Directed evolution of thermotolerant malic enzyme for improved malate production

Journal of Bioscience and Bioengineering

Volumn 117, Issue 2, 2014, Pages 147-152

  Morimoto, Yumi ^a   Honda, Kohsuke ^a,b   Ye, Xiaoting ^a   Okano, Kenji ^a   Ohtake, Hisao ^a  

^a OSAKA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Cofactor preference; Directed evolution; HCO3 fixation; Malic enzyme; Reaction specificity

Indexed keywords

CATALYTIC MECHANISMS; COFACTOR PREFERENCES; DIRECTED EVOLUTION; MALIC ENZYMES; REACTION SPECIFICITY; SINGLE-POINT MUTATION; TRIPLE-MUTANT ENZYME; WILD-TYPE ENZYMES;

CARBON DIOXIDE; CARBOXYLATION; CATALYSIS;

ENZYMES;

MALATE DEHYDROGENASE (DECARBOXYLATING); MALIC ACID; NUCLEOTIDE; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AMINO ACID SUBSTITUTION; ARTICLE; CARBOXYLATION; CATALYSIS; CHEMICAL REACTION KINETICS; COLORIMETRY; ENZYME ASSAY; ENZYME ISOLATION; ESCHERICHIA COLI; HEAT TOLERANCE; HIGH THROUGHPUT SCREENING; NONHUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION; SCREENING; SEQUENCE ANALYSIS; THERMOCOCCUS KODAKARENSIS;

THERMOCOCCUS KODAKARENSIS;

COFACTOR PREFERENCE; DIRECTED EVOLUTION; FIXATION; MALIC ENZYME; REACTION SPECIFICITY;

BIOCATALYSIS; DIRECTED MOLECULAR EVOLUTION; ENZYME ASSAYS; GENE LIBRARY; KINETICS; MALATE DEHYDROGENASE; MALATES; NAD; NADP; OXIDATION-REDUCTION; POINT MUTATION; PROTEIN ENGINEERING; PYRUVIC ACID; SUBSTRATE SPECIFICITY; THERMOCOCCUS;

EID: 84892370944     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2013.07.005     Document Type: Article

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