Cryptosporidium infections: Molecular advances

Parasitology

Volumn 141, Issue 11, 2014, Pages 1511-1532

  Lendner, Matthias ^a   Daugschies, Arwid ^a  

^a Institute of Parasitology   (Germany)

Author keywords

Actin; Apicomplexa; Attachment; Cryptosporidium; Egress; Host parasite interaction; Invasion; Mucin; Omics

Indexed keywords

ACTIN; CALCIUM; CP12 PROTEIN; CP2 PROTEIN; CP20 PROTEIN; CP21 PROTEIN; CP47 PROTEIN; CPA135 PROTEIN; CPCLEC PROTEIN; CPS 500 PROTEIN; CRYPTOPAIN 1; CRYPTOSTATIN; CSL PROTEIN; EF 1ALPHA PROTEIN; GP40 PROTEIN; GP900 PROTEIN; LECTIN; MUC1 7 PROTEIN; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN; PROTEIN CDC42; PROTEIN P23; PROTEIN P30; TRAP PROTEIN; UNCLASSIFIED DRUG; PROTEOME; TRANSCRIPTOME;

APOPTOSIS; CELL ADHESION; CELL INVASION; CELL MOTILITY; CELL SURVIVAL; CONFERENCE PAPER; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM PARVUM; ENDOPLASMIC RETICULUM; FUNGAL CELL WALL; GAMETOCYTE; GENOMICS; GLYCOCALYX; HOST CELL; HOST PARASITE INTERACTION; HUMAN; LIFE CYCLE; MECHANICAL STRESS; MEROZOITE; NONHUMAN; NUTRITION; OOCYST; PLANT CELL; PRIORITY JOURNAL; PROTEOMICS; SPOROZOITE; TRANSCRIPTOMICS; LIPID RAFT; MEDICAL RESEARCH; REVIEW; SURVIVAL; ANIMAL; CALCIUM SIGNALING; GENETICS; GENOME; LIFE CYCLE STAGE; METABOLISM; PARASITOLOGY; PHYSIOLOGY;

APICOMPLEXA; CRYPTOSPORIDIUM;

ACTINS; ANIMALS; APOPTOSIS; CALCIUM SIGNALING; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM PARVUM; GENOME, PROTOZOAN; HOST-PARASITE INTERACTIONS; HUMANS; LIFE CYCLE STAGES; PROTEOME; TRANSCRIPTOME;

EID: 84906791036     PISSN: 00311820     EISSN: 14698161     Source Type: Journal    
DOI: 10.1017/S0031182014000237     Document Type: Conference Paper

References (165)

1. Abrahamsen, M. S., Templeton, T. J., Enomoto, S., Abrahante, J. E., Zhu, G., Lancto, C. A., Deng, M., Liu, C., Widmer, G., Tzipori, S., Buck, G. A., Xu, P., Bankier, A. T., Dear, P. H., Konfortov, B. A., Spriggs, H. F., Iyer, L., Anantharaman, V., Aravind, L. and Kapur, V. (2004). Complete genome sequence of the apicomplexan, Cryptosporidium parvum. Science (New York, NY) 304, 441-445. doi: 10.1126/science.1094786.
2. Anderson, A. C. (2005). Two crystal structures of dihydrofolate reductasethymidylate synthase from Cryptosporidium hominis reveal protein-ligand interactions including a structural basis for observed antifolate resistance. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 61, 258-262. doi: 10.1107/S1744309105002435.
3. Artz, J. D., Wernimont, A. K., Allali-Hassani, A., Zhao, Y., Amani, M., Lin, Y.-H., Senisterra, G., Wasney, G. A., Fedorov, O., King, O., Roos, A., Lunin, V. V., Qiu, W., Finerty, P., Hutchinson, A., Chau, I., von Delft, F., MacKenzie, F., Lew, J., Kozieradzki, I., Vedadi, M., Schapira, M., Zhang, C., Shokat, K., Heightman, T. and Hui, R. (2011). The Cryptosporidium parvum kinome. BMC Genomics 12, 478. doi: 10.1186/1471-2164-12-478.
4. Atuma, C., Strugala, V., Allen, A. and Holm, L. (2001). The adherent gastrointestinal mucus gel layer: thickness and physical state in vivo. American Journal of Physiology. Gastrointestinal and Liver Physiology 280, G922-G929.
5. Barnes, D. A., Bonnin, A., Huang, J. X., Gousset, L., Wu, J., Gut, J., Doyle, P., Dubremetz, J. F., Ward, H. and Petersen, C. (1998). A novel multi-domain mucin-like glycoprotein of Cryptosporidium parvum mediates invasion. Molecular and Biochemical Parasitology 96, 93-110. doi: 10.1016/ S0166-6851(98)00119-4.
6. Barta, J. R. and Thompson, R. C. A. (2006). What is Cryptosporidium? Reappraising its biology and phylogenetic affinities. Trends in Parasitology 22, 463-468. doi: 10.1016/j.pt.2006.08.001.
7. Baum, J., Papenfuss, A. T., Baum, B., Speed, T. P. and Cowman, A. F. (2006). Regulation of apicomplexan actin-based motility. Nature Reviews. Microbiology 4, 621-628. doi: 10.1038/nrmicro1465.
8. Baum, J., Gilberger, T.-W., Frischknecht, F. and Meissner, M. (2008). Host-cell invasion by malaria parasites: insights from Plasmodium and Toxoplasma. Trends in Parasitology 24, 557-563. doi: 10.1016/j.pt.2008.08.006.
9. Belli, S. I., Witcombe, D., Wallach, M. G. and Smith, N. C. (2002). Functional genomics of gam56: characterisation of the role of a 56 kilodalton sexual stage antigen in oocyst wall formation in Eimeria maxima. International Journal for Parasitology 32, 1727-1737.
10. Beyer, T. (2002). Parasitophorous vacuole: morphofunctional diversity in different Coccidian genera (a short insight into the problem). Cell Biology International 26, 861-871. doi: 10.1006/cbir.2002.0943.
11. Bhalchandra, S., Ludington, J., Coppens, I. and Ward, H. D. (2013). Identification and characterization of Cryptosporidium parvum Clec, a novel C-type lectin domain-containing mucin-like glycoprotein. Infection and Immunity 81, 3356-3365. doi: 10.1128/IAI.00436-13.
12. Bhat, N., Joe, A., PereiraPerrin, M. and Ward, H. D. (2007). Cryptosporidium p30, a galactose/N-acetylgalactosamine-specific lectin, mediates infection in vitro. Journal of Biological Chemistry 282, 34877-34887. doi: 10.1074/jbc.M706950200.
13. Billker, O., Lourido, S. and Sibley, L.D. (2009). Calcium-dependent signaling and kinases in apicomplexan parasites. Cell Host & Microbe 5, 612-622. doi: 10.1016/j.chom.2009.05.017.
