Rapamycin treatment inhibits CHO cell death in a serum-free suspension culture by autophagy induction

Biotechnology and Bioengineering

Volumn 109, Issue 12, 2012, Pages 3093-3102

  Lee, Jae Seong ^a   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Apoptosis; Autophagy; Rapamycin; RCHO cells

Indexed keywords

ANTIBODY CONCENTRATION; APOPTOSIS INDUCTION; AUTOPHAGY; BAFILOMYCIN; CELL LINES; CELL VIABILITY; CHEMICAL ACTIVATORS; CHINESE HAMSTER OVARY CELLS; CHO CELL; ENGINEERING FIELDS; EVEROLIMUS; EXPRESSION LEVELS; HOST CELLS; IN-VITRO; RAPAMYCIN; RAPAMYCIN TREATMENT; SUSPENSION CULTURES;

ANTIBIOTICS; ANTIBODIES; BODY FLUIDS; CELL CULTURE;

CELL DEATH;

COMPLEMENTARY DNA; EVEROLIMUS; MAMMALIAN TARGET OF RAPAMYCIN INHIBITOR; RAPAMYCIN; SMALL INTERFERING RNA; TACROLIMUS;

ANIMAL CELL; APOPTOSIS; ARTICLE; AUTOPHAGY; BIOENGINEERING; CELL DEATH; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; DNA SEQUENCE; DRUG EFFECT; DRUG TARGETING; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; RAT; SEQUENCE HOMOLOGY; SUSPENSION CELL CULTURE; WESTERN BLOTTING;

ANALYSIS OF VARIANCE; ANIMALS; ANTIBODIES; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; AUTOPHAGY; BIOENGINEERING; BLOTTING, WESTERN; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA, SERUM-FREE; SIROLIMUS; TOR SERINE-THREONINE KINASES;

CRICETULUS GRISEUS;

EID: 84868140882     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24567     Document Type: Article

References (35)

