Transcriptomic responses to sodium chloride-induced osmotic stress: A study of industrial fed-batch CHO cell cultures

Biotechnology Progress

Volumn 26, Issue 4, 2010, Pages 1104-1115

  Shen, Duan ^a   Kiehl, Thomas R ^a   Khattak, Sarwat F ^b   Li, Zheng Jian ^b   He, Aiqing ^c   Kayne, Paul S ^c   Patel, Vishal ^c   Neuhaus, Isaac M ^c   Sharfstein, Susan T ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^b BRISTOL MYERS SQUIBB COMPANY   (United States)

^c BRISTOL MYERS SQUIBB   (United States)

Author keywords

Chinese hamster ovary cell; Hybridoma; Microarray; Osmotic stress; Transcriptome

Indexed keywords

BATCH CULTURE; CAPACITY LIMITATION; CELL LINES; CELLULAR GROWTH RATE; CHINESE HAMSTER OVARY CELLS; CHO CELL; COST-OF-GOODS; FED BATCHES; FED-BATCH CULTURES; GENE LISTS; HYBRIDOMA; HYPEROSMOLARITY; HYPEROSMOTIC STRESS; INCREASED PRODUCTIVITY; MAMMALIAN CELL LINES; MAMMALIAN CELLS; METABOLIC CHANGES; ONLINE DATABASE; OSMOLARITY; OSMOTIC STRESS; PROTEIN THERAPEUTICS; REGULATED GENES; SPECIFIC PRODUCTIVITY; TRANSCRIPTION PROFILES; TRANSCRIPTIONAL RESPONSE; TRANSCRIPTOMES; VIABLE CELLS;

ANIMAL CELL CULTURE; BATCH CELL CULTURE; CELLS; FUNCTIONAL GROUPS; MAMMALS; MICROARRAYS; MONOCLONAL ANTIBODIES; OSMOSIS; PRODUCTIVITY; SODIUM; SODIUM CHLORIDE; TRANSCRIPTION;

CELL CULTURE;

SODIUM CHLORIDE;

ANIMAL; ARTICLE; CELL CYCLE; CELL DEATH; CELL DIFFERENTIATION; CHO CELL; CRICETULUS; DRUG EFFECT; GENE EXPRESSION PROFILING; HAMSTER; METHODOLOGY; MOUSE; OSMOTIC PRESSURE; SIGNAL TRANSDUCTION;

ANIMALS; CELL CYCLE; CELL DEATH; CELL DIFFERENTIATION; CHO CELLS; CRICETINAE; CRICETULUS; GENE EXPRESSION PROFILING; MICE; OSMOTIC PRESSURE; SIGNAL TRANSDUCTION; SODIUM CHLORIDE;

BACTERIA (MICROORGANISMS); CRICETULUS GRISEUS; MAMMALIA; MURINAE;

EID: 77957123642     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.398     Document Type: Article

References (46)