14. Bonafonte, M. T., Smith, L. M. and Mead, J. R. (2000). A 23-kDa recombinant antigen of Cryptosporidium parvum induces a cellular immune response on in vitro stimulated spleen and mesenteric lymph node cells from infected mice. Experimental Parasitology 96, 32-41. doi: 10.1006/expr.2000.4545.
15. Bonhomme, A., Bouchot, A., Pezzella, N., Gomez, J., Le Moal, H. and Pinon, J. M. (1999). Signaling during the invasion of host cells by Toxoplasma gondii. FEMS Microbiology Reviews 23, 551-561.
16. Bonnin, A., Lapillonne, A., Petrella, T., Lopez, J., Chaponnier, C., Gabbiani, G., Robine, S. and Dubremetz, J. F. (1999). Immunodetection of the microvillous cytoskeleton molecules villin and ezrin in the parasitophorous vacuole wall of Cryptosporidium parvum (Protozoa: Apicomplexa). European Journal of Cell Biology 78, 794-801.
17. Bouzid, M., Tyler, K. M., Christen, R., Chalmers, R. M., Elwin, K. and Hunter, P. R. (2010). Multi-locus analysis of human infective Cryptosporidium species and subtypes using ten novel genetic loci. BMC Microbiology 10, 213. doi: 10.1186/1471-2180-10-213.
18. Bouzid, M., Hunter, P. R., Chalmers, R.M. and Tyler, K. M. (2013a). Cryptosporidium pathogenicity and virulence. Clinical Microbiology Reviews 26, 115-134. doi: 10.1128/CMR.00076-12.
19. Bouzid, M., Hunter, P. R., McDonald, V., Elwin, K., Chalmers, R.M. and Tyler, K. M. (2013b). A new heterogeneous family of telomerically encoded Cryptosporidium proteins. Evolutionary Applications 6, 207-217. doi: 10.1111/j.1752-4571.2012.00277.x.
20. Bushkin, G. G., Motari, E., Carpentieri, A., Dubey, J. P., Costello, C. E., Robbins, P.W. and Samuelson, J. (2013). Evidence for a structural role for acid-fast lipids in oocyst walls of Cryptosporidium, Toxoplasma, and Eimeria. mBio 4, e00387-e00313. doi: 10.1128/mBio.00387-13.
21. Cai, X., Herschap, D. and Zhu, G. (2005). Functional characterization of an evolutionarily distinct phosphopantetheinyl transferase in the apicomplexan Cryptosporidium parvum. Eukaryotic Cell 4, 1211-1220. doi: 10.1128/EC.4.7.1211- 1220.2005.
22. Camero, L., Shulaw, W. P. and Xiao, L. (2003). Characterization of a Cryptosporidium parvum gene encoding a protein with homology to long chain fatty acid synthetase. Journal of Eukaryotic Microbiology 50 (Suppl.), 534-538.
23. Carreno, R. A., Martin, D. S. and Barta, J. R. (1999). Cryptosporidium is more closely related to the gregarines than to coccidia as shown by phylogenetic analysis of apicomplexan parasites inferred using small-subunit ribosomal RNA gene sequences. Parasitology Research 85, 899-904. doi: 10.1007/s004360050655.
24. Carryn, S., Schaefer, D. A., Imboden, M., Homan, E. J., Bremel, R. D. and Riggs, M.W. (2012). Phospholipases and cationic peptides inhibit Cryptosporidium parvum sporozoite infectivity by parasiticidal and non-parasiticidal mechanisms. Journal of Parasitology 98, 199-204. doi: 10.1645/GE-2822.1.
25. Cevallos, A. M., Zhang, X., Waldor, M. K., Jaison, S., Zhou, X., Tzipori, S., Neutra, M. R. and Ward, H. D. (2000a). Molecular cloning and expression of a gene encoding Cryptosporidium parvum glycoproteins gp40 and gp15. Infection and Immunity 68, 4108-4116.
26. Cevallos, A. M., Bhat, N., Verdon, R., Hamer, D. H., Stein, B., Tzipori, S., Pereira, M. E. A., Keusch, G. T. and Ward, H. D. (2000b). Mediation of Cryptosporidium parvum infection in vitro by mucin-like glycoproteins defined by a neutralizing monoclonal antibody. Infection and Immunity 68, 5167-5175. doi: 10.1128/IAI.68.9.5167-5175.2000.
27. Chandramohanadas, R., Davis, P. H., Beiting, D. P., Harbut, M. B., Darling, C., Velmourougane, G., Lee, M. Y., Greer, P. A., Roos, D. S. and Greenbaum, D. C. (2009). Apicomplexan parasites co-opt host calpains to facilitate their escape from infected cells. Science 324, 794-797. doi: 10.1126/science.1171085.
28. Chatterjee, A., Banerjee, S., Steffen, M., O'Connor, R. M., Ward, H. D., Robbins, P.W. and Samuelson, J. (2010). Evidence for mucin-like glycoproteins that tether sporozoites of Cryptosporidium parvum to the inner surface of the oocyst wall. Eukaryotic Cell 9, 84-96. doi: 10.1128/ EC.00288-09.
29. Chen, X. M. and LaRusso, N. F. (2000). Mechanisms of attachment and internalization of Cryptosporidium parvum to biliary and intestinal epithelial cells. Gastroenterology 118, 368-379.
30. Chen, X. M., Levine, S. A., Tietz, P., Krueger, E., McNiven, M. A., Jefferson, D. M., Mahle, M. and LaRusso, N. F. (1998). Cryptosporidium parvum is cytopathic for cultured human biliary epithelia via an apoptotic mechanism. Hepatology 28, 906-913. doi: 10.1002/hep.510280402.
31. Chen, X. M., Gores, G. J., Paya, C. V and LaRusso, N. F. (1999). Cryptosporidium parvum induces apoptosis in biliary epithelia by a Fas/Fas ligand-dependent mechanism. American Journal of Physiology 277, G599-G608.
32. Chen, X.-M., Huang, B. Q., Splinter, P. L., Cao, H., Zhu, G., McNiven, M. A. and LaRusso, N. F. (2003). Cryptosporidium parvum invasion of biliary epithelia requires host cell tyrosine phosphorylation of cortactin via c-Src. Gastroenterology 125, 216-228. doi: 10.1016/S0016-5085(03)00662-0.
33. Chen, X.-M., Huang, B. Q., Splinter, P. L., Orth, J. D., Billadeau, D. D., McNiven, M. A. and LaRusso,N. F. (2004a). Cdc42 and the actin-related protein/neural Wiskott-Aldrich syndrome protein network mediate cellular invasion by Cryptosporidium parvum. Infection and Immunity 72, 3011-3021. doi: 10.1128/IAI.72.5.3011-3021.2004.