1. Balcarcel RR, Stephanopoulos G. 2001. Rapamycin reduces hybridoma cell death and enhances monoclonal antibody production. Biotechnol Bioeng 76(1): 1-10.
2. Blommaart EF, Luiken JJ, Blommaart PJ, van Woerkom GM, Meijer AJ. 1995. Phosphorylation of ribosomal protein S6 is inhibitory for autophagy in isolated rat hepatocytes. J Biol Chem 270(5): 2320-2326.
3. Chang YY, Juhasz G, Goraksha-Hicks P, Arsham AM, Mallin DR, Muller LK, Neufeld TP. 2009. Nutrient-dependent regulation of autophagy through the target of rapamycin pathway. Biochem Soc Trans 37(Pt 1): 232-236.
4. Ciechomska IA, Goemans GC, Skepper JN, Tolkovsky AM. 2009. Bcl-2 complexed with Beclin-1 maintains full anti-apoptotic function. Oncogene 28(21): 2128-2141.
5. Creagh EM, Conroy H, Martin SJ. 2003. Caspase-activation pathways in apoptosis and immunity. Immunol Rev 193: 10-21.
6. Dreesen IA, Fussenegger M. 2011. Ectopic expression of human mTOR increases viability, robustness, cell size, proliferation, and antibody production of Chinese hamster ovary cells. Biotechnol Bioeng 108(4): 853-866.
7. Easton JB, Houghton PJ. 2006. mTOR and cancer therapy. Oncogene 25(48): 6436-6446.
8. Gao P, Bauvy C, Souquere S, Tonelli G, Liu L, Zhu Y, Qiao Z, Bakula D, Proikas-Cezanne T, Pierron G, Codogno P, Chen Q, Mehrpour M. 2010. The Bcl-2 homology domain 3 mimetic gossypol induces both Beclin 1-dependent and Beclin 1-independent cytoprotective autophagy in cancer cells. J Biol Chem 285(33): 25570-25581.
9. Han YK, Ha TK, Lee SJ, Lee JS, Lee GM. 2011. Autophagy and apoptosis of recombinant Chinese hamster ovary cells during fed-batch culture: Effect of nutrient supplementation. Biotechnol Bioeng 108(9): 2182-2192.
10. Huang S, Bjornsti MA, Houghton PJ. 2003. Rapamycins: Mechanism of action and cellular resistance. Cancer Biol Ther 2(3): 222-232.
11. Hwang SO, Lee GM. 2008a. Autophagy and apoptosis in Chinese hamster ovary cell culture. Autophagy 4(1): 70-72.
12. Hwang SO, Lee GM. 2008b. Nutrient deprivation induces autophagy as well as apoptosis in Chinese hamster ovary cell culture. Biotechnol Bioeng 99(3): 678-685.
13. Hwang SO, Lee GM. 2009. Effect of Akt overexpression on programmed cell death in antibody-producing Chinese hamster ovary cells. J Biotechnol 139(1): 89-94.
14. Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y, Yoshimori T. 2000. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J 19(21): 5720-5728.
15. Kang R, Zeh HJ, Lotze MT, Tang D. 2011. The Beclin 1 network regulates autophagy and Apoptosis. Cell Death Differ 18(4): 571-580.
16. Kim SJ, Kim NS, Ryu CJ, Hong HJ, Lee GM. 1998. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol Bioeng 58(1): 73-84.
17. L overexpression on apoptosis and autophagy in recombinant Chinese hamster ovary cells under nutrient-deprived condition. Biotechnol Bioeng 103(4): 757-766.
18. Klionsky DJ, Abeliovich H, Agostinis P, Agrawal DK, Aliev G, Askew DS, Baba M, Baehrecke EH, Bahr BA, Ballabio A, Bamber BA, Bassham DC, Bergamini E, Bi X, Biard-Piechaczyk M, Blum JS, Bredesen DE, Brodsky JL, Brumell JH, Brunk UT, Bursch W, Camougrand N, Cebollero E, Cecconi F, Chen Y, Chin LS, Choi A, Chu CT, Chung J, Clarke PG, Clark RS, Clarke SG, Clave C, Cleveland JL, Codogno P, Colombo MI, Coto-Montes A, Cregg JM, Cuervo AM, Debnath J, Demarchi F, Dennis PB, Dennis PA, Deretic V, Devenish RJ, Di Sano F, Dice JF, Difiglia M, Dinesh-Kumar S, Distelhorst CW, Djavaheri-Mergny M, Dorsey FC, Droge W, Dron M, Dunn WA Jr, Duszenko M, Eissa NT, Elazar Z, Esclatine A, Eskelinen EL, Fesus L, Finley KD, Fuentes JM, Fueyo J, Fujisaki K, Galliot B, Gao FB, Gewirtz DA, Gibson SB, Gohla A, Goldberg AL, Gonzalez R, Gonzalez-Estevez C, Gorski S, Gottlieb RA, Haussinger D, He YW, Heidenreich K, Hill JA, Hoyer-Hansen M, Hu X, Huang WP, Iwasaki A, Jaattela M, Jackson WT, Jiang X, Jin S, Johansen T, Jung JU, Kadowaki M, Kang C, Kelekar A, Kessel DH, Kiel JA, Kim HP, Kimchi A, Kinsella TJ, Kiselyov K, Kitamoto K, Knecht E, Komatsu M, Kominami E, Kondo S, Kovacs AL, Kroemer G, Kuan CY, Kumar R, Kundu M, Landry J, Laporte M, Le W, Lei HY, Lenardo MJ, Levine B, Lieberman A, Lim KL, Lin