Effect of autophagy inhibition on cell viability and cell cycle progression in MDA-MB-231 human breast cancer cells

Molecular Medicine Reports

Volumn 10, Issue 2, 2014, Pages 625-630

  Liu, Qiujun ^a,b   Shi, Xinli ^c   Zhou, Xianyao ^a   Wang, D A ^a   Wang, L I ^b   Li, Changlong ^a  

^a SICHUAN UNIVERSITY   (China)

^b SOUTHWEST MEDICAL UNIVERSITY   (China)

^c HEBEI UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

3 methyladenine; Atg7 deficiency; Autophagy inhibition; Breast cancer; Cell cycle; MDA MB 231 cells

Indexed keywords

GLUCOSE; SMALL INTERFERING RNA; 3-METHYLADENINE; ADENINE; ATG7 PROTEIN, HUMAN; UBIQUITIN PROTEIN LIGASE;

APOPTOSIS; ARTICLE; ATG7 GENE; AUTOPHAGY; BREAST CANCER; CANCER CELL; CANCER INHIBITION; CELL CYCLE PROGRESSION; CELL CYCLE S PHASE; CELL VIABILITY; CONTROLLED STUDY; G1 PHASE CELL CYCLE CHECKPOINT; GENE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MTT ASSAY; ANALOGS AND DERIVATIVES; ANTAGONISTS AND INHIBITORS; BREAST TUMOR; CELL CYCLE CHECKPOINT; CELL SURVIVAL; DRUG EFFECTS; FEMALE; GENETICS; METABOLISM; PATHOLOGY; RNA INTERFERENCE; TUMOR CELL LINE;

ADENINE; APOPTOSIS; AUTOPHAGY; BREAST NEOPLASMS; CELL CYCLE CHECKPOINTS; CELL LINE, TUMOR; CELL SURVIVAL; FEMALE; HUMANS; RNA INTERFERENCE; RNA, SMALL INTERFERING; UBIQUITIN-ACTIVATING ENZYMES;

EID: 84903267348     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2014.2296     Document Type: Article

References (23)