34. Chen, X.-M., Splinter, P. L., Tietz, P. S., Huang, B. Q., Billadeau, D. D. and LaRusso, N. F. (2004b). Phosphatidylinositol 3-kinase and frabin mediate Cryptosporidium parvum cellular invasion via activation of Cdc42. Journal of Biological Chemistry 279, 31671-31678. doi: 10.1074/jbc.M401592200.
35. Chen, X., O'Hara, S. P., Huang, B. Q., Nelson, J. B., Lin, J. J., Zhu, G., Ward, H. D. and LaRusso, N. F. (2004c). Apical organelle discharge by Cryptosporidium parvum is temperature, cytoskeleton, and intracellular calcium dependent and required for host cell invasion. Infection and Immunity 72, 6806-6816. doi: 10.1128/IAI.72.12.6806-6816.2004.
36. Chen, X.-M., O'Hara, S. P., Huang, B. Q., Splinter, P. L., Nelson, J. B. and LaRusso, N. F. (2005). Localized glucose and water influx facilitates Cryptosporidium parvum cellular invasion by means of modulation of hostcell membrane protrusion. Proceedings of the National Academy of Sciences USA 102, 6338-6343. doi: 10.1073/pnas.0408563102.
37. Cook, W. J., Senkovich, O., Aleem, K. and Chattopadhyay, D. (2012). Crystal structure of Cryptosporidium parvum pyruvate kinase. PloS One 7, e46875. doi: 10.1371/journal.pone.0046875.
38. Ctrnáctá, V., Stejskal, F., Keithly, J. S. and Hrdý, I. (2007). Characterization of S-adenosylhomocysteine hydrolase from Cryptosporidium parvum. FEMS Microbiology Letters 273, 87-95. doi: 10.1111/j.1574-6968.2007.00795.x.
39. Deng, M., Templeton, T. J., London, N. R., Bauer, C., Schroeder, A. A. and Abrahamsen, M. S. (2002). Cryptosporidium parvum genes containing thrombospondin type 1 domains. Infection and Immunity 70, 6987-6995.
40. Doan, L. T., Martucci, W. E., Vargo, M. A., Atreya, C. E. and Anderson, K. S. (2007). Nonconserved residues Ala287 and Ser290 of the Cryptosporidium hominis thymidylate synthase domain facilitate its rapid rate of catalysis. Biochemistry 46, 8379-8391. doi: 10.1021/ bi700531r.
41. Ehrenman, K., Wanyiri, J.W., Bhat, N., Ward, H. D. and Coppens, I. (2013). Cryptosporidium parvum scavenges LDL-derived cholesterol and micellar cholesterol internalized into enterocytes. Cellular Microbiology 15, 1182-1197. doi: 10.1111/cmi.12107.
42. Enriquez, F. J. and Riggs, M.W. (1998). Role of immunoglobulin A monoclonal antibodies against P23 in controlling murine Cryptosporidium parvum infection. Infection and Immunity 66, 4469-4473.
43. Fayer, R. and Leek, R. G. (1984). The effects of reducing conditions, medium, pH, temperature, and time on in vitro excystation of Cryptosporidium. Journal of Protozoology 31, 567-569.
44. Fayer, R. and Xiao, L. (ed.) (2008). Cryptosporidium and Cryptosporidiosis, 2nd Edn. (ed. Fayer, R. and Xiao, L.). CRC Press, New York, NY, USA.
45. Forney, J. R., Yang, S., Du, C. and Healey, M. C. (1996a). Efficacy of serine protease inhibitors against Cryptosporidium parvum infection in a bovine fallopian tube epithelial cell culture system. Journal of Parasitology 82, 638-640.
46. Forney, J. R., Yang, S. and Healey, M. C. (1996b). Protease activity associated with excystation of Cryptosporidium parvum oocysts. Journal of Parasitology 82, 889-892.
47. Forney, J. R., DeWald, D. B., Yang, S., Speer, C. A. and Healey, M. C. (1999). A role for host phosphoinositide 3-kinase and cytoskeletal remodeling during Cryptosporidium parvum infection. Infection and Immunity 67, 844-852.
48. Foster, D. M., Stauffer, S. H., Stone, M. R. and Gookin, J. L. (2012). Proteasome inhibition of pathologic shedding of enterocytes to defend barrier function requires X-linked inhibitor of apoptosis protein and nuclear factor ?B. Gastroenterology 143, 133-144.e4. doi: 10.1053/j.gastro.2012.03.030.
49. Franco, S. J. and Huttenlocher, A. (2005). Regulating cell migration: calpains make the cut. Journal of Cell Science 118, 3829-3838. doi: 10.1242/jcs.02562.
50. Fritzler, J. M. and Zhu, G. (2007). Functional characterization of the acyl-[acyl carrier protein] ligase in the Cryptosporidium parvum giant polyketide synthase. International Journal for Parasitology 37, 307-316. doi: 10.1016/j.ijpara.2006.10.014.
51. Fritzler, J. M., Millership, J. J. and Zhu, G. (2007). Cryptosporidium parvum long-chain fatty acid elongase. Eukaryotic Cell 6, 2018-2028. doi: 10.1128/EC.00210-07.
52. Galazka, J., Striepen, B. and Ullman, B. (2006). Adenosine kinase from Cryptosporidium parvum. Molecular and Biochemical Parasitology 149, 223-230. doi: 10.1016/j.molbiopara.2006.06.001.
53. Gardner, M. J., Bishop, R., Shah, T., de Villiers, E. P., Carlton, J. M., Hall, N., Ren, Q., Paulsen, I. T., Pain, A., Berriman, M., Wilson, R. J. M., Sato, S., Ralph, S. A., Mann, D. J., Xiong, Z., Shallom, S. J., Weidman, J., Jiang, L., Lynn, J., Weaver, B., Shoaibi, A., Domingo, A. R., Wasawo, D., Crabtree, J., Wortman, J. R., Haas, B., Angiuoli, S. V., Creasy, T. H., Lu, C., Suh, B., Silva, J. C., Utterback, T. R., Feldblyum, T. V., Pertea, M., Allen, J., Nierman, W. C., Taracha, E. L. N., Salzberg, S. L., White, O. R., Fitzhugh, H. A., Morzaria, S., Venter, J. C., Fraser, C. M. and Nene, V. (2005). Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes. Science (New York, NY) 309, 134-137. doi: 10.1126/science.1110439.
54. Geriletu, Xu, R., Jia, H., Terkawi, M. A., Xuan, X. and Zhang, H. (2011). Immunogenicity of orally administrated recombinant Lactobacillus casei Zhang expressing Cryptosporidium parvum surface adhesion protein P23 in mice. Current Microbiology 62, 1573-1580. doi: 10.1007/s00284-011-9894-4.
55. Gong, A.-Y., Zhou, R., Hu, G., Liu, J., Sosnowska, D., Drescher, K. M., Dong, H. and Chen, X.-M. (2010). Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513. Journal of Infectious Diseases 201, 160-169. doi: 10.1086/648589.
