Sensitive detection of proteasomal activation using the Deg-On mammalian synthetic gene circuit

Nature Communications

Volumn 5, Issue , 2014, Pages

  Zhao, Wenting ^a   Bonem, Matthew ^a   McWhite, Claire ^b   Silberg, Jonathan J ^b   Segatori, Laura ^a,b  

^a Rice University   (United States)

^b RICE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BENZYLOXYCARBONYLLEUCYLLEUCYLLEUCINAL; GREEN FLUORESCENT PROTEIN; LACTACYSTIN; MESSENGER RNA; PROTEASOME; SYNTHETIC DNA; TETRACYCLINE; BENZYLOXYCARBONYLLEUCYL-LEUCYL-LEUCINE ALDEHYDE; ESCHERICHIA COLI PROTEIN; LEUPEPTIN; PROTEIN SYNTHESIS INHIBITOR; TETR(B) PROTEIN, E COLI; UBIQUITIN PROTEIN LIGASE;

ANTIBIOTICS; DETECTION METHOD; GENE EXPRESSION; GENETIC ENGINEERING; INHIBITOR; MAMMAL; PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ANALYSIS; ENZYME DEGRADATION; FEEDBACK SYSTEM; FEMALE; FLOW CYTOMETRY; GENETIC TRANSCRIPTION; HALF LIFE TIME; HELA CELL LINE; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; NONHUMAN; PROMOTER REGION; PROTEIN AGGREGATION; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEADY STATE; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION; TRANSPOSON; WESTERN BLOTTING; GENE REGULATORY NETWORK; SYSTEMS BIOLOGY;

MAMMALIA;

ESCHERICHIA COLI PROTEINS; GENE REGULATORY NETWORKS; GENES, SYNTHETIC; HELA CELLS; HUMANS; LEUPEPTINS; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEIN SYNTHESIS INHIBITORS; SYSTEMS BIOLOGY; TETRACYCLINE; UBIQUITIN-PROTEIN LIGASE COMPLEXES;

EID: 84908180743     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms4612     Document Type: Article

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