Real-time PCR-based microfluidic array chip for simultaneous detection of multiple waterborne pathogens

Sensors and Actuators, B: Chemical

Volumn 145, Issue 1, 2010, Pages 543-552

  Ramalingam, Naveen ^a   Rui, Zhang ^a   Liu, Hao Bing ^a   Dai, Chang Chun ^a   Kaushik, Rajni ^a   Ratnaharika, Bandi ^a   Gong, Hai Qing ^a  

^a NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

Author keywords

Capillary microfluidics; Microchip PCR; Microreactor array; Pathogen detection; Real time PCR

Indexed keywords

EVAPORATIVE LOSS; FLOW SCHEMES; GENOMIC DNA; LIQUID HANDLING; MICRO-FLUIDIC DEVICES; MICRO-REACTOR; MICROFLUIDIC ARRAY; MICROREACTOR DESIGN; OPERATION PROCESS; PATHOGEN DETECTION; REAL-TIME PCR; SIMULTANEOUS DETECTION; SINGLE-STEP; WATER-BORNE PATHOGENS;

BIOASSAY; BIOCHIPS; CHEMICAL REACTORS; DNA; FLUIDIC DEVICES; GENES; MICROFLUIDICS; MICROPROCESSOR CHIPS; THERMAL CYCLING;

PATHOGENS;

EID: 76949106026     PISSN: 09254005     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.snb.2009.11.025     Document Type: Article

References (24)

1. Water and Sanitation Related Diseases, World Health Organization (WHO) Fact Sheets (2004) 1-2.
2. Kong R.Y., Lee S.K., Law T.W., Law S.H., and Wu R.S. Rapid detection of six types of bacterial pathogens in marine waters by multiplex PCR. Water Res. 36 (2002) 2802-2812
3. Aridgides L.J., Doblin M.A., Berke T., Dobbs F.C., Matson D.O., and Drake L.A. Multiplex PCR allows simultaneous detection of pathogens in ships' ballast water. Mar. Pollut. Bull. 48 (2004) 1096-1101
4. Lee D.Y., Shannon K., and Beaudette L.A. Detection of bacterial pathogens in municipal wastewater using an oligonucleotide microarray and real-time quantitative PCR. J. Microbiol. Methods 65 (2006) 453-467
5. Yauk C.L., Berndt M.L., Williams A., and Douglas G.R. Comprehensive comparison of six microarray technologies. Nucleic Acids Res. 32 (2004) e124
6. Mitterer G., Huber M., Leidinger E., Kirisits C., Lubitz W., Mueller M.W., and Schmidt W.M. Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences. J. Clin. Microbiol. 42 (2004) 1048-1057
7. Strizhkov B.N., Drobyshev A.L., Mikhailovich V.M., and Mirzabekov A.D. PCR amplification on a microarray of gel-immobilized oligonucleotides: detection of bacterial toxin- and drug-resistant genes and their mutations. Biotechniques 29 (2000) 844-848, 850-852, 854, passim
8. Bing D.H., Boles C., Rehman F.N., Audeh M., Belmarsh M., and Kelley B. Bridge amplification: a solid phase PCR system for the amplification and detection of allelic differences in single copy genes. Genetic Identity Conference Proceedings, Seventh International Symposium on Human Identification (1996). http://www.promega.com/geneticidproc/ussymp7proc/0726.html http://www.promega.com/geneticidproc/ussymp7proc/0726.html
9. He J.W., and Jiang S. Quantification of enterococci and human adenoviruses in environmental samples by real-time PCR. Appl. Environ. Microbiol. 71 (2005) 2250-2255
10. Shannon K.E., Lee D.Y., Trevors J.T., and Beaudette L.A. Application of real-time quantitative PCR for the detection of selected bacterial pathogens during municipal wastewater treatment. Sci. Total Environ. 382 (2007) 121-129
11. Lee D.Y., Lauder H., Cruwys H., Falletta P., and Beaudette L.A. Development and application of an oligonucleotide microarray and real-time quantitative PCR for detection of wastewater bacterial pathogens. Sci. Total Environ. 398 (2008) 203-211
12. Stedtfeld R.D., Baushke S.W., Tourlousse D.M., Miller S.M., Stedtfeld T.M., Gulari E., Tiedje J.M., and Hashsham S.A. Development and experimental validation of a predictive threshold cycle equation for quantification of virulence and marker genes by high-throughput nanoliter-volume PCR on the OpenArray platform. Appl. Environ. Microbiol. 74 (2008) 3831-3838
13. McQuaig S.M., Scott T.M., Lukasik J.O., Paul J.H., and Harwood V.J. Quantification of human polyomaviruses JC Virus and BK Virus by TaqMan quantitative PCR and comparison to other water quality indicators in water and fecal samples. Appl. Environ. Microbiol. 75 (2009) 3379-3388
14. Nagai H., Murakami Y., Morita Y., Yokoyama K., and Tamiya E. Development of a microchamber array for picoliter PCR. Anal. Chem. 73 (2001) 1043-1047
15. Matsubara Y.K.K., Masaaki Kobayashi, Shouhei Yamanura, Yasutaka Morita, and Eiichi Tamiya. Microchamber array based DNA quantification and specific sequence detection from a single copy via PCR in nanoliter volumes. Biosens. Bioelectronics 20 (2005) 1482-1490
16. Morrison T., Hurley J., Garcia J., Yoder K., Katz A., Roberts D., Cho J., Kanigan T., Ilyin S.E., Horowitz D., Dixon J.M., and Brenan C.J. Nanoliter high throughput quantitative PCR. Nucleic Acids Res. 34 (2006) e123
17. Liu J., Hansen C., and Quake S.R. Solving the "world-to-chip" interface problem with a microfluidic matrix. Anal. Chem. 75 (2003) 4718-4723
18. Lagally E., Peter C., Simpson, and Mathies R.A. Monolithic integrated microfluidic DNA amplification and capillary electrophoresis analysis system. Sens. Actuators B 63 (2000) 138-146
19. Gong H., Ramalingam N., Chen L., Che J., Wang Q., Wang Y., Yang X., Yap P.H., and Neo C.H. Microfluidic handling of PCR solution and DNA amplification on a reaction chamber array biochip. Biomed. Microdevices 8 (2006) 167-176
20. Koh C.G., Tan W., Zhao M.Q., Ricco A.J., and Fan Z.H. Integrating polymerase chain reaction, valving, and electrophoresis in a plastic device for bacterial detection. Anal. Chem. 75 (2003) 4591-4598
21. Anderson R.C., Su X., Bogdan G.J., and Fenton J. A miniature integrated device for automated multistep genetic assays. Nucleic Acids Res. 28 (2000) E60
22. Yuen P.K., Kricka L.J., Fortina P., Panaro N.J., Sakazume T., and Wilding P. Microchip module for blood sample preparation and nucleic acid amplification reactions. Genome Res. 11 (2001) 405-412
23. Liu H.-B., Gong H.-Q., Ramalingam N., Jiang Y., Dai C.-C., and Hui K.M. Micro air bubble formation and its control during polymerase chain reaction (PCR) in polydimethylsiloxane (PDMS) microreactors. J. Micromech. Microeng. 17 (2007) 2055-2064
24. Wang F., Yang M., and Burns M.A. Microfabricated valveless devices for thermal bioreactions based on diffusion-limited evaporation. Lab Chip 8 (2008) 88-97