Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo

Journal of Experimental Medicine

Volumn 183, Issue 4, 1996, Pages 1797-1806

  Goodell, Margaret A ^a   Brose, Katja ^a,c   Paradis, Glenn ^b   Conner, A Stewart ^b,d   Mulligan, Richard C ^a,b  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d AUBURN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE MARKER;

ANIMAL CELL; ARTICLE; BONE MARROW CELL; CELL DIVISION; CELL ISOLATION; CELL MATURATION; CELL PROLIFERATION; CELL RENEWAL; DNA CONTENT; GENE REPLICATION; HEMATOPOIETIC CELL; IN VIVO STUDY; MOUSE; MULTIDRUG RESISTANCE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; ANTIGENS, LY; BENZIMIDAZOLES; BONE MARROW; BONE MARROW CELLS; BONE MARROW TRANSPLANTATION; CELL DIVISION; CELL SEPARATION; FEMALE; FLUORESCENT DYES; HEMATOPOIETIC STEM CELLS; MEMBRANE PROTEINS; MICE; MICE, INBRED C57BL; RADIATION PROTECTION; SPLEEN; STAINING AND LABELING; VERAPAMIL;

EID: 0029958876     PISSN: 00221007     EISSN: None     Source Type: Journal    
DOI: 10.1084/jem.183.4.1797     Document Type: Article

References (39)