FC, Liou W, Liu LF, Lopez-Berestein G, Lopez-Otin C, Lu B, Macleod KF, Malorni W, Martinet W, Matsuoka K, Mautner J, Meijer AJ, Melendez A, Michels P, Miotto G, Mistiaen WP, Mizushima N, Mograbi B, Monastyrska I, Moore MN, Moreira PI, Moriyasu Y, Motyl T, Munz C, Murphy LO, Naqvi NI, Neufeld TP, Nishino I, Nixon RA, Noda T, Nurnberg B, Ogawa M, Oleinick NL, Olsen LJ, Ozpolat B, Paglin S, Palmer GE, Papassideri I, Parkes M, Perlmutter DH, Perry G, Piacentini M, Pinkas-Kramarski R, Prescott M, Proikas-Cezanne T, Raben N, Rami A, Reggiori F, Rohrer B, Rubinsztein DC, Ryan KM, Sadoshima J, Sakagami H, Sakai Y, Sandri M, Sasakawa C, Sass M, Schneider C, Seglen PO, Seleverstov O, Settleman J, Shacka JJ, Shapiro IM, Sibirny A, Silva-Zacarin EC, Simon HU, Simone C, Simonsen A, Smith MA, Spanel-Borowski K, Srinivas V, Steeves M, Stenmark H, Stromhaug PE, Subauste CS, Sugimoto S, Sulzer D, Suzuki T, Swanson MS, Tabas I, Takeshita F, Talbot NJ, Talloczy Z, Tanaka K, Tanida I, Taylor GS, Taylor JP, Terman A, Tettamanti G, Thompson CB, Thumm M, Tolkovsky AM, Tooze SA, Truant R, Tumanovska LV, Uchiyama Y, Ueno T, Uzcategui NL, van der Klei I, Vaquero EC, Vellai T, Vogel MW, Wang HG, Webster P, Wiley JW, Xi Z, Xiao G, Yahalom J, Yang JM, Yap G, Yin XM, Yoshimori T, Yu L, Yue Z, Yuzaki M, Zabirnyk O, Zheng X, Zhu X, Deter RL. 2008. Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 4(2): 151-175.
19. Levine B, Yuan J. 2005. Autophagy in cell death: An innocent convict? J Clin Invest 115(10): 2679-2688.
20. Maiuri MC, Zalckvar E, Kimchi A, Kroemer G. 2007. Self-eating and self-killing: Crosstalk between autophagy and apoptosis. Nat Rev Mol Cell Biol 8(9): 741-752.
21. Mizushima N. 2010. The role of the Atg1/ULK1 complex in autophagy regulation. Curr Opin Cell Biol 22(2): 132-139.
22. Oliver FJ, de la Rubia G, Rolli V, Ruiz-Ruiz MC, de Murcia G, Murcia JM. 1998. Importance of poly(ADP-ribose) polymerase and its cleavage in apoptosis. Lesson from an uncleavable mutant. J Biol Chem 273(50): 33533-33539.
23. Pattingre S, Tassa A, Qu X, Garuti R, Liang XH, Mizushima N, Packer M, Schneider MD, Levine B. 2005. Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy. Cell 122(6): 927-939.
24. Pattingre S, Espert L, Biard-Piechaczyk M, Codogno P. 2008. Regulation of macroautophagy by mTOR and Beclin 1 complexes. Biochimie 90(2): 313-323.
25. Proikas-Cezanne T, Codogno P. 2011. Beclin 1 or not Beclin 1. Autophagy 7(7): 671-672.
26. Raught B, Gingras AC, Sonenberg N. 2001. The target of rapamycin (TOR) proteins. Proc Natl Acad Sci USA 98(13): 7037-7044.
27. Ravikumar B, Berger Z, Vacher C, O'Kane CJ, Rubinsztein DC. 2006. Rapamycin pre-treatment protects against apoptosis. Hum Mol Genet 15(7): 1209-1216.
28. Rubinsztein DC, Gestwicki JE, Murphy LO, Klionsky DJ. 2007. Potential therapeutic applications of autophagy. Nat Rev Drug Discov 6(4): 304-312.
29. Scarlatti F, Maffei R, Beau I, Codogno P, Ghidoni R. 2008. Role of non-canonical Beclin 1-independent autophagy in cell death induced by resveratrol in human breast cancer cells. Cell Death Differ 15(8): 1318-1329.
30. Schreiber SL. 1991. Chemistry and biology of the immunophilins and their immunosuppressive ligands. Science 251(4991): 283-287.
31. Scott PH, Brunn GJ, Kohn AD, Roth RA, Lawrence JC, Jr. 1998. Evidence of insulin-stimulated phosphorylation and activation of the mammalian target of rapamycin mediated by a protein kinase B signaling pathway. Proc Natl Acad Sci USA 95(13): 7772-7777.
32. Thoreen CC, Kang SA, Chang JW, Liu Q, Zhang J, Gao Y, Reichling LJ, Sim T, Sabatini DM, Gray NS. 2009. An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1. J Biol Chem 284(12): 8023-8032.
33. Vignot S, Faivre S, Aguirre D, Raymond E. 2005. mTOR-targeted therapy of cancer with rapamycin derivatives. Ann Oncol 16(4): 525-537.
34. Wiederrecht G, Lam E, Hung S, Martin M, Sigal N. 1993. The mechanism of action of FK-506 and cyclosporin A. Ann N Y Acad Sci 696: 9-19.
35. Zustiak MP, Pollack JK, Marten MR, Betenbaugh MJ. 2008. Feast or famine: Autophagy control and engineering in eukaryotic cell culture. Curr Opin Biotechnol 19(5): 518-526.