Systematic screening of Escherichia coli single-gene knockout mutants for improving recombinant whole-cell biocatalysts

Applied Microbiology and Biotechnology

Volumn 87, Issue 2, 2010, Pages 647-655

  Zhou, Ying ^a   Minami, Takeshi ^a   Honda, Kohsuke ^a   Omasa, Takeshi ^a   Ohtake, Hisao ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Cytochrome P450 monooxygenase; Keio collection; Systematic screening; Whole cell biocatalysis

Indexed keywords

7-ETHOXYCOUMARIN; 96-WELL PLATE; COMPLEMENTATION; CONTROL STRAIN; CYTOCHROME P450; DELETION MUTANTS; E. COLI; HIGH-THROUGHPUT SCREENING; KEIO COLLECTION; MONOOXYGENASES; POLAR EFFECT; SINGLE-GENE KNOCKOUT; TEST TUBE; WHOLE CELL BIOCATALYSTS; WHOLE-CELL BIOCATALYSIS;

ENZYMES; ESCHERICHIA COLI; GENES;

ENZYME ACTIVITY;

7 ETHOXYCOUMARIN; CYP154A1; CYTOCHROME; UNCLASSIFIED DRUG; 7-ETHOXYCOUMARIN; COUMARIN DERIVATIVE; CYTOCHROME P450; ENZYME; ESCHERICHIA COLI PROTEIN;

CATALYSIS; COLIFORM BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ENGINEERING; GENOME; MUTATION; RECOMBINATION;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL GENOME; BIOCATALYSIS; BIOCATALYST; CONTROLLED STUDY; CPXA GENE; DELETION MUTANT; ENZYME ACTIVITY; ESCHERICHIA COLI; GCVR GENE; GENOME ANALYSIS; GLNL GENE; NONHUMAN; SPONTANEOUS MUTATION; UK GENE; WHOLE CELL; ENZYMOLOGY; GENE INACTIVATION; GENETICS; METABOLISM; MUTATION;

COUMARINS; CYTOCHROME P-450 ENZYME SYSTEM; ENZYMES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE KNOCKOUT TECHNIQUES; MUTATION;

ESCHERICHIA COLI;

EID: 77953083400     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2505-7     Document Type: Article

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