Targeted disruption of the α1, 3-galactosyltransferase gene in cloned pigs

Nature Biotechnology

Volumn 20, Issue 3, 2002, Pages 251-255

  Dai, Yifan ^a   Vaught, Todd D ^a   Boone, Jeremy ^a   Chen, Shu Hung ^a   Phelps, Carol J ^a   Ball, Suyapa ^a   Monahan, Jeff A ^a   Jobst, Peter M ^a   McCreath, Kenneth J ^b   Lamborn, Ashley E ^a   Cowell Lucero, Jamie L ^a   Wells, Kevin D ^a   Colman, Alan ^b   Polejaeva, Irina A ^a   Ayares, David L ^a  

^a REVIVICOR INC   (United States)

^b PPL Therapeutics   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; FETAL MONITORING; GENES; TRANSPLANTATION (SURGICAL);

GENE ENCODING;

BIOTECHNOLOGY;

ALPHA 1,3 GALACTOSYLTRANSFERASE; GALACTOSYLGALACTOSE; GALACTOSYLTRANSFERASE; UNCLASSIFIED DRUG; EPITOPE; N ACETYLLACTOSAMINIDE ALPHA 1,3 GALACTOSYLTRANSFERASE; N-ACETYLLACTOSAMINIDE ALPHA-1,3-GALACTOSYLTRANSFERASE;

ALLELE; ANIMAL CELL; ARTICLE; CELL NUCLEUS TRANSPLANTATION; FEMALE; FIBROBLAST; GENE DISRUPTION; GRAFT REJECTION; MALE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SOUTHERN BLOTTING; SWINE; XENOGRAFT; XENOTRANSPLANTATION; ANIMAL; BIOLOGICAL MODEL; CELL LINE; CELL NUCLEUS; CLONING; GENETIC RECOMBINATION; GENETIC TRANSFECTION; GENETICS; METABOLISM; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

ANIMALS; BLOTTING, SOUTHERN; CELL LINE; CELL NUCLEUS; CLONING, ORGANISM; EPITOPES; FEMALE; FIBROBLASTS; GALACTOSYLTRANSFERASES; MALE; MODELS, GENETIC; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SWINE; TRANSFECTION;

EID: 0036009120     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0302-251     Document Type: Article

References (18)

1. Man, apes, and Old World monkeys differ from other mammals in the expression of α-galactosyl epitopes on nucleated cells
2. Xenotransplantation: The importance of the Galα 1, 3Gal epitope in hyperacute vascular rejection
3. The α-Gal epitope (Galα1-3Galβ1-4GlcNAc-R) in xenotransplantation
4. Galα(1, 3)Gal, the major xenoantigen(s) recognised in pigs by human natural antibodies
5. Human natural anti-α-galactosyl IgG II. The specific recognition of α(1-3)-linked galactose residues
6. The α-1, 3-galactosyltransferase knockout mouse. Implications for xenotransplantation
7. Oocyte Galα1, 3Gal epitopes implicated in sperm adhesion to the zona pellucida glycoprotein ZP3 are not required for fertilization in the mouse
8. Viable offspring derived from fetal and adult mammalian cells
9. Production of goats by somatic cell nuclear transfer
10. Cloned transgenic calves produced from nonquiescent fetal fibroblasts
11. Cloned pigs produced by nuclear transfer from adult somatic cells
12. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells
13. Deletion of the α(1, 3)galactosyl transferase (GGTA1) gene and the prion protein (PrP) gene in sheep
14. Production of α-1, 3-galactosyltransferase knockout pigs by nuclear transfer cloning
15. Problems encountered in detecting a targeted gene by the polymerase chain reaction
16. RNA template-specific PCR: An improved method that dramatically reduces false positives in RT-PCR
17. Nuclear transfer in cattle with non-transfected and transfected fetal or cloned transgenic fetal and postnatal fibroblasts
18. Differential expression of α-Gal epitopes (Galα-3Galβ1-4GlcNAc-R) on pig and mouse organs