Video-rate far-field optical nanoscopy dissects synaptic vesicle movement

Science

Volumn 320, Issue 5873, 2008, Pages 246-249

  Westphal, Volker ^a   Rizzoli, Silvio O ^a,b   Lauterbach, Marcel A ^a   Kamin, Dirk ^b   Jahn, Reinhard ^a   Hell, Stefan W ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b EUROPEAN NEUROSCIENCE INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CROSS SECTION; DIFFRACTION; IMAGING METHOD; OPTICAL METHOD; PHYSIOLOGY;

ARTICLE; CELL FUNCTION; CELL MOTILITY; DIFFRACTION; FAR FIELD OPTICAL NANOSCOPY; FLUORESCENCE; HUMAN; HUMAN TISSUE; IMAGING SYSTEM; MICROSCOPY; NANOANALYSIS; NERVE CELL CULTURE; PRIORITY JOURNAL; STIMULATED EMISSION DEPLETION MICROSCOPY; SYNAPSE VESICLE; VIDEORECORDING;

ANIMALS; ANIMALS, NEWBORN; AXONS; CELLS, CULTURED; FLUORESCENT DYES; HIPPOCAMPUS; KINETICS; MICROSCOPY, FLUORESCENCE; MOVEMENT; NANOTECHNOLOGY; OPTICS; RATS; SYNAPTIC VESICLES; VIDEO RECORDING;

EID: 42049108013     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1154228     Document Type: Article

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29. S.O.R is the recipient of a long-term fellowship from the Human Frontier Science Program. This work was supported in part by funds from the Gottfried Wilhelm Leibniz Program of the Deutsche Forschungsgemeinschaft to R.J., by a grant from the German Ministry of Research and Education (BMBF, Nanolive) to S.W.H., and by a grant from the German Ministry of Research and Education (BMBF, Nanolive, "Vesikelbewegungen durch Nanoauflösung") to S.O.R. We thank I. Herfort for assistance with cell culturing and A. Di Nicola for his custom data-acquisition-card. We also thank L. Kastrup, J. Keller, and A. Schönle for helpful discussions and for help with the software ImSpector. Finally, we thank B. Rankin for critical reading of the manuscript.