1. Wurm FM. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol. 2004;22:1393-1398.
2. Bibila TA, Robinson DK. In pursuit of the optimal fed-batch process for monoclonal antibody production. Biotechnol Prog. 1995;11:1-13.
3. Bibila TA, Ranucci CS, Glazomitsky K, Buckland BC, Aunins JG. Monoclonal antibody process development using medium concentrates. Biotechnol Prog. 1994;10:87-96.
4. Ryu JS, Lee GM. Application of hypoosmolar medium to fedbatch culture of hybridoma cells for improvement of culture longevity. Biotechnol Bioeng. 1999;62:120-123.
5. Mikama T, Makishima F, Suzuki E. Enhancing in vitro antibody production rate per cell by applying mouse peritoneal factors. Biotechnol Lett. 1991;13:255-260.
6. Suzuki E, Ollis DF. Enhanced antibody production at slowed growth rates: experimental demonstration and a simple structured model. Biotechnol Prog. 1990;6:231-236.
7. Pendse GJ, Bailey JE. Effects of growth factors on cell proliferation and monoclonal antibody production of batch hybridoma cultures. Biotechnol Let. 1990;7:487-492.
8. Dalili M, Ollis DF. The influence of cyclic nucleotides on hybridoma growth and monoclonal antibody production. Biotechnol Lett. 1988;10:781-786.
9. Sureshkumar GK, Mutharasan R. The influence of temperature on a mouse-mouse hybridoma growth and monoclonal antibody production. Biotechnol Bioeng. 1991;37:292-295.
10. Fox SR, Patel UA, Yap MG, Wang DI. Maximizing interferongamma production by Chinese hamster ovary cells through temperature shift optimization: experimental and modeling. Biotechnol Bioeng. 2004;85:177-184.
11. Reuveney S, Velez D, Miller L, Macmillan JD. Factors affecting cell growth and monoclonal antibody production in stirred reactors. J. Immunol. Method. 1986;86:53-59.
12. Ozturk SS, Palsson BO. Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production. Biotechnol Bioeng. 1991;37:989-993.
13. Kim NS, Lee GM. Response of recombinant Chinese hamster ovary cells to hyperosmotic pressure: effect of Bcl-2 overexpression. J Biotechnol. 2002;95:237-248.
14. Wu M-H, Dimopoulos G, Mantalaris A, Varley J. The effect of hyperosmotic pressure on antibody production and gene expression in the GS-NS0 cell line. Biotechnol Appl Biochem. 2004;40:41-46.
15. Ryu JS, Kim TK, Chung JY, Lee GM. Osmoprotective effect of glycine betaine on foreign protein production in hyperosmotic recombinant Chinese hamster ovary cell cultures differs among cell lines. Biotechnol Bioeng. 2000;70:167-175.
16. Lee MS, Kim KW, Kim YH, Lee GM. Proteome analysis of antibody-expressing CHO cells in response to hyperosmotic pressure. Biotech Prog. 2003;19:1734-1741.
17. Lee GM, Park SY. Enhanced specific antibody productivity of hybridomas resulting from hyperosmotic stress is cell line-specific. Biotechnol Lett. 1995;17:145-150.
18. Reddy S, Miller WM. Effects of abrupt and gradual osmoticstress on antibody-production and content in hybridoma cells that differ in production kinetics. Biotechnol Prog. 1994;10: 165-173.
19. deZengotita VM, Schmelzer AE, Miller WM. Characterization of hybridoma cell responses to elevated pCO(2) and osmolality: intracellular pH, cell size, apoptosis, and metabolism. Biotechnol Bioeng. 2002;77:369-380.
20. Oh SKW, Chua FKF, Choo ABH. Intracellular responses of productive hybridomas subjected to high osmotic pressure. Biotechnol Bioeng. 1995;46:525-535.
21. Lee MS, Lee GM. Hyperosmotic pressure enhances immunoglobulin transcription rates and secretion rates of KR12H-2 transfectoma. Biotechnol Bioeng. 2000;68:260-268.
22. Sun Z, Zhou R, Liang S, McNeeley KM, Sharfstein ST. Hyperosmotic stress in murine hybridoma cells: effects on antibody transcription, translation, posttranslational processing, and the cell cycle. Biotechnol Prog. 2004;20:576-589.
23. Korke R, Gatti MdL, Lau ALY, Lim JWE, Seow TK, Chung MCM, Hu W-S. Large scale gene expression profiling of metabolic shift of mammalian cells in culture. J Biotechnol. 2004;107:1-17.
