Therapeutic alpha-interferons protein: Structure, production, and biosimilar

Preparative Biochemistry and Biotechnology

Volumn 45, Issue 2, 2015, Pages 109-127

  El Baky, Nawal Abd ^a   Redwan, Elrashdy M ^a,b  

^a KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

^b Universities and Research Institutes District   (Egypt)

Author keywords

alpha interferons; antiproliferative activity; antiviral therapy; hepatitis C virus treatment; immunotherapeutic drug; interferon stimulated genes

Indexed keywords

ALPHA INTERFERON; BIOSIMILAR AGENT; IFNA2 PROTEIN, HUMAN;

CHEMISTRY; HEPACIVIRUS; HEPATITIS C, CHRONIC; HUMAN; PROTEIN ENGINEERING; PROTEIN TERTIARY STRUCTURE;

BIOSIMILAR PHARMACEUTICALS; HEPACIVIRUS; HEPATITIS C, CHRONIC; HUMANS; INTERFERON-ALPHA; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY;

EID: 84906546058     PISSN: 10826068     EISSN: 15322297     Source Type: Journal    
DOI: 10.1080/10826068.2014.907175     Document Type: Article

References (130)

1. Isaacs, A.; Lindenmann, J. Virus Interference. 1. The Interferon. Proc. Roy. Soc. B 1957, 147, 258-273.
2. Merigan, T.C. Interferons of Mice and Men. N. Engl. J. Med. 1967, 276, 913-920.
3. Rubinstein, M.; Rubinstein, S.; Familletti, P.C.; Miller, R.S.; Waldmann, A.; Pestka, S. Human Leukocyte Interferon Purified to Homogeneity. Science 1978, 202, 1289-1290.
4. Nagata, S.; Mantei, N.; Weissmann, C. The Structure of One of the Eight or More Distinct Chromosomal Genes for Human Interferon-Alpha. Nature 1980, 287, 401-408.
5. Weissman, C.; Nagata, S.; Boll, W. Structure and Expression of Human Alpha Interferon Genes. Mol. Cell. Biol. 1982, 25, 295-326.
6. Pestka, S.; Baron, S. Definition and Classification of the Interferons. Methods Enzymol. 1981, 78, 3-14.
7. Pestka, S. Interferon From 1981 to 1986. Methods Enzymol. 1986, 119, 3-14.
8. Pestka, S.; Langer, J.A.; Zoon, K.C.; Samuel, C.E. Interferons and Their Actions. Annu. Rev. Biochem. 1987, 56, 727-777.
9. Bach, E.A.; Aguet, M.; Schreiber, R.D. The IFN Gamma Receptor: A Paradigm for Cytokine Receptor Signaling. Annu. Rev. Immunol. 1997, 15, 563-591.
10. Capon, D.J.; Shepard, H.M.; Geodedel, D.V. Two Distinct Families of Human and Bovine Interferon-Alpha Genes Are Coordinately Expressed and Encode Functional Polypeptides. Mol. Cell Biol. 1985, 5, 768-779.
11. Feinstein, S.I.; Mory, Y.; Chernajovsky, Y.; Maroteaux, L.; Ni, U.; Lavie, U.; Revel, M. Family of Human Alpha-Interferon Like Sequences. Mol. Cell. Biol. 1985, 5, 510-517.
12. Hauptmann, R.; Swetly, P. A Novel Class of Human Type I Interferons. Nucleic Acids Res. 1985, 13, 4739-4749.
13. Adolf, G.R.; Maurer-Fogy, I.; Kalsner, I.; Cantell, K. Purification and Characterization of Natural Human Interferon Omega. 1. Two Alternative Cleavage Sites For The Signal Peptidase. J. Biol. Chem. 1990, 265, 9290-9295.
14. Shirono, H.; Kono, K.; Koga, J.; Hayashi, S.; Matsuo, A.; Hiratani, H. Existence and Unique N-Terminal Sequence of Alpha II (Omega) Interferon in Natural Leukocyte Interferon Preparation. Biochem. Biophys. Res. Commun. 1990, 168, 16-21.
15. Nagata, S.; Taira, H.; Hall, A.; Johnsrud, L.; Streuli, M.; Ecsödi, J.; Boll, W.; Cantell, K.; Weissmann, C. Synthesis in E. coli of a Polypeptide With Human Leukocyte Interferon Activity. Nature 1980, 284, 316-20.
16. Streuli, M.; Nagata, S.; Weissman, C. At Least Three Human Type αInterferons: Structure of α2. Science 1980, 209, 1343-1347.
