Identification of mixed bacterial DNA contamination in broad-range PCR amplification of 16S rDNA V1 and V3 variable regions by pyrosequencing of cloned amplicons

FEMS Microbiology Letters

Volumn 219, Issue 1, 2003, Pages 87-91

  Grahn, Niclas ^a   Olofsson, Margaretha ^a   Ellnebo Svedlund, Katarina ^a   Monstein, Hans Jürg ^a,b   Jonasson, Jon ^a  

^a LINKÖPING UNIVERSITY HOSPITAL   (Sweden)

^b LINKÖPING UNIVERSITY   (Sweden)

Author keywords

16S rRNA; Bacterial DNA contamination; Pyrosequencing

Indexed keywords

BACTERIAL DNA; RNA 16S;

AMPLICON; ARTICLE; BACILLUS; BACTERIUM CONTAMINATION; BACTERIUM IDENTIFICATION; DNA SEQUENCE; GENE AMPLIFICATION; INDUSTRY; MICROFLORA; MOLECULAR BIOLOGY; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS; RALSTONIA; STENOTROPHOMONAS; TECHNIQUE; WATER CONTAMINATION; WATER SUPPLY; XANTHOMONAS;

BACILLUS; BACTERIA (MICROORGANISMS); PSEUDOMONAS; RALSTONIA; STENOTROPHOMONAS; XANTHOMONAS;

EID: 0037436159     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)01190-4     Document Type: Article

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