Molecular cloning and expression of a gene encoding Cryptosporidium parvum glycoproteins gp40 and gp15

Infection and Immunity

Volumn 68, Issue 7, 2000, Pages 4108-4116

  Cevallos, Ana Maria ^a   Zhang, Xiaoping ^a   Waldor, Matthew K ^a   Jaison, Smitha ^a   Zhou, Xiaoyin ^b   Tzipori, Saul ^a,c   Neutra, Marian R ^b   Ward, Honorine D ^a,c,d  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

^c TUFTS UNIVERSITY   (United States)

^d TUFTS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; GLYCOPROTEIN GP 15; GLYCOPROTEIN GP 40; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM PARVUM; DIARRHEA; HOST PARASITE INTERACTION; HUMAN; HUMAN CELL; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; CACO-2 CELLS; CELL ADHESION; CLONING, MOLECULAR; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM PARVUM; DNA PRIMERS; GENE EXPRESSION; GENES, PROTOZOAN; GLYCOPROTEINS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; NEUTRALIZATION TESTS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTOZOAN PROTEINS;

EID: 0033933752     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.7.4108-4116.2000     Document Type: Article

References (44)

1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Barnes, D. A., A. Bonnin, J. X. Huang, L. Gousset, J. Wu, J. Gut, P. Doyle, J. F. Dubremetz, H. Ward, and C. Petersen. 1998. A novel multi-domain mucin-like glycoprotein of Cryptosporidium parvum mediates invasion. Mol. Biochem. Parasitol. 96:93-110.
3. Blackman, M., and A. Holder. 1992. Secondary processing of the Plasmodium falciparum merozoite surface protein (MSP-1) by a calcium-dependent membrane bound serine protease: shedding of MSP-133 as a non-covalently associated complex with other fragments of MSP-1. Mol. Biochem. Parasitol. 50:307-316.
4. Bradley, P. J., and J. C. Boothroyd. 1999. Identification of the pro-mature processing site of Toxoplasma ROP1 by mass spectrometry. Mol. Biochem. Parasitol. 100:103-109.
5. 4a.Eng, J., A. L. McCormack, and J. R. Yates III. 1994. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database. J. Am. Soc. Mass Spectrom. 5:976-989.
6. Forney, J. R., S. Yang, and M. C. Healey. 1996. Protease activity associated with excystation of Cryptosporidium parvum oocysts. J. Parasitol. 82:889-892.
7. Fricker, C. R., and J. H. Crabb. 1998. Water-borne cryptosporidiosis: detection methods and treatment options. Adv. Parasitol. 40:241-278.
8. Griffiths, J. K. 1998. Human cryptosporidiosis: epidemiology, transmission, clinical disease, treatment, and diagnosis. Adv. Parasitol. 40:37-85.
9. Guerrant, R. L. 1997. Cryptosporidiosis: an emerging, highly infectious threat. Emerg. Infect. Dis. 3:51-57.
10. Gut, J., and R. G. Nelson. 1994. Cryptosporidium parvum sporozoites deposit trails of 11A5 antigen during gliding locomotion and shed 11A5 antigen during invasion of MDCK cells in vitro. J. Eukaryot. Microbiol. 41:42S-43S.
11. Hamer, D. H., H. D. Ward, S. Tzipori, M. E. Pereira, J. P. Alroy, and G. T. Keusch. 1994. Attachment of Cryptosporidium parvum sporozoites to MDCK cells in vitro. Infect. Immun. 62:2208-2213.
12. Hansen, J. E., O. Lund, N. Tolstrup, A. A. Gooley, K. L. Williams, and S. Brunak. 1998. NetOglyc: prediction of mucin type O-glycosylation sites based on sequence context and surface accessibility. Glycoconj. J. 15:115-130.