17. Rehberg, E.; Kelder, B.; Hoal, E.G.; Pestka, S. Specific Molecular Activities of Recombinant and Hybrid Leukocyte Interferons. J. Biol. Chem. 1982, 257, 11497-11502.
18. Goeddel, D.V.; Leung, D.W.; Dull, T.J.; Gross, M.; Lawn, R.M.; McCandliss, R.; Seeburg, P.H.; Ullrich, A.; Yelverton, E.; Gray, P.W. The Structure of Eight Distinct Cloned Human Leukocyte Interferon cDNAs. Nature 1981, 290, 20-26.
19. Baron, S.; Tyring, S.K.; Fleischmann, R.; Coppenhaver, D.H.; Niesel, D.W.; Klimpel, G.R.; Stanton, G.J. The Interferons: Mechanisms of Action and Medical Applications. J. Am. Med. Assoc. 1991, 266, 1375-1383.
20. Weinstock-Guttman, B.; Ransohoff, R.M.; Kinkel, R.P.; Rudick, R.A. The Interferons: Biological Effects, Mechanisms of Action and Use in Multiple Sclerosis. Ann. Neurol. 1995, 37, 7-15.
21. Redwan, el-RM. Cumulative Updating of Approved Biopharmaceuticals. Hum. Antibodies 2007, 16, 137-158.
22. Redwan, el-R.M. Animal-Derived Pharmaceutical Proteins. J. Immunoassay Immunochem. 2009, 30, 262-290.
23. Harris, J.M.; Martin, N.E.; Modi, M. Pegylation: A Novel Process for Modifying Pharmacokinetics. Clin. Pharmacokinet. 2001, 40, 539-551.
24. Blatt, L.M.; Davis, J.M.; Klein, S.B.; Taylor, M.W. The Biologic Activity and Molecular Characterization of a Novel Synthetic Interferon-Alpha Species, Consensus Interferon. J. Interferon Cytokine Res. 1996, 16, 489-499.
25. Alton, K.; Stabinsky, Y.; Richards, R.; Ferguson, B.; Goldstein, L.; Altrock, B.; Miller, L.; Stebbing, N. Production, Characterization and Biological Effects of Recombinant DNA Derived Human IFN-α and IFN-γ Analogs. In The Biology of the Interferon System. Elsevier: Amsterdam, The Netherlands, 1983, pp. 119-128.
26. Klein, M.L.; Bartley, T.D.; Lai, P.H.; Lu, H.S. Structural Characterization of Recombinant Consensus Interferon-α. J. Chromatogr. 1988, 454, 205-215.
27. Ozes, O.N.; Reiter, Z.; Klein, S.; Blatt, L.M.; Taylor, M.W. A Comparison of Interferon-Con1 With Natural Recombinant Interferons Alpha: Antiviral, Antiproliferative, and Natural Killer-Inducing Activities. J. Interferon Res. 1992, 12, 55-59.
28. Sen, G.C.; Lengyel, P. The Interferon System. A Bird's Eye View of its Biochemistry. J. Biol. Chem. 1992, 267, 5017-5020.
29. Genin, P.; Vaccaro, A.; Civas, A. The Role of Differential Expression of Human Interferon-α Genes in Antiviral Immunity. Cytokine Growth Factor Rev. 2009, 20, 283-295.
30. Runkel, L.; Pfeffer, L.; Lewerenz, M.; Monneron, D.; Yang, C.H.; Murti, A.; Pellegrini, S.; Goelz, S.; Uze, G.; Mogensen, K. Differences in Activity Between Alpha and Beta Type I Interferons Explored by Mutational Analysis. J. Biol. Chem. 1998, 273, 8003-8008.
31. Wetzel, R. Assignment of the Disulphide Bonds of Leukocyte Interferon. Nature 1981, 289, 606-607.
32. Miller, D.L.; Kung, H.F.; Pestka, S. Crystallization of Recombinant Human Leukocyte Interferon A. Science 1982, 215, 689-690.
33. Matsuda, S.; Senda, T.; Itoh, S.; Kawano, G.; Mizuno, H.; Mitsui, Y. New Crystal Form of Recombinant Murine Interferon-Beta. J. Biol. Chem. 1989, 264, 13381-13382.
34. Klaus, W.; Gsell, B.; Labhardt, A.M.; Wipf, B.; Senn, H. The Three-Dimensional High Resolution Structure of Human Interferon Alpha-2a Determined by Heteronuclear NMR Spectroscopy in Solution. J. Mol. Biol. 1997, 274, 661-675.
