Development of a rapid assay to detect the dinoflagellate Amyloodinium ocellatum using loop-mediated isothermal amplification (LAMP)

Veterinary Parasitology

Volumn 196, Issue 3-4, 2013, Pages 265-271

  Picón Camacho, Sara M ^a   Thompson, William P ^a   Blaylock, Reginald B ^a   Lotz, Jeffrey M ^a  

^a UNIVERSITY OF SOUTHERN MISSISSIPPI   (United States)

Author keywords

Amyloodiniosis; Diagnostic test; Fish parasite; Velvet disease

Indexed keywords

RIBOSOME DNA;

AMYLOODINIUM OCELLATUM; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DINOFLAGELLATE; GENE AMPLIFICATION; GILL; INTERMETHOD COMPARISON; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TOMONT; TROPHONT; WATER ANALYSIS;

AMYLOODINIUM OCELLATUM; DINOPHYCEAE; PISCES;

AMYLOODINIOSIS; DIAGNOSTIC TEST; FISH PARASITE; VELVET DISEASE;

ANIMALS; BASE SEQUENCE; DINOFLAGELLIDA; DNA; FISH DISEASES; FISHES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SENSITIVITY AND SPECIFICITY; TIME FACTORS; WATER;

EID: 84881556266     PISSN: 03044017     EISSN: 18732550     Source Type: Journal    
DOI: 10.1016/j.vetpar.2013.04.010     Document Type: Article

References (36)

