Anion inhibition study of the β-class carbonic anhydrase (PgiCAb) from the oral pathogen Porphyromonas gingivalis

Bioorganic and Medicinal Chemistry Letters

Volumn 24, Issue 18, 2014, Pages 4402-4406

  Vullo, Daniela ^a   Del Prete, Sonia ^b   Osman, Sameh M ^c   Scozzafava, Andrea ^a   Alothman, Zeid ^c   Supuran, Claudiu T ^a   Capasso, Clemente ^b  

^a UNIVERSITY OF FLORENCE   (Italy)

^b INSTITUTE OF PROTEIN BIOCHEMISTRY   (Italy)

^c KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

Anion inhibitor; Carbonic anhydrase; Porphyromonas gingivalis; Class enzyme

Indexed keywords

ANION; AZIDE; BENZENEBORONIC ACID; BETA CLASS CARBONATE DEHYDRATASE; CARBON DIOXIDE; CARBONATE DEHYDRATASE; CARBONATE DEHYDRATASE INHIBITOR; CYANIC ACID; CYANIDE; DIETHYLDITHIOCARBAMIC ACID; HYDROGEN SULFIDE; SULFANILAMIDE; SULFONAMIDE; THIOCYANATE; UNCLASSIFIED DRUG; ISOENZYME;

ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME INHIBITION; HYDRATION; INHIBITION CONSTANT; NONHUMAN; PERIODONTITIS; PORPHYROMONAS GINGIVALIS; AMINO ACID SEQUENCE; ANTAGONISTS AND INHIBITORS; CHEMICAL STRUCTURE; CHEMISTRY; DOSE RESPONSE; ENZYMOLOGY; HUMAN; METABOLISM; MOLECULAR GENETICS; SEQUENCE ALIGNMENT; STRUCTURE ACTIVITY RELATION; SYNTHESIS;

AMINO ACID SEQUENCE; ANIONS; CARBONIC ANHYDRASE INHIBITORS; CARBONIC ANHYDRASES; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; ISOENZYMES; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; PORPHYROMONAS GINGIVALIS; SEQUENCE ALIGNMENT; STRUCTURE-ACTIVITY RELATIONSHIP; SULFONAMIDES;

EID: 84906967985     PISSN: 0960894X     EISSN: 14643405     Source Type: Journal    
DOI: 10.1016/j.bmcl.2014.08.014     Document Type: Article

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90. R.G. Khalifah J. Biol. Chem. 246 1971 2561 An Applied Photophysics stopped-flow instrument has been used for assaying the CA catalyzed CO2hydration activity. Phenol red (at a concentration of 0.2 mM) has been used as indicator, working at the absorbance maximum of 557 nm, with 10-20 mM Hepes (pH 7.5) as buffer, and 20 mM Na2SO4or 20 mM NaBF4for maintaining constant the ionic strength, following the initial rates of the CA-catalyzed CO2hydration reaction for a period of 10-100 s. The CO2concentrations ranged from 1.7 to 17 mM for the determination of the kinetic parameters and inhibition constants. For each inhibitor at least six traces of the initial 5-10% of the reaction have been used for determining the initial velocity. The uncatalyzed rates were determined in the same manner and subtracted from the total observed rates. Stock solutions of inhibitor (10 mM) were prepared in distilled-deionized water and dilutions up to 0.01 μM were done thereafter with distilled-deionized water. Inhibitor and enzyme solutions were preincubated together for 15 min at room temperature prior to assay, in order to allow for the formation of the E-I complex. The inhibition constants were obtained by non-linear least-squares methods using the Cheng-Prusoff equation whereas the kinetic parameters for the uninhibited enzymes from Lineweaver-Burk plots, as reported earlier, 27-33and represent the mean from at least three different determinations