35. Radhakrishnan, R.; Walter, L.J.; Hruza, A.; Reichert, P.; Trotta, P.P.; Nagabhushan, T.L.; Walter, M.R. Zinc Mediated Dimer of Human Interferon-Alpha 2b Revealed by X-Ray Crystallography. Structure 1996, 4, 1453-1463.
36. Redwan, el-RM.; Matarm, S.M.; Serour, I.A. Recombinant Human J-Chain: Fix the Protein Aggregations and Yield Maximize. Hum. Antibodies 2006, 15, 95-102.
37. Redwan, el-RM.; Matar, S.M.; El-Aziz, G.A.; Serour, E.A. Synthesis of the Human Insulin Gene: Protein Expression, Scaling Up and Bioactivity. Prep. Biochem Biotechnol. 2008, 38, 24-39.
38. Nyman, T.A.; Kalkkinen, N.; Tolo, H.; Helin, J. Structural Characterization of N-Linked and O-Linked Oligosaccharides Derived From Interferon-α2b and Interferon-α14c Produced by Sendai-Virus-Induced Human Peripheral Blood Leukocytes. Eur. J. Biochem. 1998a, 253, 485-493.
39. Nyman, T.A.; Tolo, H.; Parkkinen, J.; Kalkkinen, N. Identification of Nine Interferon-Alpha Subtypes Produced by Sendai Virus-Induced Human Peripheral Blood Leucocytes. Biochem. J. 1998b, 329, 295-302.
40. Pestka, S. The Human Interferons-From Protein Purification and Sequence to Cloning and Expression in Bacteria: Before, Between, and Beyond. Arch. Biochem. Biophys. 1983, 221, 1-37.
41. Goeddel, D.V.; Yelverton, E.; Ullrich, A.; Heyneker, H.L.; Miozzari, G.; Holmes, W.; Seeburg, P.; Dull, H.T.; May, L.; Stebbing, N.; Crea, R.; Maeda, S.; McCandliss, R.; Sloma, A.; Tabor, J.M.; Gross, M.; Familletti, P.C.; Pestka, S. Human Leukocyte Interferon Produced by E. coli Is Biologically Active. Nature 1980, 287, 411-416.
42. Baron, E.; Narrola, S. From Cloning to Commercial Realization: Human aInterferon. Biotechnology 1990, 10, 179-184.
43. Swaminathan, S.; Khanna, N. Affinity Purification of Recombinant Interferon-Alpha on a Mimetic Ligand Adsorbent. Protein Express. Purif. 1999, 15, 236-242.
44. Babu, K.R.; Swaminathan, S.; Marten, S.; Khanna, N.; Rinas, U. Production of Interferon-Alpha in High Cell Density Cultures of Recombinant Escherichia coli and Its Single Step Purification From Refolded Inclusion Body Proteins. Appl. Microbiol. Biotechnol. 2000, 53, 655-660.
45. Srivastava, P.; Bhattacharaya, P.; Pandey, G.; Mukherjee, K.J. Overexpression and Purification of Recombinant Human Interferon Alpha-2b in Escherichia coli. Protein Express. Purif. 2005, 41, 313-322.
46. rg(AGG/AGA) in High-Cell-Density Cultures of Escherichia coli. Biotechnol. Bioprocess Eng. 2001, 6, 301-305.
47. Mohammed, Y.; EL-Baky, N.A.; Redwan, E.M. Expression, Purification, and Characterization of Recombinant Human Consensus Interferon-Alpha in Escherichia coli Under λPL Promoter. Prep. Biochem. Biotechnol. 2012, 42, 426-447.
48. Lim, H.K.; Jung, K.H.; Park, D.H.; Chung, S.I. Production Characteristics of Interferon-Alpha Using an L-Arabinose Promoter System in a High-Cell-Density Culture. Appl. Microbiol. Biotechnol. 2000, 53, 201-208.
49. BAD Promoter. Biochemistry (Mosc.) 2012, 77, 1210-1219.
50. Villaverde, A.; Carrio, M.M. Protein Aggregation in Recombinant Bacteria: Biological Role of Inclusion Bodies. Biotechnol. Lett. 2003, 25, 1385-1395.
51. Middelberg, A. Preparative Protein Refolding. Trend Biotechnol. 2002, 20, 437.
52. Kane, J.F. Effects of Rare Codon Clusters on High-Level Expression of Heterologous Proteins in Escherichia coli. Curr. Opin. Biotechnol. 1995, 6, 494-500.
53. AGA/AGG. Biochem. Biophys. Res. Commun. 2002, 296, 1303-1309.
54. Redwan, R.M. The Optimal Gene Sequence for Optimal Protein Expression in Escherichia coli: Principle Requirements (A Review Article). Arab J. Biotechnol. 2006, 9, 493-510.
