Development of new plasmid DNA vaccine vectors with R1-based replicons

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Bower, Diana M ^a   Prather, Kristala L J ^a  

^a Massachusetts Institute of Technology   (United States)

Author keywords

DNA vaccines; Plasmid DNA; R1 replicon; Runaway replication

Indexed keywords

DNA VACCINE; MESSENGER RNA; PLASMID DNA; REPLICATION FACTOR A;

ARTICLE; CELL CULTURE; DNA VECTOR; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION; MUTANT; NONHUMAN; PHENOTYPE; PROTEIN EXPRESSION; REPLICON; START CODON; TEMPERATURE;

DNA HELICASES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; PLASMIDS; TEMPERATURE; TRANS-ACTIVATORS; VACCINES, DNA;

ESCHERICHIA COLI;

EID: 84864812821     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-107     Document Type: Article

References (31)

1. Kutzler MA, Weiner DB. DNA vaccines: ready for prime time?. Nat Rev Genet 2008, 9:776-788. 10.1038/nrg2432, 18781156.
2. Moore JP, Klasse PJ, Dolan MJ, Ahuja SK. AIDS/HIV. A STEP into darkness or light?. Science 2008, 320:753-755. 10.1126/science.1154258, 18467578.
3. Saade F, Petrovsky N. Technologies for enhanced efficacy of DNA vaccines. Expert Rev Vaccines 2012, 11:189-209. 10.1586/erv.11.188, 22309668.
4. Sardesai NY, Weiner DB. Electroporation delivery of DNA vaccines: Prospects for success. Curr Opin Immunol 2011, 23:421-429. 10.1016/j.coi.2011.03.008, 3109217, 21530212.
5. Lin-Chao S, Chen W, Wong T. High copy number of the pUC plasmid results from a Rom/Rop-suppressible point mutation in RNA II. Mol Microbiol 1992, 6:3385-3393. 10.1111/j.1365-2958.1992.tb02206.x, 1283002.
6. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng 2009, 103:1129-1143. 10.1002/bit.22347, 2735187, 19408315.
7. Soubrier F, Cameron B, Manse B, Somarriba S, Dubertret C, Jaslin G, Jung G, Caer CL, Dang D, Mouvault JM, Scherman D, Mayaux JF, Crouzet J. pCOR: a new design of plasmid vectors for nonviral gene therapy. Gene Ther 1999, 6:1482-1488. 10.1038/sj.gt.3300968, 10467373.
8. Soubrier F, Laborderie B, Cameron B. Improvement of pCOR plasmid copy number for pharmaceutical applications. Appl Microbiol Biotechnol 2005, 66:683-688. 10.1007/s00253-004-1729-9, 15349701.
9. Uhlin BE, Molin S, Gustafsson P, Nordström K. Plasmids with temperature-dependent copy number for amplification of cloned genes and their products. Gene 1979, 6:91-106. 10.1016/0378-1119(79)90065-9, 383579.
10. Nordström K. Plasmid R1-Replication and its control. Plasmid 2006, 55:1-26. 10.1016/j.plasmid.2005.07.002, 16199086.
11. Givskov M, Stougaard P, Light J, Molin S. Identification and characterization of mutations responsible for a runaway replication phenotype of plasmid R1. Gene 1987, 57:203-211. 10.1016/0378-1119(87)90123-5, 3692168.
12. Ansorge MB, Kula M. Production of recombinant L-leucine dehydrogenase from Bacillus cereus in pilot scale using the runaway replication system E. coli[pIET98]. Biotechnol Bioeng 2000, 68:557-562. 10.1002/(SICI)1097-0290(20000605)68:5<557::AID-BIT10>3.0.CO;2-J, 10797242.
13. Hoffmann I, Widstrom J, Zeppezauer M, Nyman PO. Overproduction and large-scale preparation of deoxyuridine triphosphate nucleotidohydrolase from Escherichia coli. Eur J Biochem 1987, 164:45-51. 10.1111/j.1432-1033.1987.tb10990.x, 3030754.
