Gene expression measurements normalized to cell number reveal large scale differences due to cell size changes, transcriptional amplification and transcriptional repression in CHO cells

Journal of Biotechnology

Volumn 189, Issue , 2014, Pages 58-69

  Fomina Yadlin, Dina ^a   Du, Zhimei ^a   McGrew, Jeffrey T ^a  

^a AMGEN INC   (United States)

Author keywords

Cellular RNA content; Chinese hamster ovary; Gene expression; Specific productivity; Synthetic RNA spike in controls

Indexed keywords

CELL NUMBER; CELL SIZE; CELLULAR RNA; CHINESE HAMSTER OVARY; EXPRESSION MEASUREMENTS; IN-CONTROL; SPECIFIC PRODUCTIVITY; TRANSCRIPTIONAL REPRESSION;

GENE EXPRESSION;

MAMMALIAN TARGET OF RAPAMYCIN INHIBITOR; RECOMBINANT PROTEIN; RNA;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL SIZE; CHO CELL LINE; CONTROLLED STUDY; FEMALE; GENE AMPLIFICATION; GENE EXPRESSION; GENE REPRESSION; GENETIC TRANSCRIPTION; NONHUMAN; OSMOLARITY; PRODUCTIVITY; RNA ANALYSIS; ANIMALS; CHO CELLS; CRICETULUS; GENETICS;

ANIMALS; CELL SIZE; CHO CELLS; CRICETULUS; GENE EXPRESSION; RNA;

EID: 84908192381     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.08.037     Document Type: Article

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