Development of an ethidium monoazide-enhanced internally controlled universal 16S rDNA real-time polymerase chain reaction assay for detection of bacterial contamination in platelet concentrates

Transfusion

Volumn 52, Issue 7, 2012, Pages 1423-1432

  Patel, Poorvi ^a,b,c   Garson, Jeremy A ^a,b,c   Tettmar, Kate I ^a,b,c   Ancliff, Siobhan ^a,b,c   McDonald, Carl ^a,b,c   Pitt, Tyrone ^a,b,c   Coelho, Juliana ^a,b,c   Tedder, Richard S ^a,b,c  

^a NHS BLOOD AND TRANSPLANT   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

^c PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; DNA 16S; DNA POLYMERASE; ETHIDIUM; ETHIDIUM AZIDE; MITOCHONDRIAL DNA; RIBOSOME DNA; THROMBOCYTE CONCENTRATE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS CEREUS; BACTERIUM CONTAMINATION; BACTERIUM IDENTIFICATION; CLOSTRIDIUM PERFRINGENS; COLONY FORMATION; CONTROLLED STUDY; DNA CONTAMINATION; DNA ISOLATION; ENTEROBACTER CLOACAE; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; GENE AMPLIFICATION; HUMAN; HUMAN CELL; KLEBSIELLA PNEUMONIAE; LIMIT OF DETECTION; MALE; NONHUMAN; PHOTOACTIVATION; PROCESS OPTIMIZATION; PROPIONIBACTERIUM ACNES; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS PUTIDA; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; SERRATIA LIQUEFACIENS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS; STREPTOCOCCUS; STREPTOCOCCUS PNEUMONIAE; ULTRAFILTRATION;

AZIDES; BACTERIA; BLOOD PLATELETS; DNA, BACTERIAL; DNA, RIBOSOMAL; HUMANS; PLATELET TRANSFUSION; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S;

EID: 84863808334     PISSN: 00411132     EISSN: 15372995     Source Type: Journal    
DOI: 10.1111/j.1537-2995.2011.03484.x     Document Type: Article

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