Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography

Biotechnology and Bioengineering

Volumn 68, Issue 5, 2000, Pages 576-583

  Diogo, M M ^a,b   Queiroz, J A ^b   Monteiro, G A ^a,c   Martins, S A M ^a,c   Ferreira, G N M ^a   Prazeres, D M F ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b UNIVERSITY OF BEIRA INTERIOR   (Portugal)

^c UNIVERSIDADE LUSÓFONA DE HUMANIDADES E TECNOLOGIAS   (Portugal)

Author keywords

Cystic fibrosis; Gene therapy; Hydrophobic interaction chromatography; Nucleic acids; Plasmid purification

Indexed keywords

AMMONIUM COMPOUNDS; DNA; HYDROPHOBIC CHROMATOGRAPHY; PRECIPITATION (CHEMICAL); PURIFICATION; RNA;

GENE THERAPY; HYDROPHOBIC INTERACTION CHROMATOGRAPHY;

GENETIC ENGINEERING;

1,4 BUTANEDIOL; AMMONIUM ACETATE; LIPOPOLYSACCHARIDE; PLASMID DNA; PLASMID VECTOR; SEPHAROSE; SINGLE STRANDED DNA;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CHROMATOGRAPHY; CYSTIC FIBROSIS; DERIVATIZATION; DNA SUPERCOILING; GENE THERAPY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION;

CHROMATOGRAPHY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; ENDOTOXINS; ESCHERICHIA COLI; GENE THERAPY; GENETIC VECTORS; HUMANS; PLASMIDS; QUALITY CONTROL;

EID: 0034608587     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000605)68:5<576::AID-BIT13>3.0.CO;2-5     Document Type: Article

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