55. Tan, J.S.; Ling, T.C.; Mustafa, S.; Tam, Y.J.; Ramanan, R.N.; Ariff, A.B. An Integrated Bioreactor-Expanded Bed Adsorption System for the Removal of Acetate to Enhance the Production of Alpha-Interferon-2b by Escherichia coli. Process Biochem. 2013, 48, 551-558.
56. Lin, Y.K.; Ooi, C.W.; Tan, J.S.; Show, P.L.; Ariff, A.; Ling, T.C. Recovery of Human Interferon Alpha-2b From Recombinant Escherichia coli Using Alcohol=Salt-Based Aqueous Two-Phase Systems. Separat. Purif. Technol. 2013, 120, 362-366.
57. Breitling, R.; Gerlach, D.; Hartmann, M.; Behnke, D. Secretory Expression in Escherichia coli and Bacillus subtilis of Human Interferon αGenes Directed by Staphylokinase Signals. Mol. Gen. Genet. 1989, 217, 384-391.
58. Pulido, D.; Vara, J.A.; Jimenez, A. Cloning and Expression in Biologically Active Form of the Gene for Human Interferon Alpha 2 in Streptomyces lividans. Gene 1986, 45, 167-174.
59. Hao, Y.; Chu, J.; Wang, Y.; Zhang, S.; Zhuang, Y. Expression and Aggregation of Recombinant Human Consensus Interferon-α Mutant by Pichia pastoris. Biotechnol. Lett. 2006, 28, 905-909.
60. Salunkhe, S.; Soorapaneni, S.; Prasad, K.S.; Raiker, V.A.; Padmanabhan, S. Strategies to Maximize Expression of Rightly Processed Human Interferon α2b in Pichia pastoris. Protein Express. Purif. 2010, 71, 139-146.
61. Rossmann, C.; Sharp, N.; Allen, G.; Gewert, D. Expression and Purification of Recombinant, Glycosylated Human Interferon Alpha 2b in Murine Myeloma NSo cells. Protein Express. Purif. 1996, 7, 335-342.
62. Maeda, S.; Kawai, T.; Obinata, M.; Fujiwara, H.; Horichi, T.; Sacki, Y.; Furusawa, M. Production of Human Alpha-Interferon in Silkworm Using a Baculovirus Vector. Nature 1985, 315, 592-594.
63. Pestka, S.; Mcinnes, J.; Havell, E.A.; Vilcek, J. Cell-Free Synthesis of Human Interferon. Proc. Natl. Acad. Sci. USA 1975, 72, 3898-3901.
64. Reynolds, F.H.; Zicha, M.; Pitha, P.M. Biochemical Characterization of Human Interferon Activity Produced in a Cell-Free Protein Synthesis System. Abstr. Annu. Meet. Am. Soc. Microbiol. 1975, S339, 270.
65. El-Baky, N.A.; Omar, S.H.; Redwan, E.M. The Anti-Cancer Activity of Human Consensus Interferon-Alpha Synthesized in Cell-Free System. Protein Express. Purif. 2011, 80, 61-67.
66. Ortaldo, J.R.; Mason, A.; Rehberg, E.; Moschera, J.; Kelder, B.; Pestka, S.; Herberman, R.B. Effects of Recombinant and Hybrid Recombinant Human Leukocyte Interferons on Cytotoxic Activity of Natural Killer Cells. J. Biol. Chem. 1983, 258, 15011-15015.
67. Fellous, M.; Nir, U.; Wallach, D.; Merlin, G.; Rubenstein, M.; Revel, M. Interferon-Dependent Induction of mRNA for the Major Histocompatibility Antigens in Human Fibroblasts and Lymphoblastoid Cells. Proc. Natl. Acad. Sci. USA 1982, 79, 3082-3086.
68. Brassard, D.L.; Grace, M.J.; Bordens, R.W. Interferon-α as an Immunotherapeutic Protein. J. Leukocyte Biol. 2002, 71, 565-581.
69. Wenner, C.A.; Guler, M.L.; Macatonia, S.E.; O'Garra, A.; Murphy, K.M. Roles of IFN-Gamma and IFN-Alpha in IL-12-Induced T Helper Cell-1 Development. J. Immunol. 1996, 156, 1442-1447.
70. Von Hoegen, P. Synergistic Role of Type I Interferons in the Induction of Protective Cytotoxic T Lymphocytes. Immunol. Lett. 1995, 47, 157-162.
71. Lenschow, D.J.; Giannakopoulos, N.V.; Gunn, L.J.; Johnston, C.; O'Guin, A.K.; Schmid, R.E. Identification of Interferon-Stimulated Gene 15 as an Antiviral Molecule During Sindbis Virus Infection In Vivo. J. Virol. 2005, 79, 13974-13983.
