Multivariate modular engineering of the protein secretory pathway for production of heterologous glucose oxidase in Pichia pastoris

Enzyme and Microbial Technology

Volumn 68, Issue , 2015, Pages 33-42

  Gu, Lei ^a   Zhang, Juan ^a   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Glucose oxidase; Heterologous protein secretion; Modular engineering; Pichia pastoris

Indexed keywords

CELL MEMBRANES; CYTOLOGY; ELECTRIC RESISTANCE; GENES; GLUCOSE; GLUCOSE OXIDASE; GLUCOSE SENSORS; MACHINERY; PHYSIOLOGY; YEAST;

COMBINATION STRATEGIES; ENDOPLASMIC RETICULUM; HETEROLOGOUS PROTEIN SECRETION; HETEROLOGOUS PROTEINS; LARGE-SCALE PRODUCTION; PICHIA PASTORIS; PROTEIN SECRETORY PATHWAY; STRESS RESPONSE GENES;

PROTEINS;

GLUCOSE OXIDASE; SECRETORY PROTEIN; SYNTAXIN; TRANSCRIPTION FACTOR GCN4; UBIQUITIN CONJUGATING ENZYME 13; FUNGAL PROTEIN; HYBRID PROTEIN;

ARTICLE; CELL GROWTH; CONTROLLED STUDY; CYTOPLASM; ENDOPLASMIC RETICULUM; ENDOPLASMIC RETICULUM ASSOCIATED DEGRADATION; ENDOPLASMIC RETICULUM STRESS; ENZYME SYNTHESIS; FUNGAL STRAIN; GENE DOSAGE; GENE OVEREXPRESSION; GENETIC MANIPULATION; GLYCOSYLATION; HETEROLOGOUS EXPRESSION; LARGE SCALE PRODUCTION; MULTIVARIATE ANALYSIS; NONHUMAN; OXIDATIVE STRESS; PICHIA PASTORIS; PROTEIN DEGRADATION; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN SECRETION; SECRETORY PATHWAY; ASPERGILLUS NIGER; BIOSYNTHESIS; CHEMISTRY; CLASSIFICATION; ELECTROPORATION; ENZYMOLOGY; FUNGAL GENE; GENETIC TRANSFORMATION; GENETICS; METABOLISM; PHYSIOLOGICAL STRESS; PHYSIOLOGY; PICHIA; PLASMID; PROTEIN TRANSPORT; SECRETION (PROCESS);

PICHIA PASTORIS;

ASPERGILLUS NIGER; ELECTROPORATION; ENDOPLASMIC RETICULUM-ASSOCIATED DEGRADATION; FUNGAL PROTEINS; GENES, FUNGAL; GLUCOSE OXIDASE; PICHIA; PLASMIDS; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN TRANSPORT; QA-SNARE PROTEINS; RECOMBINANT FUSION PROTEINS; STRESS, PHYSIOLOGICAL; TRANSFORMATION, GENETIC;

EID: 84909983203     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2014.10.006     Document Type: Article

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