Effects of salt-induced reversible self-association on the elution behavior of a monoclonal antibody in cation exchange chromatography

Journal of Chromatography A

Volumn 1362, Issue , 2014, Pages 186-193

  Luo, Haibin ^a   Macapagal, Nathaniel ^a   Newell, Kelcy ^a   Man, Adrian ^a   Parupudi, Arun ^a   Li, Yiming ^a   Li, Yuling ^a  

^a MEDIMMUNE   (United States)

Author keywords

Cation exchange chromatography (CEX); Mobile phase modifier; Monoclonal antibody (mAb); Peak splitting; Reversible self association (RSA)

Indexed keywords

AMINO ACIDS; ASSOCIATION REACTIONS; MONOCLONAL ANTIBODIES; PHASE SEPARATION; POSITIVE IONS; SODIUM CHLORIDE;

CATIONEXCHANGE CHROMATOGRAPHY (CEX); MOBILE PHASE MODIFIERS; MONOCLONAL ANTIBODIES (MAB); PEAK-SPLITTING; SELF-ASSOCIATIONS;

CHROMATOGRAPHY;

CALCIUM CHLORIDE; HISTIDINE; IMMUNOGLOBULIN G2; MONOCLONAL ANTIBODY; PROTEIN; SODIUM CHLORIDE; CATION; IMMUNOGLOBULIN G; SOLUTION AND SOLUBILITY;

ARTICLE; CHEMICAL ANALYSIS; CHEMICAL BINDING; CHEMICAL PHENOMENA; CONCENTRATION (PARAMETERS); ELUTION; ION EXCHANGE CHROMATOGRAPHY; PH; REVERSIBLE SELF ASSOCIATION; SEPARATION TECHNIQUE; CHEMISTRY; HUMAN; ISOLATION AND PURIFICATION; PROCEDURES; PROTEIN MULTIMERIZATION; SOLUTION AND SOLUBILITY;

ANTIBODIES, MONOCLONAL; CATIONS; CHROMATOGRAPHY, ION EXCHANGE; HUMANS; IMMUNOGLOBULIN G; PROTEIN MULTIMERIZATION; SODIUM CHLORIDE; SOLUTIONS;

EID: 84908602635     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.08.048     Document Type: Article

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