Comparative studies on the genotoxicity and cytotoxicity of polymeric gene carriers polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer in Jurkat T-cells

Drug and Chemical Toxicology

Volumn 33, Issue 4, 2010, Pages 357-366

  Choi, Young Joo ^a   Kang, Su Jin ^b   Kim, Yang Jee ^a   Lim, Yong Beom ^c   Chung, Hai Won ^a  

^a Seoul National University   (South Korea)

^b Yeungnam University   (South Korea)

^c Yonsei University   (South Korea)

Author keywords

Apoptosis; CBMN assay; Comet assay; Necrosis; PAMAM; PEI

Indexed keywords

DENDRIMER; DNA; LACTATE DEHYDROGENASE; POLYAMIDOAMINE; POLYETHYLENEIMINE;

ARTICLE; CELL DEATH; COMET ASSAY; CONTROLLED STUDY; CYTOKINESIS; CYTOTOXICITY; DNA DAMAGE; GENOTOXICITY; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; MICRONUCLEUS;

APOPTOSIS; CELL DEATH; CELL SURVIVAL; COMET ASSAY; DENDRIMERS; DRUG CARRIERS; GENE TRANSFER TECHNIQUES; HUMANS; IMINES; JURKAT CELLS; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; MOLECULAR STRUCTURE; NECROSIS; POLYETHYLENES;

EID: 77956167428     PISSN: 01480545     EISSN: 15256014     Source Type: Journal    
DOI: 10.3109/01480540903493507     Document Type: Article

References (35)

