Evolution of the active site of human glutathione transferase A2-2 for enhanced activity with dietary isothiocyanates

Biochimica et Biophysica Acta - General Subjects

Volumn 1850, Issue 4, 2015, Pages 742-749

  Zhang, Wei ^a,c   Dourado, Daniel F A R ^d   Mannervik, Bengt ^a,b,c  

^a UPPSALA UNIVERSITY   (Sweden)

^b STOCKHOLM UNIVERSITY   (Sweden)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

^d UPPSALA UNIVERSITY   (Sweden)

Author keywords

Detoxication; Directed evolution; Enzyme engineering; Glutathione transferase; Isothiocyanate

Indexed keywords

ENZYME VARIANT; GLUTATHIONE TRANSFERASE A2; GLUTATHIONE TRANSFERASE A2 2; GLYCINE; HISTIDINE; ISOTHIOCYANIC ACID; MUTANT PROTEIN; UNCLASSIFIED DRUG; GLUTATHIONE S-TRANSFERASE ALPHA; GLUTATHIONE TRANSFERASE; ISOENZYME; ISOTHIOCYANIC ACID DERIVATIVE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DETOXIFICATION; DNA LIBRARY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE COMPLEX; MOLECULAR DOCKING; MOLECULAR EVOLUTION; MOLECULAR MODEL; MUTAGENESIS; PROTEIN EXPRESSION; CATALYSIS; CHEMISTRY; DIET; ENZYME SPECIFICITY; HUMAN; METABOLISM;

HEXAPODA; MAMMALIA;

CATALYSIS; CATALYTIC DOMAIN; DIET; GLUTATHIONE TRANSFERASE; HUMANS; ISOENZYMES; ISOTHIOCYANATES; SUBSTRATE SPECIFICITY;

EID: 84921327786     PISSN: 03044165     EISSN: 18728006     Source Type: Journal    
DOI: 10.1016/j.bbagen.2014.12.021     Document Type: Article

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