72. Sadler, A.J.; Williams, B.R. Interferon-Inducible Antiviral Effectors. Nat. Rev. Immunol. 2008, 8, 559-568.
73. Komatsu, T.; Takeuchi, K.; Yokoo, J.; Tanaka, Y.; Gotoh, B. Sendai Virus Blocks Alpha Interferon Signaling to Signal Transducers and Activators of Transcription. J. Virol. 2000, 74, 2477-2480.
74. Gotoh, B.; Takeuchi, K.; Komatsu, T.; Yokoo, J. The STAT2 Activation Process Is a Crucial Target of Sendai Virus C Protein for the Blockade of Alpha Interferon Signaling. J. Virol. 2003, 77, 3360-3370.
75. Der, S.D.; Zhou, A.; Williams, B.R.; Silverman, R.H. Identification of Genes Differentially Regulated by Interferon Alpha, Beta, or Gamma Using Oligonucleotide Arrays. Proc. Natl. Acad. Sci. USA 1998, 95, 15623-15628.
76. Ji, X.; Cheung, R.; Cooper, S.; Li, Q.; Greenberg, H.B.; He, X.S. Interferon Alpha Regulated Gene Expression in Patients Initiating Interferon Treatment for Chronic Hepatitis C. Hepatology 2003, 37, 610-621.
77. Pichlmair, A.; Reis e Sousa, C. Innate Recognition of Viruses. Immunity 2007, 27, 370-383.
78. Liu, K.; Liao, X.; Zhou, B.; Yao, H.; Fan, S.; Chen, P.; Miao, D. Porcine Alpha Interferon Inhibits Japanese Encephalitis Virus Replication by Different ISGs In Vitro. Res. Vet. Sci. 2013, 95, 950-956.
79. Sentsui, H.; Takami, R.; Nishimori, T.; Murakami, K.; Yokoyama, T.; Yokomizo, Y. Anti-Viral Effect of Interferon-α on Bovine Viral Diarrhea Virus. J. Vet. Med. Sci. 1998, 60, 1329-1333.
80. Koyama, T.; Sakamoto, N.; Tanabe, Y.; Nakagawa, M.; Itsui, Y.; Takeda, Y.; Kakinuma, S.; Sekine, Y.; Maekawa, S.; Yanai, Y.; Kurimoto, M.; Watanabe, M. Divergent Activities of Interferon-Alpha Subtypes Against Intracellular Hepatitis C Virus Replication. Hepatol. Res. 2006, 34, 41-49.
81. Finter, N.B. Interferons and Interferon Inducers. In The Assay and Standardization of Interferon and Interferon Inducers. Finter, N.B., Ed. North-Holland: Amsterdam, the Netherlands, 1973; pp. 135-169.
82. Caserman, S.; Menart, V.; Gaines, R.; Williams, S.; Meager, A. Thermal Stability of the WHO International Standard of Interferon Alpha 2b (IFN-α2b): Application of New Reporter Gene Assay for IFN-α2b Potency Determinations. J. Immunol. Methods 2007, 319, 6-12.
83. Cantell, K.; Hirvonen, S.; Mogensen, K.E.; Pyhala, L. Human Leukocyte Interferon: Production, Purification, Stability and Animal Experiments. In The Production and Use of Interferon for the Treatment and Prevention of Human Virus Infections. In Vitro Monograph. Waymouth, C., Ed. Tissue Culture Association: Rockville, MD, 1974; Vol. 3, pp. 35-38.
84. Bajcetić, M.; Zigon, N.; Samardzić, R.; Beleslin, D.B. Alpha Interferons-New Therapeutic Modalities. Med. Pregl. 1998, 51, 135-139.
85. Brunetto, M.R.; Oliveri, F.; Coco, B.; Leandro, G.; Colombatto, P.; Monti Gorin, J.; Bonino, F. The Outcome of Anti-HBe Positive Chronic Hepatitis B in Alpha Interferon Treated and Untreated Patients: A Long Term Cohort Study. J. Hepatol. 2002, 36, 263-270.
86. Freid, M.W.; Hoofnagle, J.H. Therapy of Hepatitis C. Semin. Liver Dis. 1995, 15, 82-91.
87. Badgwell, B.; Lesinski, G.B.; Magro, C.; Abood, G.; Skaf, A.; Carson, W. The Antitumor Effects of Interferon-Alpha Are Maintained in Mice Challenged With a STAT1-Deficient Murine Melanoma Cell Line. J. Surg. Res. 2004, 116, 129-136.
