Efficient selection of Gal-knockout pig cells for somatic cell nuclear transfer

Journal of Surgical Research

Volumn 184, Issue 2, 2013, Pages

  Reyes, Luz M ^a   Estrada, Jose L ^a   Ivary, Bess ^a   Sidner, Richard A ^a   Paris, Leela L ^a   Tector, A Joseph ^a  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Double selection; FACS; Gal KO; Magnetic beads; SCNT; Swine

Indexed keywords

ALPHA 1,3 GALACTOSYLTRANSFERASE; BIOTIN; FLUORESCENT DYE; LECTIN; STREPTAVIDIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL NUCLEUS TRANSPLANTATION; CELL SELECTION; CONJUGATE; CONTROLLED STUDY; FETUS; FETUS LIVER; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GENOTYPE; KNOCKOUT GENE; LIVER CELL; NONHUMAN; NULL CELL; PIGLET; PREGNANCY; PRIORITY JOURNAL; PRODUCTIVITY; RECIPIENT; SOMATIC CELL; TARGET CELL;

DOUBLE-SELECTION; FACS; GAL-KO; MAGNETIC BEADS; SCNT; SWINE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CELLS, CULTURED; FLOW CYTOMETRY; GALACTOSYLTRANSFERASES; LIVER; MAGNETICS; MODELS, ANIMAL; NUCLEAR TRANSFER TECHNIQUES; SWINE;

EID: 84883868847     PISSN: 00224804     EISSN: 10958673     Source Type: Journal    
DOI: 10.1016/j.jss.2012.11.054     Document Type: Article

References (12)

1. K. Kuwaki, Y.L. Tseng, and F.J. Dor Heart transplantation in baboons using 1,3-galactosyl transferase gene-knockout pigs as donors: initial experience Nat Med 11 2005 29
2. K. Yamada, K. Yazawa, and A. Shimizu Marked prolongation of porcine renal xenograft survival in baboons through the use of alpha1,3-galactosyltransferase gene-knockout donors and the cotransplantation of vascularized thymic tissue Nat Med 11 2005 32
3. D. Kolber-Simonds, L. Lai, and S.R. Watt Production of α-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations Proc Natl Acad Sci U S A 101 2004 7335
4. C.J. Phelps, C. Koike, and T.D. Vaught Production of α-1,3- galactosyltransferase-deficient pigs Science 299 2003 411
5. T. Fujimura, Y. Takahagi, and T. Shigehisa Production of alpha 1,3-galactosyltransferase gene-deficient pigs by somatic cell nuclear transfer: a novel selection method for Gal alpha 1,3-Gal antigen-deficient cells Mol Reprod Dev 75 2008 1372
6. J.A. Tischfield Loss of heterozygosity or: how I learned to stop worrying and love mitotic recombination Am J Hum Genet 61 1997 995
7. A. Richter Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer [dissertation] 2012 Ludwig-Maximilians-Universitât München Munich
8. S.K. Waghmare, J. Estrada, and L. Reyes Gene targeting and cloning in pigs using fetal liver derived cells J Surg Res 171 2011 e223
9. J. Estrada, J. Sommer, and B. Collins Swine generated by somatic cell nuclear transfer have increased incidence of intrauterine growth restriction (IUGR) Cloning Stem Cells 9 2007 229
10. C. Denning, P. Dickinson, S. Burl, D. Wylie, J. Fletcher, and A.J. Clark Gene targeting in sheep and pig primary fetal fibroblasts Cloning Stem Cells 3 2001 205
11. D.I. Jin, S.H. Lee, and J.H. Choi Targeting efficiency of α-1,3-galactosyl transferase gene in pig fetal fibroblast cells Exp Mol Med 35 2003 572
12. J. Ramsoondar, M. Mendicino, and C. Phelps Targeted disruption of the porcine immunoglobulin kappa light chain locus Transgenic Res 20 2011 643