Isolation and characterization of a thermotolerant ene reductase from Geobacillus sp. 30 and its heterologous expression in Rhodococcus opacus

Applied Microbiology and Biotechnology

Volumn 98, Issue 13, 2014, Pages 5925-5935

  Tsuji, Naoto ^a   Honda, Kohsuke ^a   Wada, Mayumi ^a   Okano, Kenji ^a   Ohtake, Hisao ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Ene reductase; Geobacillus sp; Lipophilic bacteria; Rhodococcus opacus; Thermophilic enzyme

Indexed keywords

CELLS; CYTOLOGY; GENE ENCODING; GENE EXPRESSION; MIXTURES;

ENANTIOSELECTIVE REDUCTION; ENE REDUCTASE; GEOBACILLUS SP; HETEROLOGOUS EXPRESSION; HIGH REACTION TEMPERATURES; ISOLATION AND CHARACTERIZATION; RHODOCOCCUS OPACUS; THERMOPHILIC ENZYMES;

ENZYMES;

CYCLOHEXANOL; ENE REDUCTASE; OXIDOREDUCTASE; UNCLASSIFIED DRUG;

BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; RECOMBINATION; TEMPERATURE TOLERANCE; THERMOPHILY;

ARTICLE; BIOTRANSFORMATION; CATALYST; CHROMATOGRAPHY; COLUMN CHROMATOGRAPHY; DEHYDROGENATION; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME STABILITY; GENE EXPRESSION; GENE IDENTIFICATION; GEOBACILLUS; HEAT TOLERANCE; HETEROLOGOUS EXPRESSION; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; REGENERATION; RHODOCOCCUS OPACUS; THERMOSTABILITY; THERMUS THERMOPHILUS;

GEOBACILLUS; RHODOCOCCUS OPACUS; THERMUS THERMOPHILUS;

EID: 84903575609     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5668-9     Document Type: Article

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