Enhancement of lipase r27RCL production in Pichia pastoris by regulating gene dosage and co-expression with chaperone protein disulfide isomerase

Enzyme and Microbial Technology

Volumn 53, Issue 6-7, 2013, Pages 438-443

  Sha, Chong ^a   Yu, Xiao Wei ^a   Lin, Nai Xin ^a   Zhang, Meng ^a   Xu, Yan ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Chaperone PDI; Gene dosage; Lipase; Pichia pastoris

Indexed keywords

CHAPERONE PDI; DISULFIDE BOND FORMATION; ENDOPLASMIC RETICULUM; EXPRESSION SYSTEM; GENE DOSAGE; PICHIA PASTORIS; PROTEIN DISULFIDE ISOMERASES; RECOMBINANT STRAINS;

CELL MEMBRANES; COVALENT BONDS; ENZYME ACTIVITY; GENE EXPRESSION; LIPASES; PROTEINS; YEAST;

GENE EXPRESSION REGULATION;

CHAPERONE; DISULFIDE; ISOMERASE; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; DISULFIDE BOND; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; ENZYME RELEASE; ENZYME SYNTHESIS; FUNGAL STRAIN; GENE DOSAGE; LABORATORY DEVICE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PROCESSING; RHIZOPUS;

PICHIA PASTORIS; RHIZOPUS MICROSPORUS VAR. CHINENSIS;

CHAPERONE PDI; GENE DOSAGE; LIPASE; PICHIA PASTORIS;

ENDOPLASMIC RETICULUM; FUNGAL PROTEINS; GENE DOSAGE; GENE EXPRESSION; GENES, FUNGAL; GENETIC ENGINEERING; LIPASE; MOLECULAR CHAPERONES; PICHIA; PROTEIN DISULFIDE-ISOMERASES; PROTEIN FOLDING; RECOMBINANT PROTEINS; RHIZOPUS;

EID: 84886384298     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2013.09.009     Document Type: Article

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