Purification, crystallization, and preliminary X-ray diffraction studies of tRNA-guanine transglycosylase from Zymomonas mobilis

Proteins: Structure, Function and Genetics

Volumn 24, Issue 4, 1996, Pages 516-519

  Romier, Christophe ^a   Ficner, Ralf ^a   Reuter, Klaus ^b   Suck, Dietrich ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

crystallization; queuosine; transglycosylation; tRNA; X ray diffraction

Indexed keywords

GLYCOSYLTRANSFERASE; GUANINE; TRANSFER RNA;

ANTICODON; ARTICLE; CELL FUNCTION; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME PURIFICATION; GLYCOSYLATION; METABOLIC REGULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; X RAY DIFFRACTION; ZYMOMONAS MOBILIS;

CHROMATOGRAPHY, ION EXCHANGE; CRYSTALLIZATION; MICROSCOPY, ELECTRON; PENTOSYLTRANSFERASES; RECOMBINANT PROTEINS; X-RAY DIFFRACTION; ZYMOMONAS;

EID: 0029897830     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0134(199604)24:4<516::AID-PROT11>3.0.CO;2-O     Document Type: Article

References (23)

1. Limbach, P.A., Crain, P.F., McCloskey, J.A. Summary: The modified nucleosides of RNA. Nucleic Acids Res. 22: 2183-2196, 1994.
2. Persson, B.C. Modification of tRNA as a regulatory device. Mol. Microbiol. 8:1011-1016, 1993.
3. Gehrke, C.W., Kuo, K.C.T. (eds.). Biosynthesis and function of queuine and queuosine in tRNAs. In: "Chromatography and Modification of Nucleosides. Biological Roles and Function of Modification." Part B. Amsterdam: Elsevier, 1993:69-108.
4. Durand, J.M., Okada, N., Tobe, T., Watarai, M., Fukuda, I., Suzuki, T., Nakata, N., Komatsu, K., Yoshikawa, M., Sasakawa, C. vacC, a virulence-associated chromosomal locus of Shigella flexneri, is homologous to tgt, a gene encoding tRNA-guanine transglycosylase (Tgt) of Escherichia coli K-12. J. Bacteriol. 176:4627-4634, 1994.
5. Katze, J.R., Gündüz, U., Smith, D.L., Cheng, C.S., McCloskey, J.A. Evidence that the nucleic acid base queuine is incorporated intact into tRNA by animal cells. Biochemistry 23:1171-1176, 1984.
6. Langgut, W. Regulation of signalling by receptor tyrosine kinases in HeLa cells involves the q-base. Biochem. Biophys. Res. Comm. 207:306-311, 1995.
7. Hatfield, D., Feng, Y.X., Lee, B.J., Rein, A., Levin, J.G., Oroszlan, S. Chromatographic analysis of the aminoacyl- tRNAs which are required for translation of codons at and around the ribosomal frameshift sites of HIV,HTLV-1, and BLV. Virology 173:736-742, 1989.
8. Slany, R.K., Kersten, H. Genes, enzymes and coenzymes of queuosine biosynthesis in procaryotes. Biochimie 76: 1178-1182, 1994.
9. Reuter, K., Slany, R., Ullrich, F., Kersten, H. Structure and organisation of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes. J. Bacteriol. 173:2256-2264, 1991.
10. Okada, N., Nishimura, S. Isolation and characterization of a guanme insertion enzyme, a specific tRNA transglycosylase, from Escherichia coli. J. Biol. Chem. 254:3061-3066, 1979.
11. Okada, N., Noguchi, S., Kasai, H., Shindo-Okada, N., Ohgi, T., Goto, T., Nishimura, S. Novel mechanism of posttranscriptional modification of tRNA. J. Biol. Chem. 254: 3067-3073, 1979.
12. Curnow, A.W., Kung, F.L., Koch, K.A., Garcia, G.A. tRNA-guanine transglycosylase from Escherichia coli: Gross tRNA structural requirements for recognition. Biochemistry 32:5239-5246, 1993.
13. Nakanishi, S., Ueda, T., Hori, H., Yamazaki, N., Okada, N., Watanabe, K. A UGU sequence in the anticodon loop is a minimum requirement for recognition by Escherichia coli tRNA-guanine transglycosylase. J. Biol. Chem. 269: 32221-32225, 1994.
14. Reuter, K., Ficner, R. Sequence analysis and overexpression of the Zymomonas mobilis gene encoding tRNA-guanine transglycosylase; purification and biochemical characterization of the enzyme. J. Bacteriol. 177:5284-5288, 1995.
15. Garcia, G.A., Koch, K.A., Chong, S. tRNA-guanine transglycosylase from Escherichia coli. Overexpression, purification and quaternary structure. J. Mol. Biol. 231:489-497, 1993.
16. Chong, S., Curnow, A.W., Huston, T.J., Garcia, G.A. tRNA-guanine transglycosylase from Escherichia coli is a zinc metalloprotein. Site-directed mutagenesis studies to identify the zinc ligands. Biochemistry 34:3694-3701, 1995.
17. Reuter, K., Chong, S., Ullrich, F., Kersten, H., Garcia, G.A. Serine 90 is required for enzymic activity by tRNA- guanine transglycosylase from Escherichia coli. Biochemistry 33:7041-7046, 1994.
18. Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248, 1976.
19. Kabsch, W. Evaluation of single-crystal X-ray diffraction data from a position-sensitive detector. J. Appl. Crystallogr. 21:916-924, 1988.
20. Zeelen, J.P., Hiltunen, J.K., Ceska, T.A., Wierenga, R.K. Crystallization experiments with 2-enoyl-CoA hydratase, using an automated "fast-screening" crystallization protocol. Acta Crystalogr. D50:443-447, 1994.
21. Ducruix, A., Giegé, R. (eds.). Crystallization in gels and related methods. In: "Crystallization of Nucleic Acids and Proteins: A Practical Approach." Oxford: Oxford University Press, 1993:127-143.
22. Ducruix, A., Giegé, R. (eds.). Seeding techniques. In: "Crystallization of Nucleic Acids and Proteins: A Practical Approach." Oxford: Oxford University Press, 1993:99-126.
23. Matthews, B.W. Solvent content of protein crystals. J. Mol. Biol. 33:491-497, 1968.