An efficient strategy for broad-range detection of low abundance bacteria without DNA decontamination of PCR reagents

PLoS ONE

Volumn 6, Issue 5, 2011, Pages

  Chang, Shy Shin ^a,b   Hsu, Hsung Ling ^c   Cheng, Ju Chien ^d   Tseng, Ching Ping ^c  

^a CHANG GUNG UNIVERSITY COLLEGE OF MEDICINE   (Taiwan)

^b CHANG GUNG MEMORIAL HOSPITAL   (Taiwan)

^c CHANG GUNG UNIVERSITY   (Taiwan)

^d CHINA MEDICAL UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; COMPLEMENTARY DNA; DNA DIRECTED DNA POLYMERASE BETA; EXONUCLEASE; EXONUCLEASE 1; GENOMIC DNA; RIBOSOME RNA; RNA 16S; UNCLASSIFIED DRUG; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; PRIMER DNA; TAQ POLYMERASE;

ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA CONTAMINATION; DNA DETERMINATION; DNA FINGERPRINTING; DNA SEQUENCE; DNA TEMPLATE; FALSE POSITIVE RESULT; GENE PROBE; HIGH RESOLUTION MELTING ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROCESS OPTIMIZATION; REAL TIME POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; BACTERIUM; DNA DENATURATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYMERASE CHAIN REACTION; SPECIES DIFFERENCE; WASTE MANAGEMENT;

BACTERIA (MICROORGANISMS); STAPHYLOCOCCUS AUREUS;

BACTERIA; DECONTAMINATION; DNA PRIMERS; DNA, BACTERIAL; INDICATORS AND REAGENTS; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; SPECIES SPECIFICITY; TAQ POLYMERASE;

EID: 79957556739     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0020303     Document Type: Article

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