Affinity purification of recombinant interferon-α on a mimetic ligand adsorbent

Protein Expression and Purification

Volumn 15, Issue 2, 1999, Pages 236-242

  Swaminathan, Sathyamangalam ^a   Khanna, Navin ^a  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

Author keywords

Downstream processing; Dye affinity chromatography; Interferon ; Purification; Refolding

Indexed keywords

ALPHA INTERFERON; PROTEIN FOLDING; PROTEIN PURIFICATION; RECOMBINANT PROTEIN;

ENCEPHALOMYOCARDITIS VIRUS;

EID: 0033102463     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.1017     Document Type: Article

References (20)

1. Gutterman J. U. Cytokine therapeutics: Lessons from interferon-α Proc. Natl. Acad. Sci. USA. 91:1994;1198-1205.
2. Physician's Desk Reference. 1993;. p. 2194-2201.
3. Remington: The Science and Practice of Pharmacy. 1995;Mack Publishing.
4. Edge M. D., Greene A. R., Heathcliffe G. R., Moore V. E., Faulkner N. J., Camble R., Petter N. N., Trueman P., Schuch W., Hennam J. Chemical synthesis of a human interferon-alpha 2 gene and its expression inEscherichia coli. Nucleic Acids Res. 11:1983;6419-6435.
5. Hitzeman R. A., Hagie F. E., Levine H. L., Goeddel D. V., Ammerer G., Hall B. D. Expression of a human gene for interferon in yeast. Nature. 293:1981;717-722.
6. Thatcher D. R., Panayotatos N. Purification of recombinant human IFN-α2. Methods Enzymol. 119:1986;166-177.
7. Tarnowski S. J., Roy S. K., Liptak R. A., Lee D. K., Ning R. Y. Large-scale purification of recombinant human leukocyte interferons. Methods Enzymol. 119:1986;153-165.
8. Cho, J. M. Park, Y. W. Park, S. J. Bae, T. O. Chang, H. J. 1993, Recombinant gene coding for human alpha interferon and expression vector thereof.
9. Ettlin, U. Hochuli, E. Schacher, A. Weyer, K. 1995, Process for producing alpha-interferon.
10. Lowe C. R., Pearson J. C. Affinity chromatography on immobilized dyes. Methods Enzymol. 104:1984;97-113.
11. Lowe C. R., Burton S. J., Burton N. P., Alderton W. K., Pitts J. M., Thomas J. A. Designer dyes: 'Biomimetic' ligands for the purification of pharmaceutical proteins by affinity chromatography. Trends Biotechnol. 10:1992;442-448.
12. Stewart D. J., Purvis D. R., Pitts J. M., Lowe C. R. Development of an enzyme-linked immunosorbent assay for C. I. Reactive Blue 2 and its application to a comparison of the stability and performance of perfluorocarbon support with other immobilized C. I. Reactive Blue 2 affinity adsorbents. J. Chromatogr. 623:1992;1-14.
13. Lowe C. R., Burton S. J., Burton N., Stewart D. J., Purvis D. R., Pitfield I., Eapen S. New developments in affinity chromatography. J. Mol. Recognit. 3:1990;117-122.
14. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:1976;248-254.
15. Arora D., Khanna N. Method for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. J. Biotechnol. 52:1996;127-133.
16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
17. De Bernardez Clark E. Refolding of recombinant proteins. Curr. Opin. Biotechnol. 9:1998;157-163.
18. Buchner J., Pastan I., Brinkmann U. A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies. Anal. Biochem. 205:1992;263-270.
19. LEscherichia coli. Methods Enzymol. 119:1986;366-375.
20. Hannig G., Makrides S. C. Strategies for optimizing heterologous protein expression inEscherichia coli. Trends Biotechnol. 16:1998;54-60.