Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation

Applied Microbiology and Biotechnology

Volumn 98, Issue 17, 2014, Pages 7535-7548

  Maccani, Andreas ^a,b   Hackl, Matthias ^b   Leitner, Christian ^b   Steinfellner, Willibald ^b   Graf, Alexandra B ^a,c   Tatto, Nadine E ^a   Karbiener, Michael ^d   Scheideler, Marcel ^d   Grillari, Johannes ^b   Mattanovich, Diethard ^a,b   Kunert, Renate ^a,b   Borth, Nicole ^a,b   Grabherr, Reingard ^b   Ernst, Wolfgang ^a,b  

^a AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^c Fachhochschule Campus Wien   (Austria)

^d GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Chemostat; Chinese hamster ovary cells; Microarray; miRNA expression profiling; miRNA target identification; Productivity

Indexed keywords

BIOSYNTHESIS; CELL CULTURE; CELL ENGINEERING; CHEMOSTATS; GENE EXPRESSION; MICROARRAYS; PRODUCTIVITY; RECOMBINANT PROTEINS;

BIOLOGICAL FUNCTIONS; CHINESE HAMSTER OVARY CELLS; HETEROLOGOUS PROTEINS; MICRORNA TARGET PREDICTIONS; MIRNA EXPRESSIONS; MIRNA TARGETS; RECOMBINANT PROTEIN PRODUCTIONS; STEADY-STATE CONDITION;

RNA;

MESSENGER RNA; MICRORNA; RECOMBINANT PROTEIN;

BIOACTIVITY; BIOREACTOR; CULTIVATION; GENE EXPRESSION; MATURATION; PROTEIN; RNA;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL ENGINEERING; CHO CELL LINE; CONTINUOUS CULTURE; CONTROLLED STUDY; DATA BASE; FED BATCH CULTURE; HETEROLOGOUS EXPRESSION; MICROARRAY ANALYSIS; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN SYNTHESIS; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCREENING; STEADY STATE; ANIMAL; CRICETULUS; CULTURE TECHNIQUE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; METABOLISM; PHYSIOLOGY; PROCEDURES;

CRICETULUS GRISEUS;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETULUS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; MICRORNAS;

EID: 84906951509     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5911-4     Document Type: Article

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