Efficient production of lycopene in Saccharomyces cerevisiae by expression of synthetic crt genes from a plasmid harboring the ADH2 promoter

Plasmid

Volumn 72, Issue 1, 2014, Pages 18-28

  Bahieldin, Ahmed ^a,b   Gadalla, Nour O ^a,c   Al Garni, Saleh M ^a   Almehdar, Hussein ^a   Noor, Samah ^a   Hassan, Sabah M ^a,b   Shokry, Ahmed M ^a,d   Sabir, Jamal S M ^a   Murata, Norio ^e  

^a KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

^b Ain Shams University   (Egypt)

^c NATIONAL RESEARCH CENTRE   (Egypt)

^d Agricultural Research Centre   (Egypt)

^e NATIONAL INSTITUTE FOR BASIC BIOLOGY   (Japan)

Author keywords

ADH2 promoter; Crt gene; Ergosterol; Lycopene; Saccharomyces cerevisiae; Vector dualsystem

Indexed keywords

ERGOSTEROL; GLUCOSE; LYCOPENE; CAROTENOID; FARNESYL TRANS TRANSFERASE; OXIDOREDUCTASE; PHYTOENE DEHYDROGENASE; PHYTOENE SYNTHASE;

ADH2 GENE; AMINO ACID SEQUENCE; ARTICLE; CODON USAGE; CONTROLLED STUDY; CRTB GENE; CRTE GENE; CRTI GENE; DEPLETION; DRY WEIGHT; ERWINIA; ERWINIA UREDOVORA; FUNGAL CELL; FUNGAL GENE; FUNGAL METABOLISM; FUNGAL STRAIN; FUNGUS GROWTH; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE OVEREXPRESSION; GENETIC TRANSCRIPTION; NONHUMAN; PLASMID; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSLATION; SACCHAROMYCES CEREVISIAE; STEROL SYNTHESIS; VITAMIN METABOLISM; WESTERN BLOTTING; BACTERIAL GENE; BIOSYNTHESIS; ENZYMOLOGY; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR CLONING; TRANSGENIC ORGANISM;

CAROTENOIDS; CLONING, MOLECULAR; ERGOSTEROL; ERWINIA; FARNESYLTRANSTRANSFERASE; GENE EXPRESSION; GENES, BACTERIAL; GERANYLGERANYL-DIPHOSPHATE GERANYLGERANYLTRANSFERASE; ORGANISMS, GENETICALLY MODIFIED; OXIDOREDUCTASES; PLASMIDS; PROMOTER REGIONS, GENETIC; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

EID: 84922278421     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2014.03.001     Document Type: Article

References (42)