88. Eto, M.; Kamba, T.; Miyake, H.; Fujisawa, M.; Kamai, T.; Uemura, H.; Tsukamoto, T.; Azuma, H.; Matsubara, A.; Nishimura, K.; Nakamura, T.; Ogawa, O.; Naito, S. STAT3 Polymorphism Can Predict the Response to Interferon-α Therapy in Patients With Metastatic Renal Cell Carcinoma. Eur. Urol. 2013, 63, 745-752.
89. Magyari, M.; Søndergaard, H.B.; Sellebjerg, F.; Sørensen, P.S. Preserved In Vivo Response to Interferon-Alpha in Multiple Sclerosis Patients With Neutralising Antibodies Against Interferon-Beta (REPAIR Study). Multiple Sclerosis Relat. Disorders 2013, 2, 141-146.
90. Belardelli, F.; Ferrantini, M.; Proietti, E.; Kirkwood, J.M. Interferon-Alpha in Tumor Immunity and Immunotherapy. Cytokine Growth Factor Rev. 2002, 13, 119-134.
91. Albert, M.L.; Sauter, B.; Bhardwaj, N. Dendritic Cells Acquire Antigen From Apoptotic Cells and Induce Class I-Restricted CTLs. Nature 1998, 392, 86-9.
92. Reguiga, M.B.; Bouquet, C.; Farinotti, R.; Bonhomme-Faivre, L. Interferon-Alpha Improves Docetaxel Antitumoral and Antimetastatic Efficiency in Lewis Lung Carcinoma Bearing Mice. Life Sci. 2012, 91, 843-851.
93. Gutterman, J.U. Cytokine Therapeutics: Lessons From Interferon-α. Proc. Natl. Acad. Sci. USA 1994, 91, 1198-1205.
94. Foster, G.R.; Carman, W.F.; Thomas, H.C. Replication of Hepatitis B and Delta Viruses: Appearance of Viral Mutants. Sem. Liver Dis. 1991, 112, 121-127.
95. Hayashi, J.; Furusyo, N.; Ariyama, I.; Sawayama, Y.; Etoh, Y.; Kashiwagi, S. A Relationship Between the Evolution of Hepatitis C Virus Variants, Liver Damage, and Hepatocellular Carcinoma in Patients With Hepatitis C Viremia. J. Infect. Dis. 2000, 181, 1523-1527.
96. Tong, M.J.; Blatt, L.M.; Tong, L.T.; Sayadzadeh, K. A Long-Term Retreatment in Chronic Hepatitis C Patients Who Were Non-Responders to an Initial Course of Interferon-Alpha 2b. J. Viral Hepat. 1998, 5, 323-331.
97. Hassan, M.M.; Zaghloul, A.S.; El-Serag, H.B.; Soliman, O.; Patt, Y.Z.; Chappell, C.L.; Beasley, R.P.; Hwang, L.Y. The Role of Hepatitis C in Hepatocellular Carcinoma-A Case Control Study Among Egyptian Patients. J. Clin. Gastroenterol. 2001, 33, 123-126.
98. El-Zanaty, F.; Way, A. Egypt Demographic and Health Survey 2008. Egyptian Ministry of Health. El-Zanaty and Associates and Macro International: Cairo, Egypt, 2009; p. 431.
99. El-Zayadi, A.; Simmonds, P.; Dabbous, H.; Prescott, L.; Selim, O.; Ahdy, A. Response to Interferon-α of Egyptian Patients Infected With Hepatitis C Virus Genotype 4. J. Viral Hepatitis 1996, 3, 261-264.
100. El-Zayadi, A.; Selim, O.; Haddad, S.; Simmonds, P.; Hamdy, H.; Badran, H.M.; Shawky, S. Combination Treatment of Interferon α-2b and Ribavirin in Comparison to Interferon Monotherapy in Treatment of Chronic Hepatitis C Genotype 4 Patients. Ital. J. Gastroenterol. Hepatol. 1999, 31, 472-475.
101. Shepherd, J.; Waugh, N.; Hewitson, P. Combination Therapy (Interferon Alfa and Ribavirin) in the Treatment of Chronic Hepatitis C: A Rapid and Systematic Review. Health Technol. Assess. 2000, 4, 1-67.
102. Stylianou, E.; Aukrust, P.; Bendtzen, K.; Muller, F.; Froland, S.S. Interferons and Interferon (IFN)-Inducible Protein 10 During Highly Active Anti-Retroviral Therapy (HAART)-Possible Immunosuppressive Role of IFN-Alpha in HIV Infection. Clin. Exp. Immunol. 2000, 119, 479-485.
