In situ protein secondary structure determination in ice: Raman spectroscopy-based process analytical tool for frozen storage of biopharmaceuticals

Journal of Pharmaceutical Sciences

Volumn 103, Issue 8, 2014, Pages 2287-2295

  Roessl, Ulrich ^a,b   Leitgeb, Stefan ^a   Pieters, Sigrid ^c   De Beer, Thomas ^d   Nidetzky, Bernd ^a,b  

^a RESEARCH CENTER PHARMACEUTICAL ENGINEERING GMBH   (Austria)

^b GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^c FREE UNIVERSITY OF BRUSSELS   (Belgium)

^d GHENT UNIVERSITY   (Belgium)

Author keywords

freezing thawing; multivariate analysis; partial least squares; processing; protein structure; proteins; Raman spectroscopy; stability; unit operations

Indexed keywords

ALCOHOL DEHYDROGENASE; AMYLASE; BETA LACTOGLOBULIN; BOVINE SERUM ALBUMIN; ICE; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2; INSULIN; LACTATE DEHYDROGENASE; LYSOZYME; PEPSIN A; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR; RIBONUCLEASE A; STABILIZING AGENT; TRYPSIN; PROTEIN;

ARTICLE; CIRCULAR DICHROISM; CONTROLLED STUDY; CRYOPRESERVATION; DRUG STORAGE; FREEZE THAWING; LIQUID; MONITORING; PROTEIN DENATURATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; RAMAN SPECTROMETRY; ANIMAL; CHEMISTRY; FREEZING; HUMAN; MULTIVARIATE ANALYSIS; PROCEDURES; REGRESSION ANALYSIS;

ANIMALS; FREEZING; HUMANS; ICE; LEAST-SQUARES ANALYSIS; MULTIVARIATE ANALYSIS; PROTEIN STRUCTURE, SECONDARY; PROTEINS; SPECTRUM ANALYSIS, RAMAN;

EID: 84905085501     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.24072     Document Type: Article

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