24. Yee JC, Gatti MD, Philp RJ, Yap M, Hu WS. Genomic and proteomic exploration of CHO and hybridoma cells under sodium butyrate treatment. Biotechnol Bioeng. 2008;99:1186-1204.
25. Yee JC, Gerdtzen ZP, Hu W-S. Comparative transcriptome analysis to unveil genes affecting recombinant protein productivity in mammalian cells. Biotechnol Bioeng. 2009;102:246-263.
26. Swiderek H, Al-Rubeai M. Functional genome-wide analysis of antibody producing NS0 cell line cultivated at different temperatures. Biotechnol Bioeng. 2007;98:616-630.
27. Shen D, Sharfstein ST. Genome-wide analysis of the transcriptional response of murine hybridomas to osmotic shock. Biotechnol Bioeng. 2006;93:132-145.
28. Wlaschin KF, Nissom PM, Gatti MdL, Ong PF, Arleen S, Tan KS, Rink A, Cham B, Wong KTK, Yap M, Hu WS. EST sequencing for gene discovery in Chinese hamster ovary cells. Biotechnol Bioeng. 2005;91:592-606.
29. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215:403-410.
30. Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U, Speed TP. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostat. 2003;4:249-264.
31. Dalili M, Ollis DF. A flow-cytometric analysis of hybridoma growth and monoclonal antibody production. Biotechnol Bioeng. 1990;36:64-73.
32. McKinney KL, Dilwith R, Belfort G. Manipulation of heterogeneous hybridoma cultures for overproduction of monoclonal antibodies. Biotechnol Prog. 1991;7:445-454.
33. Hayter PM, Kirkby NF, Spier RE. Relationship between hybridoma growth and monoclonal antibody production. Enzyme Microb Technol. 1992;14:454-461.
34. Kromenaker SJ, Srienc F. Cell cycle kinetics of the accumulation of heavy and light chain immunoglobulin proteins in a mouse hybridoma cell line. Cytotechnology. 1994;14:205-218.
35. Park SH, Ryu DD. Cell cycle kinetics and monoclonal antibody productivity of hybridoma cells during perfusion culture. Biotechnol Bioeng. 1994;44:361-367.
36. Bibila TA, Flickinger MC. Use of a structured kinetic model of antibody synthesis and secretion for optimization of antibody production systems. I. Steady-state analysis. Biotechnol Bioeng. 1992;39:251-261.
37. Bibila TA, Flickinger MC. Use of a structured kinetic model of antibody synthesis and secretion for optimization of antibody production systems. II. Transient analysis. Biotechnol Bioeng. 1992;39:262-272.
38. Bibila T, Flickinger MC. A structured model for monoclonal antibody synthesis in exponentially growing and stationary phase hybridoma cells. Biotechnol Bioeng. 1991;37:210-226.
39. Bibila TA, Flickinger MC. A model of interorganelle monoclonal antibody transport and secretion in mouse hybridoma cells. Biotechnol Bioeng. 1991;38:767-780.
40. Kashiwagi H, Yamazaki K, Takekuma Y, Ganapathy V, Sugawara M. Regulatory mechanisms of SNAT2, an amino acid transporter, in L6 rat skeletal muscle cells by insulin, osmotic shock and amino acid deprivation. Amino Acids. 2009;36:219-230.
41. Maallem S, Mutin M, González-González IM, Zafra F, Tappaz ML. Selective tonicity-induced expression of the neutral aminoacid transporter SNAT2 in oligodendrocytes in rat brain following systemic hypertonicity. Neuroscience. 2008;153:95-107.
42. Burg MB, Ferraris JD, Dmitrieva NI. Cellular response to hyperosmotic stresses. Physiol Rev. 2007;87:1441-1474.
43. Norat T, Bowman R, Luben R, Welch A, Khaw KT, Wareham N, Bingham S. Blood pressure and interactions between the angiotensin polymorphism AGT M235T and sodium intake: a cross-sectional population study. Am J Clin Nutr. 2008;88:392-397.
44. De Leon Gatti M, Wlaschin KF, Nissom PM, Yap M, Hu W-S. Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment. J Biosci Bioeng. 2007;103:82-91.
45. Baik JY, Lee MS, An SR, Yoon SK, Joo EJ, Kim YH, Park HW, Lee GM. Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin. Biotechnol Bioeng. 2006;93:361-371.
46. Wu MK, Cohen DE. Altered hepatic cholesterol metabolism compensates for disruption of phosphatidylcholine transfer protein in mice. Am J Physiol Gastrointest Liver Physiol. 2005;289:G456-G461.