103. Pitha, P.M. Multiple Effects of Interferon on the Replication of Human Immunodeficiency Virus Type 1. Antiviral Res. 1994, 24, 205-219.
104. Goujon, C.; Malim, M.H. Characterization of the Alpha Interferon-Induced Post Entry Block to HIV-1 Infection in Primary Human Macrophages and T Cells. J. Virol. 2010, 84, 9254-9266.
105. Lotrich, F.E.; Sears, B.; McNamara, R.K. Anger Induced by Interferon-Alpha is Moderated by Ratio of Arachidonic Acid to Omega-3 Fatty Acids. J. Psychosomatic Res. 2013, 75, 475-483.
106. Papari, A.C.; Cozaru, G.C.; Papari, A. Anxiety and Depression in Patients With Chronic Hepatitis C Receiving Interferon-Alpha Therapy. Eur. Psychiatry 2013, 28(Suppl. 1), 1.
107. Mayszczak, K.M.; Frydecka, D.A.; Pawowski, T.; Inglot, M. Functional Polymorphism in the Interleukin-6 Gene (IL-6 RS1800795), and Depression Induced by Interferon-Alpha and Ribavirin During the HCV Treatment. Eur. Psychiatry 2013, 28(Suppl. 1), 1.
108. Borden, E.C.; Parkinson, D. Interferons: Effectiveness, Toxicities, and Costs. Ann. Intern. Med. 1996, 125, 614-616.
109. Zeuzem, S.; Feinman, S.V.; Rasenack, J.; Heathcote, E.J.; Lai, M.Y.; Gane, E. Peginterferon Alfa-2a in Patients With Chronic Hepatitis C. N. Engl. J. Med. 2000, 343, 1666-1672.
110. Lindsay, K.L.; Trepo, C.; Heintges, T.; Shiffman, M.L.; Gordon, S.C.; Hoefs, J.C.; Schiff, E.R.; Goodman, Z.D.; Laughlin, M.; Yao, R.; Albrecht, J.K. A Randomized, Double-Blind Trial Comparing Pegylated Interferon Alfa-2b to Interferon alfa-2b as Initial Treatment for Chronic Hepatitis C. Hepatology 2001, 34, 395-403.
111. Manns, M.P.; McHutchinson, J.G.; Gordon, S.C.; Rustgi, V.K.; Shiffman, M.; Reindollar, R.; Goodman, Z.D.; Koury, K.; Ling, M.; Albrecht, J.K. Peginterferon Alfa-2b Plus Ribavirin Compared With Interferon Alfa-2b Plus Ribavirin for Initial Treatment of Chronic Hepatitis C: A Randomized Trial. Lancet 2001, 358, 958-965.
112. Kamal, S.M.; El tawil, A.A.; Nakano, T.; He, Q.; Rasenack, J.; Hakam, S.A. Peginterferon {Alpha}-2b and Ribavirin Therapy in Chronic Hepatitis C Genotype 4: Impact of Treatment Duration and Viral Kinetics on Sustained Virological Response. Gut 2005, 54, 858-866.
113. El Makhzangy, H.; Esmat, G.; Said, M.; Raziky, M.E.; Shouman, S.; Refai, R. Response to Pegylated Interferon Alfa-2a and Ribavirin in Chronic Hepatitis C Genotype 4. J. Med. Virol. 2009, 81, 1576-1583.
114. Allweiss, L.; Volz, T.; Lütgehetmann, M.; Giersch, K.; Bornscheuer, T.; Lohse, A.W.; Petersen, J.; Ma, H.; Klumpp, K.; Fletcher, S.P.; Dandri, M. Immune Cell Responses Are Not Required to Induce Substantial Hepatitis B Virus Antigen Decline During Pegylated Interferon Alpha Administration. J. Hepatol. 2013, doi:http://dx.doi.org/10.1016/j.jhep.2013.10.021
115. Chapplain, J.M.; Bellissant, E.; Guyader, D.; Molina, J.M.; Poizot-Martin, I.; Perré, P.; Pialoux, G.; Turlin, B.; Mouchel, C.; Renault, A.; Michelet, C. The Effects of a Maintenance Therapy With Peg-Interferon Alpha-2a on Liver Fibrosis in HIV=HCV Co-Infected Patients: A Randomized Controlled Trial. J. Infect. 2013, 67, 313e321.
116. Subramanian, G.M.; Fiscella, M.; Lamousé-Smith, A.; Zeuzem, S.; Mchutchison, J.G. Albinterferon Alpha-2b: A Genetic Fusion Protein for the Treatment of Chronic Hepatitis C. Nat. Biotechnol. 2007, 25, 1411-1419.
