Toll-like receptor expression in crypt epithelial cells, putative stem cells and intestinal myofibroblasts isolated from controls and patients with inflammatory bowel disease

Clinical and Experimental Immunology

Volumn 178, Issue 1, 2014, Pages 28-39

  Brown, M ^a,b   Hughes, K R ^a,b   Moossavi, S ^a,b   Robins, A ^a   Mahida, Y R ^a,b  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Crohn's disease; Ulcerative colitis

Indexed keywords

ADALIMUMAB; AZATHIOPRINE; CORTICOSTEROID; CYCLOSPORIN; INFLIXIMAB; MESALAZINE; METHOTREXATE; METRONIDAZOLE; TOLL LIKE RECEPTOR 2; TOLL LIKE RECEPTOR 4; TOLL LIKE RECEPTOR 5; TOLL LIKE RECEPTOR;

ADULT; ARTICLE; CELL ISOLATION; CELL SURFACE; CLINICAL ARTICLE; COLON MUCOSA; CONTROLLED STUDY; CROHN DISEASE; EPITHELIUM CELL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; INFLAMMATORY BOWEL DISEASE; INTESTINE CRYPT; INTESTINE EPITHELIUM CELL; MONOLAYER CULTURE; MYOFIBROBLAST; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEM CELL; ULCERATIVE COLITIS; AGED; BIOSYNTHESIS; CASE CONTROL STUDY; COLON; CYTOLOGY; ILEUM; INTESTINE MUCOSA; METABOLISM; MIDDLE AGED; PATHOLOGY;

ADULT; AGED; CASE-CONTROL STUDIES; COLITIS, ULCERATIVE; COLON; CROHN DISEASE; EPITHELIAL CELLS; HUMANS; ILEUM; INFLAMMATORY BOWEL DISEASES; INTESTINAL MUCOSA; MIDDLE AGED; MYOFIBROBLASTS; STEM CELLS; TOLL-LIKE RECEPTORS;

EID: 84908206016     PISSN: 00099104     EISSN: 13652249     Source Type: Journal    
DOI: 10.1111/cei.12381     Document Type: Article

References (43)