56. Grüner, A. C., Snounou, G., Fuller, K., Jarra, W., Rénia, L. and Preiser, P. R. (2004). The Py235 proteins: glimpses into the versatility of a malaria multigene family. Microbes and Infection 6, 864-873. doi: 10.1016/ j.micinf.2004.04.004.
57. Hashim, A., Mulcahy, G., Bourke, B. and Clyne, M. (2006). Interaction of Cryptosporidium hominis and Cryptosporidium parvum with primary human and bovine intestinal cells. Infection and Immunity 74, 99-107. doi:10.1128/IAI.74.1.99- 107.2006.
58. Heiges, M., Wang, H., Robinson, E., Aurrecoechea, C., Gao, X., Kaluskar, N., Rhodes, P., Wang, S., He, C.-Z., Su, Y., Miller, J., Kraemer, E. and Kissinger, J. C. (2006). CryptoDB: a Cryptosporidium bioinformatics resource update. Nucleic Acids Research 34, D419-D422. doi: 10.1093/nar/gkj078.
59. Huang, J., Mullapudi, N., Lancto, C. A., Scott, M., Abrahamsen, M. S. and Kissinger, J. C. (2004a). Phylogenomic evidence supports past endosymbiosis, intracellular and horizontal gene transfer in Cryptosporidium parvum. Genome Biology 5, R88. doi: 10.1186/gb-2004-5-11-r88.
60. Huang, B. Q., Chen, X.-M. and LaRusso, N. F. (2004b). Cryptosporidium parvum attachment to and internalization by human biliary epithelia in vitro: a morphologic study. Journal of Parasitology 90, 212-221.
61. Jakobi, V. and Petry, F. (2006). Differential expression of Cryptosporidium parvum genes encoding sporozoite surface antigens in infected HCT-8 host cells. Microbes and Infection 8, 2186-2194. doi: 10.1016/j.micinf. 2006.04.012.
62. Jenkins, M. C., Fayer, R., Tilley, M. and Upton, S. J. (1993). Cloning and expression of a cDNA encoding epitopes shared by 15-and 60-kilodalton proteins of Cryptosporidium parvum sporozoites. Infection and Immunity 61, 2377-2382.
63. Jenkins, M. C., O'Brien, C., Miska, K., Schwarz, R. S., Karns, J., Santin, M. and Fayer, R. (2011). Gene expression during excystation of Cryptosporidium parvum oocysts. Parasitology Research 109, 509-513. doi: 10.1007/s00436-011-2308-5.
64. Joe, A., Hamer, D. H., Kelley, M. A., Pereira, M. E., Keusch, G. T., Tzipori, S. and Ward, H. D. (1994). Role of a Gal/GalNAc-specific sporozoite surface lectin in Cryptosporidium parvum-host cell interaction. Journal of Eukaryotic Microbiology 41, 44S.
65. Joe, A., Verdon, R., Tzipori, S., Keusch, G. T. and Ward, H. D. (1998). Attachment of Cryptosporidium parvum sporozoites to human intestinal epithelial cells. Infection and Immunity 66, 3429-3432.
66. Kafsack, B. F. C., Pena, J. D. O., Coppens, I., Ravindran, S., Boothroyd, J. C. and Carruthers, V. B. (2009). Rapid membrane disruption by a perforin-like protein facilitates parasite exit from host cells. Science 323, 530-533. doi: 10.1126/science.1165740.
67. Kang, J.-M., Ju, H.-L., Yu, J.-R., Sohn, W.-M. and Na, B.-K. (2012). Cryptostatin, a chagasin-family cysteine protease inhibitor of Cryptosporidium parvum. Parasitology 139, 1029-1037. doi: 10.1017/S0031182012000297.
68. Keithly, J. S., Zhu, G., Upton, S. J., Woods, K. M., Martinez, M. P. and Yarlett, N. (1997). Polyamine biosynthesis in Cryptosporidium parvum and its implications for chemotherapy. Molecular and Biochemical Parasitology 88, 35-42.
69. King, B. J., Keegan, A. R., Phillips, R., Fanok, S. and Monis, P. T. (2012). Dissection of the hierarchy and synergism of the bile derived signal on Cryptosporidium parvum excystation and infectivity. Parasitology 139, 1533-1546. doi: 10.1017/S0031182012000984.
70. Lamkanfi, M. and Dixit, V. M. (2010). Manipulation of host cell death pathways during microbial infections. Cell Host & Microbe 8, 44-54. doi:10.1016/j.chom.2010.06.007.
71. Langer, R. C. and Riggs, M.W. (1999). Cryptosporidium parvum apical complex glycoprotein CSL contains a sporozoite ligand for intestinal epithelial cells. Infection and Immunity 67, 5282-5291.
72. Langer, R. C., Schaefer, D. A. and Riggs, M.W. (2001). Characterization of an intestinal epithelial cell receptor recognized by the Cryptosporidium parvum sporozoite ligand CSL. Infection and Immunity 69, 1661-1670. doi: 10.1128/IAI.69.3.1661-1670.2001.
73. Lebart, M.-C. and Benyamin, Y. (2006). Calpain involvement in the remodeling of cytoskeletal anchorage complexes. FEBS Journal 273, 3415-3426. doi: 10.1111/j.1742-4658.2006.05350.x.
74. Li, W., Zhang, N., Liang, X., Li, J., Gong, P., Yu, X., Ma, G., Ryan, U. M. and Zhang, X. (2009). Transient transfection of Cryptosporidium parvum using green fluorescent protein (GFP) as a marker. Molecular and Biochemical Parasitology 168, 143-148. doi: 10.1016/j. molbiopara.2009.07.003.
75. Lidell, M. E., Moncada, D. M., Chadee, K. and Hansson, G. C. (2006). Entamoeba histolytica cysteine proteases cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel. Proceedings of the National Academy of Sciences USA 103, 9298-9303. doi: 10.1073/pnas.0600623103.
76. Lourido, S., Shuman, J., Zhang, C., Shokat, K. M., Hui, R. and Sibley, L. D. (2010). Calcium-dependent protein kinase 1 is an essential regulator of exocytosis in Toxoplasma. Nature 465, 359-362. doi: 10.1038/nature09022.
77. Lüder, C. G., Gross, U. and Lopes, M. F. (2001). Intracellular protozoan parasites and apoptosis: diverse strategies to modulate parasite-host interactions. Trends in Parasitology 17, 480-486.
78. Martin, K. H., Slack, J. K., Boerner, S. A., Martin, C. C. and Parsons, J. T. (2002). Integrin connections map: to infinity and beyond. Science (New York, NY) 296, 1652-1653. doi: 10.1126/science.296.5573.1652.
