Development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip LAMP) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals

Journal of Microbiological Methods

Volumn 104, Issue , 2014, Pages 26-35

  Zhou, Qian Jin ^a   Wang, Lei ^b   Chen, Jiong ^a   Wang, Rui Na ^a   Shi, Yu Hong ^a   Li, Chang Hong ^a   Zhang, De Min ^a   Yan, Xiao Jun ^a   Zhang, Yan Jun ^c  

^a NINGBO UNIVERSITY   (China)

^b CapitalBio Corporation   (China)

^c ZHEJIANG PROVINCIAL CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

Author keywords

Aquaculture; Loop mediated isothermal amplification; Microfluidic chip; Multiplex detection; Pathogenic bacteria; Real time detection

Indexed keywords

BACTERIAL DNA; DNA 16S; DNA TOPOISOMERASE (ATP HYDROLYSING) B; GENOMIC DNA; HEMOLYSIN; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN D; OUTER MEMBRANE PROTEIN K; OUTER MEMBRANE PROTEIN U; RNA 16S; SIGMA FACTOR RPON; TOLC PROTEIN; UNCLASSIFIED DRUG; SEA WATER;

ANALYTIC METHOD; ANIMAL TISSUE; AQUACULTURE; AQUATIC SPECIES; ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CONTINUOUS PROCESS; CONTROLLED STUDY; EVALUATION STUDY; INTERMETHOD COMPARISON; LIMIT OF DETECTION; LISTONELLA ANGUILLARUM; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MICROBIOLOGICAL EXAMINATION; MICROCHIP ANALYSIS; MICROFLUIDIC ANALYSIS; NOCARDIA; NOCARDIA SERIOLAE; NONHUMAN; NUCLEOTIDE SEQUENCE; PERFORMANCE MEASUREMENT SYSTEM; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS CONTROL; PROCESS DESIGN; PROCESS DEVELOPMENT; PSEUDOMONAS PUTIDA; RAPID TEST; REAL TIME FLUOROGENIC LOOP MEDIATED ISOTHERMAL AMPLIFICATION; REPRODUCIBILITY; SCREENING TEST; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS INIAE; SYSTEM ANALYSIS; VIBRIO; VIBRIO ALGINOLYTICUS; VIBRIO FLUVIALIS; VIBRIO HARVEYI; VIBRIO PARAHAEMOLYTICUS; VIBRIO ROTIFERIANUS; VIBRIO VULNIFICUS; ANIMAL; BACTERIA; CLASSIFICATION; DEVICES; ECONOMICS; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MICROFLUIDICS; NUCLEIC ACID AMPLIFICATION; PROCEDURES;

ANIMALS; AQUATIC ORGANISMS; BACTERIA; MICROFLUIDICS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SEAWATER;

EID: 84903690653     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2014.06.008     Document Type: Article

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