14. Morino T, Morita M, Seya K, Sukenaga Y, Kato K, Nakamura T. Construction of a runaway vector and its use for a high-level expression of a cloned human superoxide dismutase gene. Appl Microbiol Biotechnol 1988, 28:170-175.
15. Kidwell J, Valentin H, Dennis D. Regulated expression of the Alcaligenes eutrophus pha biosynthesis genes in Escherichia coli. Appl Environ Microbiol 1995, 61:1391-1398. 167396, 7747959.
16. Remaut E, Tsao H, Fiers W. Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication. Gene 1983, 22:103-113. 10.1016/0378-1119(83)90069-0, 6305768.
17. Prather KJ, Sagar S, Murphy J, Chartrain M. Industrial scale production of plasmid DNA for vaccine and gene therapy: Plasmid design, production, and purification. Enzyme Microb Technol 2003, 33:865-883.
18. We{ogonek}grzyn G. Replication of plasmids during bacterial response to amino acid starvation. Plasmid 1999, 41:1-16. 10.1006/plas.1998.1377, 9887302.
19. Mizutani S, Iijima S, Kobayashi T. Fed-batch culture of Escherichia coli harboring a runaway-replication plasmid. J Chem Eng Jpn 1986, 19:111-116.
20. Light J, Riise E, Molin S. Transcription and its regulation in the basic replicon region of plasmid R1. Mol Gen Genet 1985, 198:503-508. 10.1007/BF00332947, 2409430.
21. Nordström K, Uhlin BE. Runaway-replication plasmids as tools to produce large quantities of proteins from cloned genes in bacteria. Nat Biotechnol 1992, 10:661-666.
22. Kozak M. Regulation of translation via mRNA structure in prokaryotes and eukaryotes. Gene 2005, 361:13-37.
23. Masai H, Arai K. RepA protein- and oriR-dependent initiation of R1 plasmid replication: Identification of a rho-dependent transcription terminator required for cis-action of repA protein. Nucleic Acids Res 1988, 16:6493-6514. 10.1093/nar/16.14.6493, 338310, 3041379.
24. Giraldo-Suárez R, Fernández-Tresguerres E, Díaz-Orejas R, Malki A, Kohiyama M. The heat-shock DnaK protein is required for plasmid R1 replication and it is dispensable for plasmid ColE1 replication. Nucleic Acids Res 1993, 21:5495-5499. 10.1093/nar/21.23.5495, 310592, 8265367.
25. Gross CA. Function and regulation of the heat shock proteins. Escherichia coli and Salmonella: Cellular and molecular biology 1996, 1382-1399. Washington, DC: ASM Press, Neidhardt FC, 2.
26. Klinman DM, Klaschik S, Tross D, Shirota H, Steinhagen F. FDA guidance on prophylactic DNA vaccines: Analysis and recommendations. Vaccine 2010, 28:2801-2805. 10.1016/j.vaccine.2009.11.025, 2847045, 19941989.
27. Pedelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol 2006, 24:79-88. 10.1038/nbt1172, 16369541.
28. Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res 1987, 15:8125-8148. 10.1093/nar/15.20.8125, 306349, 3313277.
29. Bower DM, Lee KS, Ram RJ, Prather KLJ. Fed-batch microbioreactor platform for scale down and analysis of a plasmid DNA production process. Biotechnol Bioeng 2012, 109:1976-1986. 10.1002/bit.24498, 22422584.
30. Livak K, Schmittgen T. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 2001, 25:402-408. 10.1006/meth.2001.1262, 11846609.
31. Zor T, Selinger Z. Linearization of the Bradford protein assay increases its sensitivity: Theoretical and experimental studies. Anal Biochem 1996, 236:302-308. 10.1006/abio.1996.0171, 8660509.