79. Martucci, W. E., Vargo, M. A. and Anderson, K. S. (2008). Explaining an unusually fast parasitic enzyme: folate tail-binding residues dictate substrate positioning and catalysis in Cryptosporidium hominis thymidylate synthase. Biochemistry 47, 8902-8911. doi: 10.1021/bi800466z.
80. Matsubayashi, M., Teramoto-Kimata, I., Uni, S., Lillehoj, H. S., Matsuda, H., Furuya, M., Tani, H. and Sasai, K. (2013). Elongation factor-1a is a novel protein associated with host cell invasion and a potential protective antigen of Cryptosporidium parvum. Journal of Biological Chemistry 288, 34111-34120. doi: 10.1074/jbc.M113.515544.
81. Mauzy, M. J., Enomoto, S., Lancto, C. A., Abrahamsen, M. S. and Rutherford, M. S. (2012). The Cryptosporidium parvum transcriptome during in vitro development. PloS One 7, e31715. doi: 10.1371/journal.pone.0031715.
82. McCole, D. F., Eckmann, L., Laurent, F. and Kagnoff, M.F. (2000). Intestinal epithelial cell apoptosis following Cryptosporidium parvum infection. Infection and Immunity 68, 1710-1713. doi: 10.1128/IAI.68.3.1710-1713.2000.
83. Moncada, D., Keller, K. and Chadee, K. (2003). Entamoeba histolytica cysteine proteinases disrupt the polymeric structure of colonic mucin and alter its protective function. Infection and Immunity 71, 838-844.
84. Morada, M., Pendyala, L., Wu, G., Merali, S. and Yarlett, N. (2013). Cryptosporidium parvum induces an endoplasmic stress response in the intestinal adenocarcinoma HCT-8 cell line. Journal of Biological Chemistry 288, 30356-30364. doi: 10.1074/jbc.M113.459735.
85. Morahan, B. J., Wang, L. and Coppel, R. L. (2009). No TRAP, no invasion. Trends in Parasitology 25, 77-84. doi: 10.1016/j.pt.2008.11.004.
86. Moreno, S.N. J. and Docampo, R. (2003). Calcium regulation in protozoan parasites. Current Opinion in Microbiology 6, 359-364. doi: 10.1016/S1369- 5274(03)00091-2.
87. Murphy, R. C., Ojo, K. K., Larson, E. T., Castellanos-Gonzalez, A., Perera, B. G. K., Keyloun, K. R., Kim, J. E., Bhandari, J. G., Muller, N. R., Verlinde, C. L. M. J., White, A. C., Merritt, E. A., Van Voorhis, W. C. and Maly, D. J. (2010). Discovery of potent and selective inhibitors of calcium-dependent protein kinase 1 (CDPK1) from C. parvum and T. gondii. ACS Medicinal Chemistry Letters 1, 331-335. doi:10.1021/ml100096t.
88. Na, B.-K., Kang, J.-M., Cheun, H.-I., Cho, S.-H., Moon, S.-U., Kim, T.-S. and Sohn, W.-M. (2009). Cryptopain-1, a cysteine protease of Cryptosporidium parvum, does not require the pro-domain for folding. Parasitology 136, 149-157. doi: 10.1017/S0031182008005350.
89. Naitza, S., Spano, F., Robson, K. J. and Crisanti, A. (1998). The thrombospondin-related protein family of apicomplexan parasites: the gears of the cell invasion machinery. Parasitology Today 14, 479-484.
90. Ndao, M., Nath-Chowdhury, M., Sajid, M., Marcus, V., Mashiyama, S. T., Sakanari, J., Chow, E., Mackey, Z., Land, K. M., Jacobson, M. P., Kalyanaraman, C., McKerrow, J. H., Arrowood, M. J. and Caffrey, C. R. (2013). A cysteine protease inhibitor rescues mice from a lethal Cryptosporidium parvum infection. Antimicrobial Agents and Chemotherapy 57, 6063-6073. doi: 10.1128/AAC.00734-13.
91. Nelson, J. B., O'Hara, S. P., Small, A. J., Tietz, P. S., Choudhury, A. K., Pagano, R. E., Chen, X. and LaRusso, N. F. (2006). Cryptosporidium parvum infects human cholangiocytes via sphingolipid-enriched membrane microdomains. Cellular Microbiology 8, 1932-1945. doi: 10.1111/j.1462-5822.2006.00759.x.
92. Nesterenko, M. V., Woods, K. and Upton, S. J. (1999). Receptor/ligand interactions between Cryptosporidium parvum and the surface of the host cell. Biochimica et Biophysica Acta 1454, 165-173.
93. O'Connor, R. M., Kim, K., Khan, F. and Ward, H. D. (2003). Expression of Cpgp40/15 in Toxoplasma gondii: a surrogate system for the study of Cryptosporidium glycoprotein antigens. Infection and Immunity 71, 6027-6034.
94. O'Connor, R. M., Wanyiri, J.W., Wojczyk, B. S., Kim, K. and Ward, H. (2007a). Stable expression of Cryptosporidium parvum glycoprotein gp40/15 in Toxoplasma gondii. Molecular and Biochemical Parasitology 152, 149-158. doi: 10.1016/j.molbiopara.2007.01.003.
95. O'Connor, R. M., Wanyiri, J.W., Cevallos, A. M., Priest, J.W. and Ward, H. D. (2007b). Cryptosporidium parvum glycoprotein gp40 localizes to the sporozoite surface by association with gp15. Molecular and Biochemical Parasitology 156, 80-83. doi: 10.1016/j.molbiopara.2007.07.010.
96. O'Connor, R. M., Burns, P. B., Ha-Ngoc, T., Scarpato, K., Khan, W., Kang, G. and Ward, H. (2009). Polymorphic mucin antigens CpMuc4 and CpMuc5 are integral to Cryptosporidium parvum infection in vitro. Eukaryotic Cell 8, 461-469. doi: 10.1128/EC.00305-08.
97. O'Hara, S. P., Yu, J.-R. and Lin, J. J.-C. (2004). A novel Cryptosporidium parvum antigen, CP2, preferentially associates with membranous structures. Parasitology Research 92, 317-327. doi: 10.1007/s00436-003-1057-5.
98. O'Hara, S. P., Gajdos, G. B., Trussoni, C. E., Splinter, P. L. and LaRusso, N. F. (2010). Cholangiocyte myosin IIB is required for localized aggregation of sodium glucose cotransporter 1 to sites of Cryptosporidium parvum cellular invasion and facilitates parasite internalization. Infection and Immunity 78, 2927-2936. doi: 10.1128/IAI.00077-10.
99. Oberstaller, J., Joseph, S. J. and Kissinger, J. C. (2013). Genome-wide upstream motif analysis of Cryptosporidium parvum genes clustered by expression profile. BMC Genomics 14, 516. doi: 10.1186/1471-2164-14-516.