1. Ross, E.A., N. Anderson, and H.S. Micklem. 1982. Serial depletion and regeneration of the murine hematopoietic system. Implications for hematopoietic organization and the study of cellular aging. J. Exp. Med. 155:432-444.
2. Jordan, C T., and I.R. Lemischka. 1990. Clonal and systemic analysis of long-term hematopoiesis in the mouse. Genes & Dev. 4:220-232.
3. Siminovitch, L., J.E. Till, and E.A. McCulloch. 1964. Decline in colony forming ability of marrow cells subjected to serial transplantation into irradiated mice. J. Cell. Camp. Physiol. 64:23-31.
4. Cudkowicz, G., A.C. Upton, G.M. Shearer, and W L. Hughes. 1964 Lymphocyte content and proliferadve capacity of serially transplanted mouse bone marrow, Nature (Lond.) 201:165-172.
5. Mauch, P., and S. Hellman. 1989. Loss of hematopoietic stem cell self-renewal after bone marrow transplantation. Blood. 74:872-875.
6. Harrison, D.E., M. Stone, and C.M. Astle. 1990. Effects of transplantation on the primitive immunohematopoietic stem cell. J. Exp. Med. 172:431-437.
7. Spangrude, G.J., D.M. Brooks, and D.B. Tumas. 1995. Long-term repopulation of irradiated mice with limiting numbers of purified hematopoietic stem cells: in vivo expansion of stem cell phenotype but not function. Blood. 85:1006-1016.
8. Van Zant, G., B.P. Holland, P.W. Eldrige, and J.J. Chen. 1990. Genotype-restricted growth and aging patterns in hematopoietic stem cell populations of allophenic mice. J. Exp. Med. 171:1547-1565.
9. Gray, J.W., and Z. Darzynkiewicz. 1986. Techniques in Cell Cycle Analysis. Humana Press, Clifton, NJ. 407 pp.
10. Watson. J.V., A. Nakelf, S.H. Chambers, and P.J. Smith. 1985. Flow cytometric fluorescence emission spectrum analysis of Hoechst-33342-stamed DNA in chicken thymocytes. Cytometry. 6:310-315.
11. Pallavicini, M.G.. L.J. Summers, P.N. Dean, and J.W. Gray. 1985. Enrichment of murine hemopoietic clonogenic cells by multivanate analyses and sorting. Exp. Hematol. 13:1173-1181.
12. Spangrude, G.J., S. Heimfeld, and I.L. Weissman. 1988. Purification and characterization of mouse hematopoietic stem cells. Science (Wash. DC). 241:58-62.
13. + cells are the only stem cells in C57BL/Ka-Thy-1.1 bone marrow. J. Exp. Med. 175:175-184.
14. + cells. Exp. Hematol. 20:1309-1315.
15. Harrison, D.E. 1980. Competitive repopulation: a new assay for long-term stem cell function. Blood. 55:77-81.
16. Harrison, D.E., C.M. Astle, and C. Lemer. 1988. Number and continuous proliferative pattern of transplanted primitive immunohematopoietic stem cells. Proc. Natl. Acad. Sci. USA. 85:822-826.
17. Scheid, M.P., and D. Triglia. 1979. Further description of the Ly-5 system. Immunogenetics. 9:423-433.
18. Spangrude, G.J., and D.M. Brooks. 1993. Mouse strain variability in the expression of the hematopoietic stem cell antigen Ly-6A/E by bone marrow cells. Blood. 82:3327-3332.
19. Fleming, W.H., E.J. Alpern, N. Uchida, K. Ikuta, G.J. Spangrude, and I.L. Weissman. 1993. Functional heterogeneity is associated with the cell cycle status of murine hematopoietic stem cells. J. Cell biol. 122:897-902.
20. Spangrude, G.J. 1991. Hematopoietic stem-cell differentiation. Curr. Opin. Immunol. 3:171-178.
21. Visser, J.W., J.G. Bauman, A.H. Mulder, J.F. Eliason, and A.M. de Leeuw. 1984. Isolation of murine pluripotent hemopoietic stem cells. J. Exp. Med. 159:1576-1590.
22. Szilvassy, S.J., P.M. Lansdorp, R.K. Humphries, A.C. Eaves, and C.J. Eaves. 1989. Isolation in a single step of a highly enriched murine hematopoietic stem cell population with competitive long-term repopulating ability. Blood. 74:930-939.
23. Jones, R.J., J.E. Wagner, P. Celano, M.S. Zicha, and S.J. Sharkis. 1990. Separation of pluripotent haematopoietic stem cells from spleen colony-forming cells. Nature (Lond). 347: 188-189.
24. Bertoncello, I., G.S. Hodgson, and T.R. Bradley. 1985. Multiparameter analysis of transplantable hemopoietic stem cells: I. The separation and enrichment of stem cells homing to marrow and spleen on the basis of rhodamine-123 fluorescence. Exp. Hematol 13:999-1006.
25. Fleming, W.H., E.J. Alpern, N. Uchida, K. Ikuta, G.J. Spangrude, and I.L. Weissman. 1993. Functional heterogeneity is associated with the cell cycle status of murine hematopoietic stem cells. J. Cell biol. 122:897-902.
26. Spangrude, G.J., and R. Scollay. 1990. A simplified method for enrichment of mouse hematopoietic stem cells. Exp. Hematol. 18:920-926.
27. Neben, S., W.J. Redfearn, M. Parra, G. Brecher, and M.G. Pallavicini. 1991. Short- and long-term repopulation of Iethally irradiated mice by bone marrow stem cells enriched on the basis of light scatter and Hoechst 33342 fluorescence. Exp. Hematol. 19:958-967.
28. McAlister, I., N.S. Wolf, M.E. Pietrzyk, P.S. Rabinovitch, G. Priestley, and B. Jaeger. 1990. Transplantation of hematoporetic stem cells obtained by a combined dye method fractionation of murine bone marrow. Blood. 75:1240-1246.
29. Wolf, N.S., A. Kone, G.V. Priestley, and S.H. Bartelmez. 1993. In vivo and in vitro characterization of long-term repopulating primitive hematopoietic cells isolated by sequenctional Hoechst 33342-rhodamine 123 FACS selection. Exp. Hematol. 21:614-622.
30. Ellwart, J.W., and P. Dormer. 1990. Vitality measurement using spectrum shift in Hoechst 33342 stained cells. Cytometry. 11:239-243.
31. Chaudhary, P.M., and I.B. Roninson. 1991. Expression and activity of P-glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells. Cell. 66:85-94.
32. Drach, D., S Zhao, J. Drach, R. Mahadevia, C. Gattringer, H. Huber, and M. Andreeff. 1992. Subpopulations of normal peripheral blood and bone marrow cells express a functional multidrug resistant phenotype. Blood. 80:2729-2734.
33. Spangrude, G.J., and G.R. Johnson. 1990. Resting and activated subsets of mouse multipotent hematopoietic stem cells. Proc. Natl. Acad. Sci. USA. 87:7433-7437.
34. + hemopoietic stem cells. J. Exp. Med. 175:1443-1447.
35. Ploemacher, R.E., and R.H. Brons. 1989. Separation of CFU-S from primitive cells responsible for reconstitution of the bone marrow hemopoietic stem cell compartment following irradiation: evidence for a pre-CFU-S cell. Exp. Hematol. 17:263-266.
36. Civin, C.I., L.C. Strauss, C. Brovall, M.J. Fackler, J.F. Schwartz, and J.H. Shaper. 1984. Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J. Immunol. 133:157-165.
37. - cells that lack detectable T cell, B cell, and myeloid cell antigens produce multiple colony-forming cells when cultured with marrow stromal cells. J. Exp. Med. 172:355-358.
38. Tindle, R.W., R.A. Nichols, L. Chan, D. Campana, D. Catovsky, and G.D. Birnie. 1985. A novel monoclonal antibody BI-3C5 recognises myeloblasts and non-B non-T lymphoblasts in acute leukaemias and CGL blast crises, and reacts with immature cells in normal bone marrow. Leuk. Res. 9:1-9.
39. Watt, S.M., K. Karhi, K. Gatter, A.J. Furley, F.E. Katz, L.E. Healy, L.J. Altass, N.J. Bradley, D.R. Sutherland, R. Levinsky et al. 1987. Distribution and epitope analysis of the cell membrane glycoprotem (HPCA-1) associated with human hemopoietic progenitor cells. Leukemia. 1:417-126.