Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: Fermentation strategies and impact on downstream processing

Journal of Biotechnology

Volumn 186, Issue , 2014, Pages 119-127

  Gonçalves, Geisa A L ^a,b   Prather, Kristala L J ^a,c   Monteiro, Gabriel A ^a,b   Carnes, Aaron E ^d   Prazeres, Duarte M F ^a,b  

^a IBB Institute for Biotechnology and Bioengineering Centre for Biological and Chemical Engineering Department of Bioengineering Instituto Superior Técnico Universidade de Lisboa ^*

^b INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^c Massachusetts Institute of Technology   (United States)

^d NATURE TECHNOLOGY CORPORATION   (United States)

Author keywords

DNA vaccine; Escherichia coli; Fermentation; Plasmid biopharmaceutical

Indexed keywords

BATCH CELL CULTURE; BIOMOLECULES; DNA; ESCHERICHIA COLI; GLUCOSE; LIQUID CHROMATOGRAPHY; VACCINES; FERMENTATION;

DNA VACCINE; DOWNSTREAM-PROCESSING; FED-BATCH FERMENTATION; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; INDUSTRIAL FERMENTATION; PENTOSE PHOSPHATE PATHWAY; PLASMID BIOPHARMACEUTICAL; PLASMID BIOPHARMACEUTICALS; GENE KNOCKOUT; PLASMID DNA;

FERMENTATION; ESCHERICHIA COLI;

ACETIC ACID; ACETYL COENZYME A; GLUCOSE; GLYCEROL; PLASMID DNA; TRICARBOXYLIC ACID; DNA VACCINE; ESCHERICHIA COLI PROTEIN; GLUCOSE 6 PHOSPHATE ISOMERASE; PGI PROTEIN, E COLI;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BIOMASS PRODUCTION; CARBON SOURCE; CELL DENSITY; CELL LYSATE; DOWNSTREAM PROCESSING; ESCHERICHIA COLI; FED BATCH CULTURE; FED BATCH FERMENTATION; GEL PERMEATION CHROMATOGRAPHY; GENE INACTIVATION; GENOTYPE; GLUCOSE INTAKE; GLUCOSE METABOLISM; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; LARGE SCALE PRODUCTION; PENTOSE PHOSPHATE CYCLE; PRECIPITATION; PRIORITY JOURNAL; BATCH CELL CULTURE; BIOMASS; BIOREACTOR; FERMENTATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; PLASMID; PROCEDURES; AGAR GEL ELECTROPHORESIS; DNA PURIFICATION; FERMENTATION TECHNIQUE; NONHUMAN;

BATCH CELL CULTURE TECHNIQUES; BIOMASS; BIOREACTORS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERMENTATION; GENE KNOCKOUT TECHNIQUES; GLUCOSE; GLUCOSE-6-PHOSPHATE ISOMERASE; PLASMIDS; VACCINES, DNA;

EID: 84904721409     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.06.008     Document Type: Article

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