100. Ojcius, D. M., Perfettini, J. L., Bonnin, A. and Laurent, F. (1999). Caspase-dependent apoptosis during infection with Cryptosporidium parvum. Microbes and Infection 1, 1163-1168.
101. Okhuysen, P. C., Chappell, C. L., Kettner, C. and Sterling, C. R. (1996). Cryptosporidium parvum metalloaminopeptidase inhibitors prevent in vitro excystation. Antimicrobial Agents and Chemotherapy 40, 2781-2784.
102. Okhuysen, P. C., Rogers, G. A., Crisanti, A., Spano, F., Huang, D. B., Chappell, C. L. and Tzipori, S. (2004). Antibody response of healthy adults to recombinant thrombospondin-related adhesive protein of Cryptosporidium 1 after experimental exposure to Cryptosporidium oocysts. Clinical and Diagnostic Laboratory Immunology 11, 235-238. doi: 10.1128/CDLI.11.2.235-238.2004.
103. Padda, R. S., Tsai, A., Chappell, C. L. and Okhuysen, P. C. (2002). Molecular cloning and analysis of the Cryptosporidium parvum aminopeptidase N gene. International Journal for Parasitology 32, 187-197.
104. Perez-Cordon, G., Nie, W., Schmidt, D., Tzipori, S. and Feng, H. (2011). Involvement of host calpain in the invasion of Cryptosporidium parvum. Microbes and Infection/Institut Pasteur 13, 103-107. doi: 10.1016/j.micinf.2010.10.007.
105. Perkins, M. E. (1999). CpABC, a Cryptosporidium parvum ATP-binding cassette protein at the host-parasite boundary in intracellular stages. Proceedings of the National Academy of Sciences USA 96, 5734-5739. doi:10.1073/pnas.96.10.5734.
106. Perryman, L. E., Riggs, M.W., Mason, P.H. and Fayer, R. (1990). Kinetics of Cryptosporidium parvum sporozoite neutralization by monoclonal antibodies, immune bovine serum, and immune bovine colostrum. Infection and Immunity 58, 257-259.
107. Perryman, L. E., Kapil, S. J., Jones, M. L. and Hunt, E. L. (1999). Protection of calves against cryptosporidiosis with immune bovine colostrum induced by a Cryptosporidium parvum recombinant protein. Vaccine 17, 2142-2149.
108. Petersen, C., Gut, J., Doyle, P. S., Crabb, J. H., Nelson, R. G. and Leech, J. H. (1992). Characterization of a >900000-M(r) Cryptosporidium parvum sporozoite glycoprotein recognized by protective hyperimmune bovine colostral immunoglobulin. Infection and Immunity 60, 5132-5138.
109. Pollok, R. C. G., McDonald, V., Kelly, P. and Farthing, M. J. G. (2003). The role of Cryptosporidium parvum-derived phospholipase in intestinal epithelial cell invasion. Parasitology Research 90, 181-186. doi: 10.1007/ s00436-003-0831-8.
110. Puiu, D., Enomoto, S., Buck, G. A., Abrahamsen, M. S. and Kissinger, J. C. (2004). CryptoDB: the Cryptosporidium genome resource. Nucleic Acids Research 32, D329-D331. doi: 10.1093/nar/gkh050.
111. Putignani, L., Possenti, A., Cherchi, S., Pozio, E., Crisanti, A. and Spano, F. (2008). The thrombospondin-related protein CpMIC1 (CpTSP8) belongs to the repertoire of micronemal proteins of Cryptosporidium parvum. Molecular and Biochemical Parasitology 157, 98-101. doi: 10.1016/j.molbiopara.2007.09.004.
112. Reduker, D.W. and Speer, C. A. (1985). Factors influencing excystation in Cryptosporidium oocysts from cattle. Journal of Parasitology 71, 112-115.
113. Reduker, D.W., Speer, C. A. and Blixt, J. A. (1985). Ultrastructure of Cryptosporidium parvum oocysts and excysting sporozoites as revealed by high resolution scanning electron microscopy. Journal of Protozoology 32, 708-711.
114. Rider, S. D. and Zhu, G. (2010). Cryptosporidium: genomic and biochemical features. Experimental Parasitology 124, 2-9. doi: 10.1016/ j.exppara.2008.12.014.
115. Riggs, M.W., McGuire, T. C., Mason, P.H. and Perryman, L. E. (1989). Neutralization-sensitive epitopes are exposed on the surface of infectious Cryptosporidium parvum sporozoites. Journal of Immunology 143, 1340-1345.
116. Riggs, M.W., Cama, V. A., Leary, H. L. and Sterling, C. R. (1994). Bovine antibody against Cryptosporidium parvum elicits a circumsporozoite precipitate-like reaction and has immunotherapeutic effect against persistent cryptosporidiosis in SCID mice. Infection and Immunity 62, 1927-1939.
117. Riggs, M.W., Schaefer, D. A., Kapil, S. J., Barley-Maloney, L., Perryman, L. E. and McNeil, M. R. (2001). Targeted disruption of CSL ligand-host cell receptor interaction in treatment of Cryptosporidium parvum infection. Journal of Eukaryotic Microbiology (Suppl.), 44S-46S.
118. Riggs, M.W., Schaefer, D. A., Kapil, S. J., Barley-Maloney, L. and Perryman, L. E. (2002). Efficacy of monoclonal antibodies against defined antigens for passive immunotherapy of chronic gastrointestinal cryptosporidiosis. Antimicrobial Agents and Chemotherapy 46, 275-282.
119. Robertson, L. J., Campbell, A. T. and Smith, H. V. (1993). In vitro excystation of Cryptosporidium parvum. Parasitology 106, 13-19.
120. Roiko, M. S. and Carruthers, V. B. (2009). New roles for perforins and proteases in apicomplexan egress. Cellular Microbiology 11, 1444-1452. doi: 10.1111/j.1462-5822.2009.01357.x.
121. Sanderson, S. J., Xia, D., Prieto, H., Yates, J., Heiges, M., Kissinger, J. C., Bromley, E., Lal, K., Sinden, R. E., Tomley, F. and Wastling, J. M. (2008). Determining the protein repertoire of Cryptosporidium parvum sporozoites. Proteomics 8, 1398-1414. doi: 10.1002/pmic.200700804.
122. Sasahara, T., Maruyama, H., Aoki, M., Kikuno, R., Sekiguchi, T., Takahashi, A., Satoh, Y., Kitasato, H., Takayama, Y. and Inoue, M. (2003). Apoptosis of intestinal crypt epithelium after Cryptosporidium parvum infection. Journal of Infection and Chemotherapy 9, 278-281. doi: 10.1007/s10156-003-0259- 1.
123. Schaefer, D. A., Auerbach-Dixon, B. A. and Riggs, M.W. (2000). Characterization and formulation of multiple epitope-specific neutralizing monoclonal antibodies for passive immunization against cryptosporidiosis. Infection and Immunity 68, 2608-2616.