117. Grace, M.; Youngster, S.; Gitlin, G.; Sydor, W.; Xie, L.; Westreich, L.; Jacobs, S.; Brassard, D.; Bausch, J.; Bordens, R. Structural and Biologic Characterization of Pegylated Recombinant IFN-Alpha2b. J. Interferon Cytokine Res. 2001, 21, 1103-1115.
118. Foser, S.; Schacher, A.; Weyer, K.A.; Brugger, D.; Dietel, E.; Marti, S.; Schreitmuller, T. Isolation, Structural Characterization, and Antiviral Activity of Positional Isomers of Monopegylated Interferon Alpha-2a (PEGASYS). Protein Expression Purif. 2003, 30, 78-87.
119. Van Der Auwera, P.; Platzer, E.; Xu, Z.X.; Schulz, R.; Feugeas, O.; Capdeville, R.; Edwards, D. Pharmacodynamics and Pharmacokinetics of Single Doses of Subcutaneous Pegylated Human G-CSF Mutant (Ro 25-8315) in Healthy Volunteers: Comparison With Single and Multiple Daily Doses of Filgrastim. Am. J. Hematol. 2001, 66, 245-251.
120. Heathcote, J.; James, S.; Mullen, K.D.; Hauser, S.C.; Rosenblate, H.; Albert, D.G. Chronic Hepatitis C Virus Patients With Breakthroughs During Interferon Treatment Can Successfully Be Retreated With Consensus Interferon. J. Hepatol. 1999, 30, 562-566.
121. Da Silva, L.C.; Bassit, L.; Ono-Nita, S.K.; Pinho, J.R.; Nishiya, A.; Madruga, C.L.; Carrilho, F.J. High Rate of Sustained Response to Consensus Interferon Plus Ribavirin in Chronic Hepatitis C Patients Resistant to Alpha-Interferon and Ribavirin: A Pilot Study. J. Gastroenterol. 2002, 37, 732-736.
122. Cornberg, M.; Hadem, J.; Herrmann, E.; Schuppert, F.; Schmidt, H.H.; Reiser, M.; Marschal, O.; Steffen, M.; Manns, M.P.; Wedemeyer, H. Treatment With Consensus Interferon (CIFN) Plus Ribavirin in Non-Responder Patients With Chronic Hepatitis C: A Randomized Open-Label Pilot Study. J. Hepatol. 2006, 44, 291-301.
123. Kaiser, S.; Hass, H.; Lutze, B.; Gregor, M. Higher Susceptibility of Peginterferon Alfa-2a Versus Peginterferon 2b Non-Responder Patients With Chronic Hepatitis C to Re-Treatment With Consensus Interferon Daily Dosing and Ribavirin [Abstract]. J. Gastroenterol. 2006b, 130, S1061.
124. Kaiser, S.; Hass, H.; Lutze, B.; Gregor, M. Comparison of Daily Consensus Interferon Versus Peginterferon Alfa-2a Extended Therapy of 72 Weeks for Peginterferon=Ribavirin Relapse Patients With Chronic Hepatitis C [abstr.]. J. Gastroenterol. 2006a, 130, S1060.
125. Fitzgerald-Bocarsly, P. Natural Interferon-Alpha Producing Cells: The Plasmacytoid Dendritic Cells. Biotechniques 2002, 16-20, 22, 24-29.
126. Rönnblom, L.; Alm, G.V. The Natural Interferon-Alpha Producing Cells in Systemic Lupus Erythematosus. Hum. Immunol. 2002, 63, 1181-1193.
127. Rönnblom, L.; Eloranta, M.L.; Alm, G.V. Role of Natural Interferon-Alpha Producing Cells (Plasmacytoid Dendritic Cells) in Autoimmunity. Autoimmunity 2003, 36, 463-472.
128. Hagberg, N.; Berggren, O.; Leonard, D.; Weber, G.; Bryceson, Y.T.; Alm, G.V.; Eloranta, M.L.; Rönnblom, L. IFN-α Production by Plasmacytoid Dendritic Cells Stimulated With RNA-Containing Immune Complexes Is Promoted by NK Cells Via MIP-1β and LFA-1. J. Immunol. 2011, 186, 5085-5094.
129. Timothy, N.B.; Clark, D.N.; Salloum, R.; Poole, B.D. Interferon Alpha in Systemic Lupus Erythematosus. J. Biomed. Biotechnol. 2010, article ID 948364.
130. Kirou, K.A.; Gkrouzman, E. Anti-Interferon Alpha Treatment in SLE. Clin. Immunol. 2013, 148, 303-312.