1. Arroyo-López F.N., Orlić S., Querol A., Barrio E. Effects of temperature, pH and sugar concentration on the growth parameters of Saccharomyces cerevisiae, S. kudriavzevii and their interspecific hybrid. Int. J. Food Microbiol. 2009, 131:120-127. 10.1016/j.ijfoodmicro.2009.01.035.
2. Astorg P. Food carotenoids and cancer prevention: an overview of current research. Trends Food Sci. Technol. 1997, 8:406-413. 10.1016/S0924-2244(97)01092-3.
3. Boze H., Moulin G., Galzy P. Production of food and fodder yeasts. Crit. Rev. Biotechnol. 1992, 12:65-86. 10.3109/07388559209069188.
4. Charoenchai C., Fleet G., Henschke P.A. Effects of temperature, pH and sugar concentration on the growth rates and cell biomass of wine yeasts. Am. J. Enol. Viticulture 1998, 49:283-288.
5. Clinton S.K. Lycopene: chemistry, biology, and implications for human health and disease. Nutr. Rev. 1998, 56:35-51. 10.1111/j.1753-4887.1998.tb01691.
6. Farmer W.R., Liao J.C. Improving lycopene production in Escherichia coli by engineering metabolic control. Nat. Biotechnol. 2000, 18:533-537. 10.1038/75398.
7. Farmer W.R., Liao J.C. Precursor balancing for metabolic engineering of lycopene production in Escherichia coli. Biotechnol. Prog. 2001, 17:57-61. 10.1021/bp000137t.
8. Fleet G.H., Heard G.M. Yeasts-growth during fermentation. Wine Microbiology and Biotechnology 1993, 42-43. Harwood Academic Publishers, Chur, Switzerland. G.H. Fleet (Ed.).
9. Gancedo J. Yeast carbon catabolite repression. Microbiol. Mol. Biol. Rev. 1998, 62:334-361.
10. Ichii T., Takenaka S., Konno H., Ishida T., Sato H., et al. Development of a new commercial-scale airlift fermentor for rapid growth of yeast. J. Ferment. Bioeng. 1993, 75:375-379.
11. Jones K.L., Kim S.W., Keasling J.D. Low-copy plasmids can perform as well as or better than high-copy plasmids for metabolic engineering of bacteria. Metab. Eng. 2000, 2:328-338. 10.1006/mben.2000.0161.
12. Kang M.J., Lee Y.M., Yoon S.H., Kim J.H., Ock S.W., et al. Identification of genes affecting lycopene accumulation in Escherichia coli using a shot-gun method. Biotechnol. Bioeng. 2005, 91:636-642. 10.1002/bit.20539.
13. Kim S.W., Keasling J.D. Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol. Bioeng. 2001, 72:408-415. 10.1002/1097-0290(20000220)72:4<402.
14. Kitade Y., Watanabe S., Masaki T., Nishioka M., Nishino H. Inhibition of liver fibrosis in LEC rats by a carotenoid, lycopene, or herbal medicine, Sho-saiko-to. Hepatol. Res. 2002, 22:196-205. 10.1016/S1386-6346(01)00132-2.
15. Kushnirov V.V. Rapid and reliable protein extraction from yeast. Yeast 2000, 16:857-860. 10.1002/1097-0061(20000630)16:9<857.
16. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685. 10.1038/227680a0.
17. Lee K.M., DaSilva N.A. Evaluation of the Saccharomyces cerevisiae ADH2 promoter for protein synthesis. Yeast 2005, 22:431-440. 10.1002/yea.1221.
18. Lee P.C., Mijts B.N., Schmidt-Dannert C. Investigation of factors influencing production of the monocyclic carotenoid torulene in metabolically engineered Escherichia coli. Appl. Microbiol. Biotechnol. 2004, 65:538-546. doi: 07/s00253-004-1619-1.
19. Lotan T., Hirschberg J. Cloning and expression in Escherichia coli of the gene encoding beta-C-4-oxygenase, that converts beta-carotene to the ketocarotenoid canthaxanthin in Haematococcus pluvialis. FEBS Lett. 1995, 364:125-128. 10.1016/0014-5793(95)00368-J.
20. Misawa N., Shimada H. Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts. J. Biotechnol. 1997, 59:169-181. 10.1016/S0168-1656(97)00154-5.
21. Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., et al. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 1990, 172:6704-6712.
22. Miura Y., Kondo K., Saito T., Shimada H., Fraser P.D., et al. Production of the carotenoids lycopene, β-carotene, and astaxanthin in the food yeast Candida utilis. Appl. Environ. Microbiol. 1998, 64:1226-1229.
23. Miura Y., Kondo K., Shimada H., Saito T., Nakamura K., et al. Production of lycopene by the food yeast, Candida utilis that does not naturally synthesize carotenoid. Biotechnol. Bioeng. 1998, 58:306-308. 10.1002/(SICI)1097-0290(19980420)58:2/3<306.
24. Nadeem S.G., Shafiq A., Hakim S.T., Anjum Y., Kazm S.U. Effect of growth media, pH and temperature on yeast to hyphal transition in Candida albicans. Open J. Med. Microbiol. 2013, 3:185-192. 10.4236/ojmm.2013.33028.
25. Olson J.A. Carotenoids, vitamin A and cancer. J. Nutr. 1986, 116:1127-1130.
26. Price V., Taylor W., Clevenger W., Worthington M., Young E. Expression of heterologous proteins in Saccharomyces cerevisiae using the ADH2 promoter. Methods Enzymol. 1990, 185:308-318.
27. Rao A.V. Lycopene, tomatoes, and the prevention of coronary heart disease. Exp. Biol. Med. 2002, 227:908-913.
28. Rao A.V., Agarwal S. Role of antioxidant lycopene in cancer and heart disease. J. Am. College Nutr. 2000, 19:563-569. 10.1080/07315724.2000.10718953.
29. Richelle M., Bortlik K., Liardet S., Hager C., Lambelet P., et al. A food-based formulation provides lycopene with the same bioavailability to humans as that from tomato paste. J. Nutr. 2002, 132:404-408.
30. Ruther A., Misawa N., Boger P., Sandmann G. Production of zeaxanthin in Escherichia coli transformed with different carotenogenic plasmids. Appl. Microbiol. Biotechnol. 1997, 48:162-167. 10.1007/s002530051032.
31. Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning: a laboratory manual 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 2nd ed.
32. Sandmann G. Novel carotenoids genetically engineered in a heterologous host. Chem. Biol. 2003, 10:478-479. 10.1016/S1074-5521(03)00131-5.
33. Schmidt-Dannert C., Umeno D., Arnold F.H. Molecular breeding of carotenoid biosynthetic pathways. Nat. Biotechnol. 2000, 18:750-753. 10.1038/77319.
34. Schunemann H.J., McCann S., Grant B.J.B., Trevisan M., Muti P., et al. Lung function in relation to intake of carotenoid and other antioxidant vitamins in a population-based study. Am. J. Epidemiol. 2002, 155:463-471. 10.1093/aje/155.5.463.
35. Sedjo R.L., Roe D.J., Abrahamsen M., Harris R.B., Craft N., et al. Vitamin A, carotenoids and risk of persistent oncogenic human papillomavirus infection. Cancer Epidemiol. Biomarkers Prev. 2002, 11:876-884.
36. Sharma J.B., Kumar A., Kumar A., Malhotra M., Arora R., et al. Effect of lycopene on pre-eclampsia and intra-uterine growth retardation in primigravidas. Int. J. Gynecol. Obstet. 2003, 81:257-262. 10.1016/S0020-7292(03)00040-7.
37. Sharoni Y., Danielenko M., Walfisch S., Amir H., Nahum A., et al. Role of gene regulation in the anticancer activity of carotenoids. Pure Appl. Chem. 2002, 74:1469-1477. 10.1351/pac200274081469.
38. Sherman F., Fink G.R., Hicks J. Methods in Yeast Genetics 1982, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
39. Shi J., Le Maguer M. Lycopene in tomatoes: chemical and physical properties affected by food processing. Crit. Rev. Food Sci. Nutr. 2000, 20:293-334. 10.1080/10408690091189275.
40. Stahl W., Sies H. Carotenoids and protection against solar UV radiation. Skin Pharmacol. Appl. Skin Physiol. 2002, 15:291-296. 10.1159/000064532.
41. Wang G.-S., Grammel H., Abou-Aisha K. High-level production of the industrial product lycopene by the photosynthetic bacterium Rhodospirillum rubrum. Appl. Environ. Microbiol. 2012, 78:7205-7215. 10.1128/AEM.00545-12.
42. Yamano S., Ishii T., Nakagawa M., Ikenaga H., Misawa N. Metabolic engineering for production of beta-carotene and lycopene in Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. 1994, 58:1112-1114.