124. Sestak, K., Ward, L. A., Sheoran, A., Feng, X., Akiyoshi, D. E., Ward, H. D. and Tzipori, S. (2002). Variability among Cryptosporidium parvum genotype 1 and 2 immunodominant surface glycoproteins. Parasite Immunology 24, 213-219.
125. Sharman, P. A., Smith, N. C., Wallach, M. G. and Katrib, M. (2010). Chasing the golden egg: vaccination against poultry coccidiosis. Parasite Immunology 32, 590-598. doi: 10.1111/j.1365-3024.2010.01209.x.
126. Sibley, L. D. (2010). How apicomplexan parasites move in and out of cells. Current Opinion in Biotechnology 21, 592-598. doi: 10.1016/j.copbio.2010. 05.009.
127. Siddiki, A. Z. (2013). Sporozoite proteome analysis of Cryptosporidium parvum by one-dimensional SDS-PAGE and liquid chromatography tandem mass spectrometry. Journal of Veterinary Science 14, 107-114. doi: 10.4142/jvs.2013.14.2.107.
128. Snelling,W. J., Lin, Q., Moore, J. E., Millar, B. C., Tosini, F., Pozio, E., Dooley, J. S. G. and Lowery, C. J. (2007). Proteomics analysis and protein expression during sporozoite excystation of Cryptosporidium parvum (Coccidia, Apicomplexa). Molecular & Cellular Proteomics 6, 346-355. doi: 10.1074/mcp.M600372-MCP200.
129. Spano, F., Puri, C., Ranucci, L., Putignani, L. and Crisanti, A. (1997). Cloning of the entire COWP gene of Cryptosporidium parvum and ultrastructural localization of the protein during sexual parasite development. Parasitology 114, 427-437.
130. Spano, F., Putignani, L., Naitza, S., Puri, C., Wright, S. and Crisanti, A. (1998). Molecular cloning and expression analysis of a Cryptosporidium parvum gene encoding a new member of the thrombospondin family. Molecular and Biochemical Parasitology 92, 147-162. doi: 10.1016/S0166-6851(97)00243-0.
131. Spielmann, T., Montagna, G. N., Hecht, L. and Matuschewski, K. (2012). Molecular make-up of the Plasmodium parasitophorous vacuolar membrane. International Journal of Medical Microbiology 302, 179-186. doi: 10.1016/j.ijmm.2012.07.011.
132. Striepen, B., Pruijssers, A. J. P., Huang, J., Li, C., Gubbels, M.-J., Umejiego, N. N., Hedstrom, L. and Kissinger, J. C. (2004). Gene transfer in the evolution of parasite nucleotide biosynthesis. Proceedings of the National Academy of Sciences USA 101, 3154-3159. doi: 10.1073/pnas.0304686101.
133. Strong, W. B. and Nelson, R. G. (2000). Preliminary profile of the Cryptosporidium parvum genome: an expressed sequence tag and genome survey
134. Strong, W. B., Gut, J. and Nelson, R. G. (2000). Cloning and sequence analysis of a highly polymorphic Cryptosporidium parvum gene encoding a 60-kilodalton glycoprotein and characterization of its 15-and 45-kilodalton zoite surface antigen products. Infection and Immunity 68, 4117-4134.
135. Sturbaum, G. D., Jost, B. H. and Sterling, C. R. (2003). Nucleotide changes within three Cryptosporidium parvum surface protein encoding genes differentiate genotype I from genotype II isolates. Molecular and Biochemical Parasitology 128, 87-90. doi: 10.1016/S0166-6851(03)00017-3.
136. Sturbaum, G. D., Schaefer, D. A., Jost, B. H., Sterling, C. R. and Riggs, M.W. (2008). Antigenic differences within the Cryptosporidium hominis and Cryptosporidium parvum surface proteins P23 and GP900 defined by monoclonal antibody reactivity. Molecular and Biochemical Parasitology 159, 138-141. doi: 10.1016/j.molbiopara.2008.02.009.
137. Takashima, Y., Xuan, X., Kimata, I., Iseki, M., Kodama, Y., Nagane, N., Nagasawa, H., Matsumoto, Y., Mikami, T. and Otsuka, H. (2003). Recombinant bovine herpesvirus-1 expressing p23 protein of Cryptosporidium parvum induces neutralizing antibodies in rabbits. Journal of Parasitology 89, 276-282. doi: 10.1645/0022-3395 (2003)089[0276:RBHEPP]2.0.CO;2.
138. Templeton, T. J., Lancto, C. A., Vigdorovich, V., Liu, C., London, N. R., Hadsall, K. Z. and Abrahamsen, M. S. (2004a). The Cryptosporidium oocyst wall protein is a member of a multigene family and has a homolog in Toxoplasma. Infection and Immunity 72, 980-987.
139. Templeton, T. J., Iyer, L. M., Anantharaman, V., Enomoto, S., Abrahante, J. E., Subramanian, G. M., Hoffman, S. L., Abrahamsen, M. S. and Aravind, L. (2004b). Comparative analysis of apicomplexa and genomic diversity in eukaryotes. Genome Research 14, 1686-1695. doi: 10.1101/gr.2615304.
140. Templeton, T. J., Enomoto, S., Chen, W.-J., Huang, C.-G., Lancto, C. A., Abrahamsen, M. S. and Zhu, G. (2010). A genomesequence survey for Ascogregarina taiwanensis supports evolutionary affiliation but metabolic diversity between a Gregarine and Cryptosporidium. Molecular Biology and Evolution 27, 235-248. doi:10.1093/molbev/msp226.
141. Tilley, M., Upton, S. J., Fayer, R., Barta, J. R., Chrisp, C. E., Freed, P. S., Blagburn, B. L., Anderson, B. C. and Barnard, S. M. (1991). Identification of a 15-kilodalton surface glycoprotein on sporozoites of Cryptosporidium parvum. Infection and Immunity 59, 1002-1007.
142. Tosini, F., Agnoli, A., Mele, R., Gomez Morales, M. A. and Pozio, E. (2004). A new modular protein of Cryptosporidium parvum, with ricin B and LCCL domains, expressed in the sporozoite invasive stage. Molecular and Biochemical Parasitology 134, 137-147. doi: 10.1016/j.molbiopara.2003.11.014.
143. Umejiego, N. N., Li, C., Riera, T., Hedstrom, L. and Striepen, B. (2004). Cryptosporidium parvum IMP dehydrogenase: identification of functional, structural, and dynamic properties that can be exploited for drug design. Journal of Biological Chemistry 279, 40320-40327. doi: 10.1074/jbc.M407121200.
144. Valigurová, A., Jirků, M., Koudela, B., Gelnar, M., Modrý, D. and Slapeta, J. (2008). Cryptosporidia: epicellular parasites embraced by the host cell membrane. International Journal for Parasitology 38, 913-922. doi:10.1016/j.ijpara.2007.11.003.