1. Khor B, Gardet A, Xavier RJ. Genetics and pathogenesis of inflammatory bowel disease. Nature 2011; 474:307-17.
2. Abraham C, Cho JH. Inflammatory bowel disease. N Engl J Med 2009; 361:2066-78.
3. Potten CS, Gandara R, Mahida YR, Loeffler M, Wright NA. The stem cells of small intestinal crypts: where are they? Cell Prolif 2009; 42:731-50.
4. Medema JP, Vermeulen L. Microenvironmental regulation of stem cells in intestinal homeostasis and cancer. Nature 2011; 474:318-26.
5. Garrett WS, Gordon JI, Glimcher LH. Homeostasis and inflammation in the intestine. Cell 2010; 140:859-70.
6. Takeda K, Akira S. Toll-like receptors in innate immunity. Int Immunol 2005; 17:1-14.
7. Rakoff-Nahoum S, Paglino J, Eslami-Varzaneh F, Edberg S, Medzhitov R. Recognition of commensal microflora by Toll-like receptors is required for intestinal homeostasis. Cell 2004; 118:229-41.
8. Fukata M, Chen A, Klepper A et al. Cox-2 is regulated by Toll-like receptor-4 (TLR4) signaling: role in proliferation and apoptosis in the intestine. Gastroenterology 2006; 131:862-77.
9. Cario E, Gerken G, Podolsky DK. Toll-like receptor 2 controls mucosal inflammation by regulating epithelial barrier function. Gastroenterology 2007; 132:1359-74.
10. Burdelya LG, Krivokrysenko VI, Tallant TC et al. An agonist of Toll-like receptor 5 has radioprotective activity in mouse and primate models. Science 2008; 320:226-30.
11. Riehl T, Cohn S, Tessner T, Schloemann S, Stenson WF. Lipopolysaccharide is radioprotective in the mouse intestine through a prostaglandin-mediated mechanism. Gastroenterology 2000; 118:1106-16.
12. Cario E. Therapeutic impact of toll-like receptors on inflammatory bowel diseases: a multiple-edged sword. Inflamm Bowel Dis 2008; 14:411-21.
13. Furrie E, Macfarlane S, Thomson G et al. Toll-like receptors-2, -3 and -4 expression patterns on human colon and their regulation by mucosal-associated bacteria. Immunology 2005; 115:565-74.
14. Fukata M, Chen A, Vamadevan AS et al. Toll-like receptor-4 promotes the development of colitis-associated colorectal tumors. Gastroenterology 2007; 133:1869-81.
15. Cario E, Podolsky DK. Differential alteration in intestinal epithelial cell expression of Toll-like receptor 3 (TLR3) and TLR4 in inflammatory bowel disease. Infect Immun 2000; 68:7010-7.
16. Hausmann M, Kiessling S, Mestermann S et al. Toll-like receptors 2 and 4 are up-regulated during intestinal inflammation. Gastroenterology 2002; 122:1987-2000.
17. Beltinger J, McKaig BC, Makh S, Stack WA, Hawkey CJ, Mahida YR. Human colonic subepithelial myofibroblasts modulate transepithelial resistance and secretory response. Am J Physiol 1999; 277:C271-9.
18. McKaig BC, Makh SS, Hawkey CJ, Podolsky DK, Mahida YR. Normal human colonic subepithelial myofibroblasts enhance epithelial migration (restitution) via TGF-beta3. Am J Physiol 1999; 276:G1087-93.
19. Hughes KR, Sablitzky F, Mahida YR. Expression profiling of Wnt family of genes in normal and inflammatory bowel disease primary human intestinal myofibroblasts and normal human colonic crypt epithelial cells. Inflamm Bowel Dis 2011; 17:213-20.
20. Kosinski C, Li VS, Chan AS et al. Gene expression patterns of human colon tops and basal crypts and BMP antagonists as intestinal stem cell niche factors. Proc Natl Acad Sci USA 2007; 104:15418-23.
21. Otte JM, Rosenberg IM, Podolsky DK. Intestinal myofibroblasts in innate immune responses of the intestine. Gastroenterology 2003; 124:1866-78.
22. Walton KL, Holt L, Sartor RB. Lipopolysaccharide activates innate immune responses in murine intestinal myofibroblasts through multiple signaling pathways. Am J Physiol Gastrointest Liver Physiol 2009; 296:G601-11.
23. Whitehead RH, Demmler K, Rockman SP, Watson NK. Clonogenic growth of epithelial cells from normal colonic mucosa from both mice and humans. Gastroenterology 1999; 117:858-65.
24. Samuel S, Walsh R, Webb J, Robins A, Potten C, Mahida YR. Characterization of putative stem cells in isolated human colonic crypt epithelial cells and their interactions with myofibroblasts. Am J Physiol Cell Physiol 2009; 296:C296-305.
25. Mahida YR, Beltinger J, Makh S et al. Adult human colonic subepithelial myofibroblasts express extracellular matrix proteins and cyclooxygenase-1 and -2. Am J Physiol 1997; 273:G1341-8.
26. Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med 1996; 183:1797-806.
27. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29:e45.
28. Suzuki M, Hisamatsu T, Podolsky DK. Gamma interferon augments the intracellular pathway for lipopolysaccharide (LPS) recognition in human intestinal epithelial cells through coordinated up-regulation of LPS uptake and expression of the intracellular Toll-like receptor 4-MD-2 complex. Infect Immun 2003; 71:3503-11.
29. Hornef MW, Frisan T, Vandewalle A, Normark S, Richter-Dahlfors A. Toll-like receptor 4 resides in the Golgi apparatus and colocalizes with internalized lipopolysaccharide in intestinal epithelial cells. J Exp Med 2002; 195:559-70.
30. Cario E, Brown D, McKee M, Lynch-Devaney K, Gerken G, Podolsky DK. Commensal-associated molecular patterns induce selective toll-like receptor-trafficking from apical membrane to cytoplasmic compartments in polarized intestinal epithelium. Am J Pathol 2002; 160:165-73.
31. Abreu MT, Arnold ET, Thomas LS et al. TLR4 and MD-2 expression is regulated by immune-mediated signals in human intestinal epithelial cells. J Biol Chem 2002; 277:20431-7.
32. Dekaney CM, Rodriguez JM, Graul MC, Henning SJ. Isolation and characterization of a putative intestinal stem cell fraction from mouse jejunum. Gastroenterology 2005; 129:1567-80.
33. Fatima S, Zhou S, Sorrentino BP. Abcg2 expression marks tissuespecific stem cells in multiple organs in a mouse progeny tracking model. Stem Cells 2012; 30:210-21.
34. Santaolalla R, Sussman DA, Ruiz JR et al. TLR4 activates the betacatenin pathway to cause intestinal neoplasia. PLOS ONE 2013; 8:e63298.
35. Neal MD, Sodhi CP, Jia H et al. Toll-like receptor 4 is expressed on intestinal stem cells and regulates their proliferation and apoptosis via the p53 up-regulated modulator of apoptosis. J Biol Chem 2012; 287:37296-308.
36. Sodhi CP, Neal MD, Siggers R et al. Intestinal epithelial Toll-like receptor 4 regulates goblet cell development and is required for necrotizing enterocolitis in mice. Gastroenterology 2012; 143:708-18.e1-5.
37. Abreu MT. Toll-like receptor signalling in the intestinal epithelium:how bacterial recognition shapes intestinal function. Nat Rev Immunol 2010; 10:131-44.
38. Fukata M, Michelsen KS, Eri R et al. Toll-like receptor-4 is required for intestinal response to epithelial injury and limiting bacterial translocation in a murine model of acute colitis. Am J Physiol Gastrointest Liver Physiol 2005; 288:G1055-65.
39. Podolsky DK, Gerken G, Eyking A, Cario E. Colitis-associated variant of TLR2 causes impaired mucosal repair because of TFF3 deficiency. Gastroenterology 2009; 137:209-20.
40. Gibson DL, Ma C, Rosenberger CM et al. Toll-like receptor 2 plays a critical role in maintaining mucosal integrity during Citrobacter rodentium-induced colitis. Cell Microbiol 2008; 10:388-403.
41. Nik AM, Reyahi A, Ponten F, Carlsson P. Foxf2 in intestinal fibroblasts reduces numbers of Lgr5(+) stem cells and adenoma formation by inhibiting Wnt signaling. Gastroenterology 2013; 144:1001-11.
42. Powell DW, Pinchuk IV, Saada JI, Chen X, Mifflin RC. Mesenchymal cells of the intestinal lamina propria. Annu Rev Physiol 2011; 73:213-37.
43. Owens BM, Simmons A. Intestinal stromal cells in mucosal immunity and homeostasis. Mucosal Immunol 2013; 6:224-34.