13. Holder, A. A., and R. R. Freeman. 1984. The three major antigens on the surface of Plasmodium falciparum merozoites are derived from a single high molecular weight precursor. J. Exp. Med. 160:624-629.
14. Holder, A. A., M. J. Lockyer, K. G. Odink, J. S. Sandhu, V. Riveros-Moreno, S. C. N. Hillman, L. S. Davi, M. L. V. Tizard, R. T. Schwarz, and R. R. Freeman. 1985. Primary structure of the precursor to the three major surface antigens of Plasmodium falciparum merozoites. Nature 317:270-273.
15. Holder, A. A., J. S. Sandhu, Y. Hillman, L. S. Davey, S. C. Nicholls, H. Cooper, and M. J. Lockyer. 1987. Processing of the precursor to the major merozoite surface antigens of Plasmodium falciparum. Parasitology 94:199-208.
16. Horton, P., and K. Nakai. 1997. Better prediction of protein cellular localization sites with the k nearest neighbors classifier. Ismb 5:147-152.
17. Jenkins, M. C., and R. Fayer. 1995. Cloning and expression of cDNA encoding an antigenic Cryptosporidium parvum protein. Mol. Biochem. Parasitol. 71:149-152.
18. Jenkins, M. C., and C. Petersen. 1997. Molecular biology of Cryptosporidium, p. 225-232. In R. Fayer (ed.). Cryptosporidium and cryptosporidiosis. CRC Press, Boca Raton, Fla.
19. Joe, A., D. H. Hamer, M. A. Kelley, M. E. Pereira, G. T. Keusch, S. Tzipori, and H. D. Ward. 1994. Role of a Gal/GalNAc-specific sporozoite surface lectin in Cryptosporidium parvum-host cell interaction. J. Eukaryot. Microbiol. 41:44S.
20. Joe, A., R. Verdon, S. Tizpori, G. T. Keusch, and H. D. Ward. 1998. Attachment of Cryptosporidium parvum sporozoites to human intestinal epithelial cells. Infect. Immun. 66:3429-3432.
21. Khramtsov, N. V., B. Oppert, B. A. Montelone, and S. J. Upton. 1997. Sequencing, analysis and expression in Escherichia coli of a gene encoding a 15-kDa Cryptosporidium parvum protein. Biochem. Biophys. Res. Commun. 230:164-166.
22. Langer, R., and M. Riggs. 1999. Cryptosporidium parvum apical complex glycoprotein CSL contains a sporozoite ligand for intestinal epithelial cells. Infect. Immun. 67:5282-5291.
23. Manger, I. D., A. B. Hehl, and J. C. Boothroyd. 1998. The surface of Toxoplasma tachyzoites is dominated by a family of glycosylphosphatidylinositol-anchored antigens related to SAG1. Infect. Immun. 66:2237-2244.
24. McConville, M. J., and A. J. Ferguson. 1993. The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes. Biochem. J. 249:305-324.
25. Nesterenko, M. V., M. Tilley, and S. J. Upton. 1995. A metallo-dependent cysteine proteinase of Cryptosporidium parvum associated with the surface of sporozoites. Microbios 83:77-88.
26. Nesterenko, M. V., K. Woods, and S. J. Upton. 1999. Receptor/ligand interactions between Cryptosporidium parvum and the surface of the host cell. Biochim. Biophys. Acta 1454:165-173.
27. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10:1-6.
28. Okhuysen, P. C., H. L. DuPont, C. R. Sterling, and C. L. Chappell. 1994. Arginine aminopeptidase, an integral membrane protein of the Cryptosporidium parvum sporozoite. Infect. Immun. 62:4667-4670.
29. Pearson, W. R. 1990. Rapid and sensitive sequence comparison with FASTP and FASTA. Methods Enzymol. 183:63-98.