1. Ahn JH and Lee M: Autophagy-dependent survival of mutant B-raf melanoma cells selected for resistance to apoptosis induced by inhibitors against oncogenic B-raf. Biomol Ther (Seoul) 21: 114-120, 2013.
2. Guo JY, Karsli-Uzunbas G, Mathew R, Aisner SC, Kamphorst JJ, Strohecker AM, Chen G, Price S, Lu W, Teng X, et al: Autophagy suppresses progression of K-ras-induced lung tumors to oncocytomas and maintains lipid homeostasis. Genes Dev 27: 1447-1461, 2013.
3. Zhu L, Du H, Shi M, Chen Z and Hang J: ATG7 deficiency promote apoptotic death induced by Cisplatin in human esophageal squamous cell carcinoma cells. Bull Cancer 100: 15-21, 2013.
4. Shen J, Zheng H, Ruan J, Fang W, Li A, Tian G, Niu X, Luo S and Zhao P: Autophagy inhibition induces enhanced proapoptotic effects of ZD6474 in glioblastoma. Br J Cancer 109: 164-171, 2013.
5. Tong Y, You L, Liu H, Li L, Meng H, Qian Q and Qian W: Potent antitumor activity of oncolytic adenovirus expressing Beclin-1 via induction of autophagic cell death in leukemia. Oncotarget 4: 860-874, 2013.
6. Basit F, Cristofanon S and Fulda S: Obatoclax (GX15-070) triggers necroptosis by promoting the assembly of the necrosome on autophagosomal membranes. Cell Death Differ 20: 1161-1173, 2013.
7. Yue W, Hamaï A, Tonelli G, Bauvy C, Nicolas V, Tharinger H, Codogno P and Mehrpour M: Inhibition of the autophagic flux by salinomycin in breast cancer stem-like/progenitor cells interferes with their maintenance. Autophagy 9: 714-729, 2013.
8. Kumar S, Kumar A, Pathania AS, Guru SK, Jada S, Sharma PR, Bhushan S, Saxena AK, Kumar HM and Malik F: Tiron and trolox potentiate the autophagic cell death induced by magnolol analog Ery5 by activation of Bax in HL-60 cells. Apoptosis 18: 605-617, 2013.
9. Park JH, Lee JE, Lee SJ, Park SJ, Park KH, Jeong M and Koh HC: Potential autophagy enhancers protect against fipronil-induced apoptosis in SH-SY5Y cells. Toxicol Lett 223: 25-34, 2013.
10. Qu W, Xiao J, Zhang H, Chen Q, Wang Z, Shi H, Gong L, Chen J, Liu Y, Cao R and Lv J: B19, a novel monocarbonyl analogue of curcumin, induces human ovarian cancer cell apoptosis via activation of endoplasmic reticulum stress and the autophagy signaling pathway. Int J Biol Sci 9: 766-777, 2013.
11. Polewska J, Skwarska A, Augustin E and Konopa J: DNA-damaging imidazoacridinone C-1311 induces autophagy followed by irreversible growth arrest and senescence in human lung cancer cells. J Pharmacol Exp Ther 346: 393-405, 2013.
12. Wei YM, Li X, Xu M, Abais JM, Chen Y, Riebling CR, Boini KM, Li PL and Zhang Y: Enhancement of autophagy by simvastatin through inhibition of Rac1-mTOR signaling pathway in coronary arterial myocytes. Cell Physiol Biochem 31: 925-937, 2013.
13. Ye L, Zhao X, Lu J, Qian G, Zheng JC and Ge S: Knockdown of TIGAR by RNA interference induces apoptosis and autophagy in HepG2 hepatocellular carcinoma cells. Biochem Biophys Res Commun 437: 300-306, 2013.
14. Sheng Y, Sun B, Guo WT, Zhang YH, Liu X, Xing Y and Dong DL: 3-Methyladenine induces cell death and its interaction with chemotherapeutic drugs is independent of autophagy. Biochem Biophys Res Commun 432: 5-9, 2013.
15. Lei FR, Li XQ, Liu H, Zhu RD, Meng QY and Rong JJ: Rapamycin and 3-methyladenine regulate apoptosis and autophagy in bone-derived endothelial progenitor cells. Chin Med J (Engl) 125: 4076-4082, 2012.
16. Lin YC, Kuo HC, Wang JS and Lin WW: Regulation of inflammatory response by 3-methyladenine involves the coordinative actions on Akt and glycogen synthase kinase 3β rather than autophagy. J Immunol 189: 4154-4164, 2012.
17. Chang TK, Shravage BV, Hayes SD, Powers CM, Simin RT, Wade Harper J and Baehrecke EH: Uba1 functions in Atg7- and Atg3-independent autophagy. Nat Cell Biol 15: 1067-1078, 2013.
18. Qin Z, Xue J, He Y, Ma H, Jin G, Chen J, Hu Z, Liu X and Shen H: Potentially functional polymorphisms in ATG10 are associated with risk of breast cancer in a Chinese population. Gene 527: 491-495, 2013.
19. Balmer D, Emery M, Andreux P, Auwerx J, Ginet V, Puyal J, Schorderet DF and Roduit R: Autophagy defect is associated with low glucose-induced apoptosis in 661W photoreceptor cells. PLoS One 8: e74162, 2013.
20. Ramírez-Peinado S, León-Annicchiarico CL, Galindo-Moreno J, Iurlaro R, Caro-Maldonado A, Prehn JH, Ryan KM and Muñoz-Pinedo C: Glucose-starved cells do not engage in prosurvival autophagy. J Biol Chem 288: 30387-30398, 2013.
21. Pliyev BK and Menshikov M: Differential effects of the autophagy inhibitors 3-methyladenine and chloroquine on spontaneous and TNF-α-induced neutrophil apoptosis. Apoptosis 17: 1050-1065, 2012.
22. Tseng HC, Liu WS, Tyan YS, Chiang HC, Kuo WH and Chou FP: Sensitizing effect of 3-methyladenine on radiation-induced cytotoxicity in radio-resistant HepG2 cells in vitro and in tumor xenografts. Chem Biol Interact 192: 201-208, 2011.
23. Wu YT, Tan HL, Shui G, Bauvy C, Huang Q, Wenk MR, Ong CN, Codogno P and Shen HM: Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. J Biol Chem 285: 10850-10861, 2010.