1. Arends, M. J., Wyllie, A. H. (1991). Apoptosis: mechanism and role in pathology. Int Rev Exp Pathol 32:223-254.
2. Ariani, F., Mari, F., Pescucci, C., Longo, I., Bruttini, M., Meloni, I., et al. (2004). Real-time quantitative PCR as a routine method for screening large rearrangements in Rett syndrome: report of one case of MECP2 deletion and one case of MECP2 duplication. Hum Mutat 24:172-177.
3. Azzam, T., Domb, A. J. (2006). Current developments in gene transfection agents. Curr Drug Deliv 1:165-193.
4. Bose, S., Yap, L. F., Fung, M., Starzcynski, J., Saleh, A., Morgan, S., et al. (2009). Te ATM tumor suppressor gene is downregu-lated in EBV-associated nasopharyngeal carcinoma. J Pathol 217:345-352.
5. Conrad, D. M., Robichaud, M. R. J., Mader, J. S., Boudreau, R. M., Richardson, A. M., Giacomantonio, C. A., et al. (2008). 2-chlo-ro-2'- deoxyadenosine-induced apoptosis in T-leukemia cells is mediated via a caspase-3-dependent mitochondrial feedback amplifcation loop. Int J Oncol 32:1325-1333.
6. Eastmond, D. A., Tucker, J. D. (1989). Identifcation of aneuploi-dy-inducing agents using cytokinesis-blocked human lymphocytes and an antikinetochore antibody. Environ Mol Mutagen 13:34-43.
7. Fenech, M. (2000). Te in vitro micronucleus techniques. Mutat Res 455:81-95.
8. Godbey, W. T., Wu, K. K., Mikos, A. G. (1999). Tracking the intra-cellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc Natl Acad Sci\USA 96:5177-5181.
9. Green, D. R., Reed, J. C. (1998). Mitochondria and apoptosis. Science 281:1309-1312.
10. Hui, Z., He, Z. G., Zheng, L., Li, G. Y., Shen, S. R., Li, X. L. (2008). Studies on polyamidoamine dendrimers as efcient gene delivery vector. J Biomater Appl 22:527-544.
11. Hunter, A. C. (2006). Molecular hurdles in polyfectin design and mechanistic background to polycation induced cytotoxicity. Adv Drug Del Rev 58:1523-1531.
12. Kanellou, P., Zaravinos, A., Zioga, M., Stratigos, A., Baritaki, S., Soufa, G., et al. (2008). Genomic instability, mutations and expression analysis of the tumor suppressor genes, p14ARF, p15INK4b, p16INK4a, and p53, in actinic keratosis. Cancer Lett 264:145-161.
13. Kang, S. J., Lee, Y. J., Kim, B. M., Kim, Y. J., Woo, H. D., Jeon, H. K., et al. (2008). Efect of Bupleuri radix extracts on the toxicity of 5-fuorouracil in HepG2 hepatoma cells and normal human lymphocytes. Basic Clin Pharmacol Toxicol 103:305-313.
14. Kuo, J. H., Jan, M. S., Chiu, H. W. (2005). Mechanism of cell death induced by cationic dendrimers in RAW 264.7 murine mac-rophage-like cells. J Phanrm Pharmacol 57:489-495.
15. Leist, M., Nicotera, P. (1997). Te shape of cell death. Biochem Biophys Res Commun 236:1-9.
16. Lim, Y. B., Choi, Y. H., Park, J. S. (1999). A self-destroying poly-cationic polymer: biodegradable poly(4-hydroxy-L-proline ester). J Am Chem Soc 121:5633-5639.
17. Lim, Y. B., Kim, C. H., Kim, K., Kim, S. W., Park, J. S. (2000). Development of safe gene delivery system using biodegradable polymer, poly[α-(4-aminobutyl)-l-glycolic acid]. J Am Chem Soc 122:6524-6525.
18. Lim, Y. B., Kim, S. M., Lee, Y., Lee, W. K., Yang, T. G., Park, J. S., et al. (2001). Cationic hyperbranched poly(amino ester): a novel class of DNA condensing molecule with cati-onic surface, biodegradable three-dimensional structure, and tertiary amine groups in the interior. J Am Chem Soc 123:2460-2461.
19. Lim, Y. B., Lee, E. J., Yoon, Y. R., Lee, M. S., Lee, M. (2008). Filamentous artifcial virus from self-assembled discrete nanoribbon. Angew Chem Int Ed 47:4525-4528.
20. Luten, J., Nostrum, C. F., De Smedt, S. C., Hennink, W. E. (2008). Biodegradable polymers as nonviral carriers for plasmid DNA delivery. J Contr Rel 126:97-110.
21. Mastrobattista, E., van der Aa, M. A. E. M., Hennink, W. E., Crommelin, D. J. A. (2006). Artifcial viruses: a nanotech-nological approach to gene delivery. Nat Rev Drug Discov 5:115-121.
22. Moghimi, S. M., Symonds, P., Murray, J. C., Hunter, A. C., Debska, G., Szewczyk, A. (2005). A two-stage poly(ethylenimine)-mediated cytotoxicity: implications for gene transfer/therapy. Mol Ter 11:990-995.
23. Moll, U. M., Zaika, A. (2001). Nuclear and mitochondrial apoptotic pathways of p53. FEBS Lett 493:65-69
24. Napieska, D., Tomassen, L., Rabolli, V., Lison, D., Gonzalez, L., Kirsch-Volders, M., et al. (2009). Size-dependent cytotoxicity of monodisperse silica nanoparticles in human endothelial cells. Small 5:846-853.
25. Okuda, T., Niidome, T., Aoyagi, H. (2004). Cytosolic soluble proteins induce DNA release from DNA-gene carrier compexes. J Contr Rel 98:325-332.
26. Pack, D. W., Hofman, A. S., Pun, S., Stayton, P. S. (2005). Design and development of polymers for gene delivery. Nat Rev Drug Discov 4:581-593.
27. Patil, M. L., Zhang, M., Taratula, O., Garbuzenko, O. B., He, H., Minko, T. (2009). Internally cationic polyamidoam-ine PAMAM-OH dendrimers for siRNA delivery: efect of the degree of quaternization and cancer targeting. Biomacromolecules 10:258-266.
28. Service, R. F. (2008). Nanotechnology: can high-speed tests sort out which nanomaterials are safe? Science 321:1036-1037.
29. Shimizu, S., Eguchi, Y., Kamiike, W., Itoh, Y., Hasegawa, J., Yamabe, K., et al. (1996). Induction of apoptosis as well as necrosis by hypoxia and predominant prevention of apopto-sis by bcl-2 and bcl-XL. Cancer Res 56:2161-2166.
30. Singh, N. P., McCoy, M. T, Tice, R. R., Schneider, E. L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184-191.
31. Suh, J., Wirtz, D., Hanes, J. (2003). Efcient active transport of gene nanocarriers to the cell nucleus. Proc Natl Acad Sci\USA 100:3878-3882.
32. Uhl, M., Laky, B., Lhoste, E., Kassie, F., Kundi M., Knasmüller, S. (2003). Efects of mustard sprouts and allylisothiocyanate on benzo(a)pyrene-induced DNA damage in human-derived cells: a model study with the single cell gel electrophoresis/Hep G2 assay. Teratog Carcinog Mutagen 1:273-282.
33. Wang, J., Mao, H. Q., Leong, K. W. (2001). A novel biodegradable gene carrier based on polyphosphoester. J Am Chem Soc 123:9480-9481.
34. Xiong, M. P., Forrest, M. L., Ton, G., Zhao, A., Davies, N. M., Kwon, G. S. (2007). Poly(aspartate-g-PEI800), a polyethyl-enimine analogue of low toxicity and high transfection ef-ciency for gene delivery. Biomaterials 28:4889-4900.
35. Xu, P., Kirk, E. A. V., Zhan, Y., Murdoch, W. J., Radosz, M., Shen, Y. (2007). Targeted charge-reversal nanoparticles for nuclear drug delivery. Angew Chem Int Ed 46:4999-5002.