30. Riggs, M. W., A. L. Stone, P. A. Yount, R. C. Langer, M. J. Arrowood, and D. L. Bentley. 1997. Protective monoclonal antibody defines a circumsporozoite-like glycoprotein exoantigen of Cryptosporidium parvum sporozoites and merozoites. J. Immunol. 158:1787-1795.
31. Schenkman, S., M. A. Ferguson, N. Heise, M. L. de Almeida, R. A. Mortara, and N. Yoshida. 1993. Mucin-like glycoproteins linked to the membrane by glycosylphosphatidylinositol anchor are the major acceptors of sialic acid in a reaction catalyzed by trans-sialidase in metacyclic forms of Trypanosoma cruzi. Mol. Biochem. Parasitol. 59:293-303.
32. Schroeder, A. A., C. E. Lawrence, and M. S. Abrahamsen. 1999. Differential mRNA display cloning and characterization of a Cryptosporidium parvum gene expressed during intracellular development. J. Parasitol. 85:213-220.
33. Soldati, D., A. Lassen, J. F. Dubremetz, and J. C. Boothroyd. 1998. Processing of Toxoplasma ROP1 protein in nascent rhoptries. Mol. Biochem. Parasitol. 96:37-48.
34. Spano, F., L. Putignani, S. Naitza, C. Puri, S. Wright, and A. Crisanti. 1998. Molecular cloning and expression analysis of a Cryptosporidium parvum gene encoding a new member of the thrombospondin family. Mol. Biochem. Parasitol. 92:147-162.
35. Tilley, M., S. J. Upton, R. Fayer, J. R. Barta, C. E. Chrisp, P. S. Freed, B. L. Blagburn, B. C. Anderson, and S. M. Barnard. 1991. Identification of a 15-kilodalton surface glycoprotein on sporozoites of Cryptosporidium parvum. Infect. Immun. 59:1002-1007.
36. Tomavo, S., R. T. Schwarz, and J. F. Dubremetz. 1989. Evidence for glycosylphosphatidylinositol anchoring of Toxoplasma gondii major surface antigens. Mol. Cell. Biol. 9:4576-4580.
37. Tzipori, S., and J. K. Griffiths. 1998. Natural history and biology of Cryptosporidium parvum. Adv. Parasitol. 40:6-36.
38. Tzipori, S., W. Rand, J. Griffiths, G. Widmer, and J. Crabb. 1994. Evaluation of an animal model system for cryptosporidiosis: therapeutic efficacy of paromomycin and hyperimmune bovine colostrum-immunoglobulin. Clin. Diagn. Lab. Immunol. 1:450-463.
39. Van Beers, E. H., R. H. Al, E. H. Rings, A. W. Einerhand, J. Dekker, and H. A. Buller. 1995. Lactase and sucrase-isomaltase gene expression during Caco-2 cell differentiation. Biochem. J. 308:769-775.
40. Verdon, R., G. T. Keusch, S. Tzipori, S. A. Grubman, D. M. Jefferson, and H. D. Ward. 1997. An in vitro model of infection of human biliary epithelial cells by Cryptosporidium parvum. J. Infect. Dis. 175:1268-1272.
41. Ward, H. D., and A. M. Cevallos. 1998. Cryptosporidium: molecular basis of host-parasite interaction. Adv. Parasitol. 40:151-185.
42. Ward, H. D., A. V. Kane, E. Ortega-Barria, G. T. Keusch, and M. E. Pereira. 1990. Identification of developmentally regulated Giardia lamblia cyst antigens using GCSA-1, a cyst- specific monoclonal antibody. Mol. Microbiol. 4:2095-2102.
43. Weltzin, R. L., P. Lucia-Zandris, P. Michetti, B. N. Fields, J. P. Kraehnbuhl, and M. R. Neutra. 1989. Binding and transepithelial transport of immunoglobulins by intestinal M cells: demonstration using monoclonal IgA against enteric viral proteins. J. Cell Biol. 108:1673-1685.
44. Yada, T., Y. Totoki, M. Ishikawa, K. Asai, and K. Nakai. 1998. Automatic extraction of motifs represented in the hidden Markov model from a number of DNA sequences. Bioinformatics 14:317-325.