145. Wanyiri, J.W., O'Connor, R., Allison, G., Kim, K., Kane, A., Qiu, J., Plaut, A. G. and Ward, H. D. (2007). Proteolytic processing of the Cryptosporidium glycoprotein gp40/15 by human furin and by a parasitederived furin-like protease activity. Infection and Immunity 75, 184-192. doi: 10.1128/IAI.00944-06.
146. Wanyiri, J.W., Techasintana, P., O'Connor, R. M., Blackman, M. J., Kim, K. and Ward, H. D. (2009). Role of CpSUB1, a subtilisin-like protease, in Cryptosporidium parvum infection in vitro. Eukaryotic Cell 8, 470-477. doi: 10.1128/EC.00306-08.
147. Wetzel, D. M., Schmidt, J., Kuhlenschmidt, M. S., Dubey, J. P. and Sibley, L. D. (2005). Gliding motility leads to active cellular invasion by Cryptosporidium parvum sporozoites. Infection and Immunity 73, 5379-5387. doi: 10.1128/IAI.73.9.5379-5387.2005.
148. Widmer, G. and Lee, Y. (2010). Comparison of single-and multilocus genetic diversity in the protozoan parasites Cryptosporidium parvum and C. hominis. Applied and Environmental Microbiology 76, 6639-6644. doi: 10.1128/AEM.01268-10.
149. Widmer, G., Lee, Y., Hunt, P., Martinelli, A., Tolkoff,M. and Bodi, K. (2012). Comparative genome analysis of two Cryptosporidium parvum isolates with different host range. Infection, Genetics and Evolution 12, 1213-1221. doi: 10.1016/j.meegid.2012.03.027.
150. Wyatt, C. R. and Perryman, L. E. (2000). Detection of mucosally delivered antibody to Cryptosporidium parvum p23 in infected calves. Annals of the New York Academy of Sciences 916, 378-387.
151. Wyatt, C. R., Brackett, E. J., Mason, P. H., Savidge, J. and Perryman, L. E. (2000). Excretion patterns of mucosally delivered antibodies to p23 in Cryptosporidium parvum infected calves. Veterinary Immunology and Immunopathology 76, 309-317.
152. Xiao, D., Yin, C., Zhang, Q., Li, J., Gong, P., Li, S., Zhang, G., Gao, Y. and Zhang, X. (2011). Selection and identification of a new adhesion protein of Cryptosporidium parvum from a cDNA library by ribosome display. Experimental Parasitology 129, 183-189. doi: 10.1016/j.exppara.2011.06.004.
153. Xu, P., Widmer, G., Wang, Y., Ozaki, L. S., Alves, J. M., Serrano, M. G., Puiu, D., Manque, P., Akiyoshi, D., Mackey, A. J., Pearson, W. R., Dear, P. H., Bankier, A. T., Peterson, D. L., Abrahamsen, M. S., Kapur, V., Tzipori, S. and Buck, G. A. (2004). The genome of Cryptosporidium hominis. Nature 431, 1107-1112. doi: 10.1038/nature02977.
154. Yamagishi, J., Wakaguri, H., Sugano, S., Kawano, S., Fujisaki, K., Sugimoto, C., Watanabe, J., Suzuki, Y., Kimata, I. and Xuan, X. (2011). Construction and analysis of full-length cDNA library of Cryptosporidium parvum. Parasitology International 60, 199-202. doi:10.1016/j.parint.2011.03.001.
155. Yao, L., Yin, J., Zhang, X., Liu, Q., Li, J., Chen, L., Zhao, Y., Gong, P. and Liu, C. (2007). Cryptosporidium parvum: identification of a new surface adhesion protein on sporozoite and oocyst by screening of a phage-display cDNA library. Experimental Parasitology 115, 333-338. doi: 10.1016/j.exppara.2006.09. 018.
156. Yarlett, N., Wu, G., Waters,W. R., Harp, J. A., Wannemuehler, M. J., Morada, M., Athanasopoulos, D., Martinez, M. P., Upton, S. J., Marton, L. J. and Frydman, B. J. (2007). Cryptosporidium parvum spermidine/spermine N1-acetyltransferase exhibits different characteristics from the host enzyme. Molecular and Biochemical Parasitology 152, 170-180. doi: 10.1016/j.molbiopara. 2007.01.004.
157. Yu, Q., Li, J., Zhang, X., Gong, P., Zhang, G., Li, S. and Wang, H. (2010). Induction of immune responses in mice by a DNAvaccine encoding Cryptosporidium parvum Cp12 and Cp21 and its effect against homologous oocyst challenge. Veterinary Parasitology 172, 1-7. doi: 10.1016/j.vetpar.2010.04.036.
158. Zeng, B. and Zhu, G. (2006). Two distinct oxysterol binding proteinrelated proteins in the parasitic protist Cryptosporidium parvum (Apicomplexa). Biochemical and Biophysical Research Communications 346, 591-599. doi: 10.1016/j.bbrc.2006.05.165.
159. Zeng, B., Cai, X. and Zhu, G. (2006). Functional characterization of a fatty acyl-CoA-binding protein (ACBP) from the apicomplexan Cryptosporidium parvum. Microbiology 152, 2355-2363. doi: 10.1099/mic.0.28944-0.
160. Zhang, H., Guo, F., Zhou, H. and Zhu, G. (2012a). Transcriptome analysis reveals unique metabolic features in the Cryptosporidium parvum oocysts associated with environmental survival and stresses. BMC Genomics 13, 647. doi: 10.1186/1471-2164-13-647.
161. Zhang, H., Guo, F. and Zhu, G. (2012b). Involvement of host cell integrin a2 in Cryptosporidium parvum infection. Infection and Immunity 80, 1753-1758. doi: 10.1128/IAI.05862-11.
162. Zhu, G. (2004). Current progress in the fatty acid metabolism in Cryptosporidium parvum. Journal of Eukaryotic Microbiology 51, 381-388.
163. Zhu, G. and Keithly, J. S. (1997). Molecular analysis of a P-type ATPase from Cryptosporidium parvum. Molecular and Biochemical Parasitology 90, 307-316. doi: 10.1016/S0166-6851(97)00168-0.
164. Zhu, G., LaGier, M. J., Stejskal, F., Millership, J. J., Cai, X. and Keithly, J. S. (2002). Cryptosporidium parvum: the first protist known to encode a putative polyketide synthase. Gene 298, 79-89.
165. Zhu, G., Li, Y., Cai, X., Millership, J. J., Marchewka, M. J. and Keithly, J. S. (2004). Expression and functional characterization of a giant Type I fatty acid synthase (CpFAS1) gene from Cryptosporidium parvum. Molecular and Biochemical Parasitology 134, 127-135.