefeld P, et al. 2010. Single-molecule STED microscopy with photostable organic fluorophores. Small 6: 1379-84.
93. Zessin PJM, Finan K, Heilemann M. 2012. Super-resolution fluorescence imaging of chromosomal DNA. J Struct Biol 177: 344-8.
94. Specht CG, Izeddin I, Rodriguez PC, El Beheiry M, et al. 2013. Quantitative nanoscopy of inhibitory synapses: counting gephyrin molecules and receptor binding sites. Neuron 79: 308-21.
95. Durisic N, Godin AG, Wever CM, Heyes CD, et al. 2012. Stoichiometry of the human glycine receptor revealed by direct subunit counting. J Neurosci 32: 12915-20.
96. Durisic N, Cuervo LL, Lakadamyali M. 2014. Quantitative super-resolution microscopy: pitfalls and strategies for image analysis. Curr Opin Chem Biol 20C: 22-8.
97. Annibale P, Vanni S, Scarselli M, Rothlisberger U, et al. 2011. Quantitative photo activated localization microscopy: unraveling the effects of photoblinking. PLoS One 6: e22678.
98. Bates M, Huang B, Dempsey GT, Zhuang X. 2007. Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science 317: 1749-53.
99. Puchner EM, Walter JM, Kasper R, Huang B, et al. 2013. Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory. Proc Natl Acad Sci USA 110: 16015-20.
100. Schmied JJ, Raab M, Forthmann C, Pibiri E, et al. 2014. DNA origami-based standards for quantitative fluorescence microscopy. Nat Protoc 9: 1367-91.
101. Deschout H, Cella Zanacchi F, Mlodzianoski M, Diaspro A, et al. 2014. Precisely and accurately localizing single emitters in fluorescence microscopy. Nat Methods 11: 253-66.
102. Porter RR. 1959. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J 73: 119-26.
103. Winter G, Griffiths AD, Hawkins RE, Hoogenboom HR. 1994. Making antibodies by phage display technology. Annu Rev Immunol 12: 433-55.
104. Hamers-Casterman C, Atarhouch T, Muyldermans S, Robinson G, et al. 1993. Naturally occurring antibodies devoid of light chains. Nature 363: 446-8.
105. Rothbauer U, Zolghadr K, Tillib S, Nowak D, et al. 2006. Targeting and tracing antigens in live cells with fluorescent nanobodies. Nat Methods 3: 887-9.
106. Ellington AD, Szostak JW. 1990. In vitro selection of RNA molecules that bind specific ligands. Nature 346: 818-22.
107. Nygren P-A. 2008. Alternative binding proteins: affibody binding proteins developed from a small three-helix bundle scaffold. FEBS J 275: 2668-76.
108. Binz HK, Stumpp MT, Forrer P, Amstutz P, et al. 2003. Designing Repeat Proteins: well-expressed, soluble and stable proteins from Combinatorial Libraries of Consensus Ankyrin Repeat Proteins. J Mol Biol 332: 489-503.
109. Koide A, Bailey CW, Huang X, Koide S. 1998. The fibronectin type III domain as a scaffold for novel binding proteins. J Mol Biol 284: 1141-51.
110. Milstein C. 1999. The hybridoma revolution: an offshoot of basic research. BioEssays 21: 966-73.
111. Clackson T, Hoogenboom HR, Griffiths AD, Winter G. 1991. Making antibody fragments using phage display libraries. Nature 352: 624-8.
112. Nord K, Nilsson J, Nilsson B. 1995. A combinatorial library of an α-helical bacterial receptor domain. Protein Eng 8: 601-8.
113. Jost C, Plückthun A. 2014. Engineered proteins with desired specificity: DARPins, other alternative scaffolds and bispecific IgGs. Curr Opin Struct Biol 27C: 102-12.
114. Hopwood D. 1969. Fixatives and fixation: a review. Histochem J 1: 323-60.
115. Hopwood D. 1972. Theoretical and practical aspects of glutaraldehyde fixation. Histochem J 4: 267-303.
116. Sabatini DD, Bensch K, Barrnett RJ. 1963. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol 17: 19-58.
117. Tanaka K, a K, Suzuki KGN, Shirai YM, Shibutani ST, et al. 2010. Membrane molecules mobile even after chemical fixation. Nat Methods 7: 865-6.
118. Reese TS, Karnovsky MJ. 1967. Fine structural localization of a blood-brain barrier to exogenous peroxidase. J Cell Biol 34: 207-17.
119. Matsubayashi Y, Iwai L, Kawasaki H. 2008. Fluorescent double-labeling with carbocyanine neuronal tracing and immunohistochemistry using a cholesterol-specific detergent digitonin. J Neurosci Methods 174: 71-81.
120. Heller I, Sitters G, Broekmans OD, Farge G, et al. 2013. STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA. Nat Methods 10: 910-6.
121. Chacko JV, Canale C, Harke B, Diaspro A. 2013. Sub-diffraction nano manipulation using STED AFM. PLoS ONE 8: e66608.
122. Watanabe S, Punge A, Hollopeter G, Willig KI, et al. 2011. Protein localization in electron micrographs using fluorescence nanoscopy. Nat Methods 8: 80-4.
123. Kopek BG, Shtengel G, Grimm JB, Clayton Da. et al. 2013. Correlative photoactivated localization and scanning electron microscopy. PLoS ONE 8: e77209.
124. Sochacki Ka, Shtengel G, van Engelenburg SB, Hess HF. et al. 2014. Correlative super-resolution fluorescence and metal-replica transmission electron microscopy. Nat Methods 11: 305-8.
125. Chang Y-W, Chen S, Tocheva EI, Treuner-Lange A, et al. 2014. Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods 11: 737-9.
126. Bálint Š, Verdeny Vilanova I, Sandoval Álvarez Á, Lakadamyali M. 2013. Correlative live-cell and superresolution microscopy reveals cargo transport dynamics at microtubule intersections. Proc Natl Acad Sci USA 110: 3375-80.
127. Saka SK, Vogts A, Kröhnert K, Hillion F, et al. 2014. Correlated optical and isotopic nanoscopy. Nat Commun 5: 3664.
128. Flottmann B, Gunkel M, Lisauskas T, Heilemann M, et al. 2013. Correlative light microscopy for high-content screening. Biotechniques 55: 243-52.
129. Smeets D, Markaki Y, Schmid VJ, Kraus F, et al. 2014. Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci. Epigenetics Chromatin 7: 8.
130. Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, et al. 2010. Nanoscale architecture of integrin-based cell adhesions. Nature 468: 580-4.