1. Adams A., Thompson K.D. Development of diagnostics for aquaculture: challenges and opportunities. Aquacult. Res. 2011, 42:93-102.
2. Auderman C., Reece K.S., Burreson E.M. Real-Time PCR for detection and quantification of the protistan parasite Perkinsus marinus in environmental waters. Appl. Environ. Microbiol. 2004, 70:6611-6618.
3. Blaylock R.B., Whelan D.S. Fish health management for offshore aquaculture in the Gulf of Mexico. Efforts to Develop a Responsible Offshore Aquaculture Industry in the Gulf of Mexico: A Compendium of Offshore Aquaculture Consortium Research 2004, 129-161. Mississippi-Alabama Sea Grant Consortium, Ocean Springs. C.J. Bridger (Ed.).
4. Bower C.E. Update on Amyloodinium. SeaScope 1987, 4:1-4.
5. Brown E.M. On Amyloodinium ocellatum Brown, a parasitic dinoflagellate causing epidemic disease in marine fish. Proc. Zool. Soc. Lond. 1934, 3:583-607.
6. Fu S., Qu G., Guo S., Ma L., Zhang N., Zhang S., Gao S., Shen Z. Applications of Loop-mediated isothermal DNA-amplification. Appl. Biochem. Biotechnol. 2011, 163:845-850.
7. Hall T.A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 1999, 41:95-98.
8. Kuperman B.I., Matey V.E. Massive infestation by Amyloodinium ocellatum (Dinoflagellida) of fish in a highly saline lake, Salton Sea, CA, USA. Dis. Aquat. Org. 1999, 39:65-73.
9. Lawler A.R. The parasitic dinoflagellate Amyloodinium ocellatum in marine aquaria. Drum Croaker 1977, 17:17-20.
10. Lawler A.R. Studies on Amyloodinium ocellatum (Dinoflagellata) in Mississippi Sound: natural and experimental hosts. Gulf Res. Rep. 1980, 6:403-413.
11. Levy M.G., Poore M.F., Colorni A., Noga E.J., Vandersea M.W., Litaker R.W. A highly specific PCR assay for detecting the fish ectoparasite Amyloodinium ocellatum. Dis. Aquat. Organ. 2007, 73:219-226.
12. Lewis D.H., Wenxing W., Ayers A., Arnold C.R. Preliminary studies on the use of chloroquine as systemic chemotherapeutic agent for amyloodinosis in red drum (Sciaenops ocellatus). Contrib. Marine Sci. 1988, 30. 193-189.
13. Lom J., Lawler A.R. A structural study of the mode of attachment in dinoflagellates invading gills of Cyprinodontidae. Protistologica 1973, 9:293-309.
14. Masson I., Blaylock R.B., Lotz J.M. Susceptibility and tolerance of spotted seatrout, Cynoscion nebulosus, and red snapper, Lutjanus campechanus, to experimental infections with Amyloodinium ocellatum. J. Parasitol. 2011, 97:577-585.
15. Montgomery-Brock, D.R., Sylvester, J.Y., Tamaru, C.S., Brock, J.A., 2000. Hydrogen peroxide treatment for Amyloodinium sp. on mullet (Mugil cephalus) fry. Aqua Tips, Regional Notes, Center for Tropical and Subtropical Aquaculture 11, 4-6.
16. Montgomery-Brock D., Brock J.A., Tamaru C.S. The application of hydrogen peroxide as a treatment for the ectoparasite Amyloodinium ocellatum (Brown 1931) on the Pacific threadfin Polydactylus sexfilis. J. World Aquacult. Soc. 2001, 32:250-254.
17. Mori Y., Nagamine K., Tomita N., Notomi T. Detection of Loop-Mediated Isothermal Amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 2001, 289:150-154.
18. Mori Y., Notomi T. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J. Infect. Chemother. 2009, 15:62-69.
19. Nagamine K., Hase T., Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol. Cell. Probes 2002, 16:223-229.
20. Nagamine K., Watanabe K., Ohtsuka K., Hase T., Notomi T. Loop-mediated isothermal amplification reaction using a non denaturated template. Clin. Chem. 2001, 47:1742-1743.
21. Nigrelli R.F. The morphology, cytology and life-history of Oodinium ocellatum Brown, a dinoflagellate parasitic of marine fishes. Zoologica 1936, 21:129-164.
22. Noga E.J., Levy M.G. Phylum dinoflagellata. Fish Diseases and Disorders, Volume 1: Protozoan and Metazoan Infections 2006, 16-45. CABI International, Oxford. 2nd edition. P.T.K. Woo (Ed.).
23. Noga E.J. Fish Disease: Diagnosis and Treatment 2010, Wiley-Blackwell, Ames, 367 pp.
24. Noga E.J. Amyloodinium ocellatum. Fish Parasites: Pathobiology and Protection 2012, 383. CAB International, Croydon. P.T.K. Woo, K. Buchmann (Eds.).
25. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000, 28:e63.
26. Oestmann D.J., Lewis D.H., Zettler B.A. Clearance of Amyloodinium ocellatum dinospores by Artemia salina. J. Aquat. Anim. Health 1995, 7:257-261.
27. Paperna I. Reproduction cycle and tolerance to temperature and salinity of Amyloodinium ocellatum (Brown, 1931) (Dinoflagellida). Ann. Parasitol. Hum. Comp. 1984, 59:7-30.
28. Paperna I. Chemical control of Amyloodinium ocellatum (Brown 1931) (Dinoflagellida) infections in vitro tests and treatment trials with infected fishes. Aquaculture 1984, 38:l-l18.
29. Pereira J.C., Abrantes I., Martins I., Barata J., Frias P., Pereira I. Ecological and morphological features of Amyloodinium ocellatum ocurrences in cultivated gilthead seabream Sparus aurata L.: a case study. Aquaculture 2011, 310:289-297.
30. Pottinger T.G. The stress response of in fish - mechanisms, effects and measurement. Fish Welfare 2007, 78-92. Wiley-Blackwell, Oxford. E.J. Fish Branson (Ed.).
31. Sakai M., Baxa D.V., Kurobe T., Kono T., Shivappa R.B., Hedrick R.P. Detection of Nucleospora salmonis in cutthroat trout (Oncorhynchus clarki) and rainbow trout (Oncorhynchus mykis) by loop-mediated isothermal amplification. Aquaculture 2009, 288:27-31.
32. Savan R., Kono T., Itami T., Sakai M. Loop-mediated isothermal amplification: an emerging technology for detection of fish and shellfish pathogens. J. Fish Dis. 2005, 28:573-581.
33. Soliman H., El-Matbouli M. An expensive and rapid diagnostic method of Koi Herpesvirus (KHV) infection by loop-mediated isothermal amplification. Virol. J. 2005, 2:83.
34. Soliman H., El-Matbouli M. Loop mediated isothermal amplification combined with nucleic acid lateral flow strip for diagnosis of cyprinid herpes virus-3. Mol. Cell. Probes 2010, 24:38-43.
35. Tanner N.A., Zhang Y., Evans T.C. Simultaneous multiple target detection in real-time loop-mediated isothermal amplification. Biotechniques 2012, 53:81-89.
36. Tourlousse D.M., Ahmad F., Stedtfeld R.D., Seyrig G., Tiedje J.M., Hashsham S.A. A polymer microfluidic chip for quantitative detection of multiple water- and foodborne pathogens using real-time fluorogenic loop-mediated isothermal amplification. Biomed. Microdevices 2012, 14:769-778.