Hot-spot analysis to dissect the functional protein-protein interface of a tRNA-modifying enzyme

Proteins: Structure, Function and Bioinformatics

Volumn 82, Issue 10, 2014, Pages 2713-2732

  Jakobi, Stephan ^a   Nguyen, Tran Xuan Phong ^a   Debaene, François ^b   Metz, Alexander ^a   Sanglier Cianférani, Sarah ^b   Reuter, Klaus ^a   Klebe, Gerhard ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

Crystal structure analysis; Interface binding hot spots; MM GBSA computational stability analysis; Native nano ESI mass spectrometry; Protein protein interaction inhibition; Site directed mutagenesis

Indexed keywords

GLYCOSYLTRANSFERASE; TRANSFER RNA; TRNA GUANINE TRANSGLYCOSYLASE; UNCLASSIFIED DRUG; ARCHAEAL PROTEIN; BACTERIAL PROTEIN; ESCHERICHIA COLI PROTEIN; ISOENZYME; MUTANT PROTEIN; QUEUINE TRNA-RIBOSYLTRANSFERASE; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; COMPUTER MODEL; CRYSTAL STRUCTURE; CRYSTALLIZATION; ELECTROSPRAY MASS SPECTROMETRY; ENZYME KINETICS; ENZYME LOCALIZATION; ENZYME STABILITY; GEL PERMEATION CHROMATOGRAPHY; HYDROGEN BOND; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; HYDROPHOBICITY; IN VITRO STUDY; MICHAELIS MENTEN KINETICS; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DATA BANK; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; SITE DIRECTED MUTAGENESIS; TIME OF FLIGHT MASS SPECTROMETRY; AMINO ACID SUBSTITUTION; BIOLOGY; CHEMICAL STRUCTURE; CHEMISTRY; DIMERIZATION; EXPERT SYSTEM; GENETICS; KINETICS; METABOLISM; PROTEIN CONFORMATION; PROTEIN DATABASE; PROTEIN DOMAIN;

AMINO ACID SUBSTITUTION; ARCHAEAL PROTEINS; BACTERIAL PROTEINS; COMPUTATIONAL BIOLOGY; DATABASES, PROTEIN; DIMERIZATION; ENZYME STABILITY; ESCHERICHIA COLI PROTEINS; EXPERT SYSTEMS; ISOENZYMES; KINETICS; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PENTOSYLTRANSFERASES; PROTEIN CONFORMATION; PROTEIN INTERACTION DOMAINS AND MOTIFS; RECOMBINANT PROTEINS; RNA, TRANSFER;

EID: 84922577430     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.24637     Document Type: Article

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