Expression, purification, and characterization of recombinant human consensus interferon-alpha in escherichia coli under λpL promoter

Preparative Biochemistry and Biotechnology

Volumn 42, Issue 5, 2012, Pages 426-447

  Mohammed, Y ^a   El Baky, N A ^a   Redwan, E M ^a,b  

^a Universities and Research centers District   (Egypt)

^b KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

Author keywords

codon optimization; Escherichia coli; human consensus interferon alpha; inclusion bodies; synthetic gene

Indexed keywords

ALPHA INTERFERON; ALPHA2A INTERFERON; PRIMER DNA; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CELL INCLUSION; CELL SURVIVAL; CHEMISTRY; CODON; CONSENSUS SEQUENCE; CULTURE MEDIUM; DRUG EFFECT; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HEAT; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PLASMID; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN ENGINEERING; PROTEIN REFOLDING; TUMOR CELL LINE;

AMINO ACID SEQUENCE; CELL LINE, TUMOR; CELL SURVIVAL; CODON; CONSENSUS SEQUENCE; CULTURE MEDIA; DNA PRIMERS; ESCHERICHIA COLI; GENETIC VECTORS; HOT TEMPERATURE; HUMANS; INCLUSION BODIES; INTERFERON-ALPHA; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; PROTEIN ENGINEERING; PROTEIN REFOLDING; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 84865650460     PISSN: 10826068     EISSN: 15322297     Source Type: Journal    
DOI: 10.1080/10826068.2011.637600     Document Type: Article

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