Engineering pyruvate decarboxylase-mediated ethanol production in the thermophilic host Geobacillus thermoglucosidasius

Applied Microbiology and Biotechnology

Volumn 98, Issue 3, 2014, Pages 1247-1259

  Van Zyl, L J ^a   Taylor, M P ^a,b   Eley, K ^b   Tuffin, M ^a   Cowan, D A ^a,c  

^a UNIVERSITY OF THE WESTERN CAPE   (South Africa)

^b TMO Renewables Ltd   (United Kingdom)

^c UNIVERSITY OF PRETORIA   (South Africa)

Author keywords

Bioethanol; Gluconobacter spp.; Pyruvate decarboxylase; Thermophilic expression

Indexed keywords

BIOETHANOL; ENZYME ACTIVITY;

CATALYTIC EFFICIENCIES; GEOBACILLUS THERMOGLUCOSIDASIUS; GLUCONOBACTER; GLUCONOBACTER OXYDANS; HETEROLOGOUS EXPRESSION; KINETIC CHARACTERIZATION; PYRUVATE DECARBOXYLASE; THERMOPHILIC EXPRESSION;

ETHANOL;

ALCOHOL; MESSENGER RNA; PYRUVATE DECARBOXYLASE; CODON; GLUCOSE; PYRUVIC ACID;

BACTERIUM; BIOENGINEERING; CATALYSIS; ENZYME ACTIVITY; ETHANOL; GENE EXPRESSION; GLUCOSE; GROWTH RATE; INDUSTRIAL PRODUCTION; MUTATION; PH; TEMPERATURE EFFECT;

ALCOHOL PRODUCTION; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; CODON USAGE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; GEOBACILLUS; GEOBACILLUS THERMOGLUCOSIDASIUS; GLUCONOBACTER OXYDANS; HETEROLOGOUS EXPRESSION; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PROTEIN EXPRESSION; STEADY STATE; TEMPERATURE; THERMOPHILIC BACTERIUM; WESTERN BLOTTING; CHEMISTRY; CODON; ENZYMOLOGY; FERMENTATION; GENETICS; KINETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

GEOBACILLUS; GEOBACILLUS THERMOGLUCOSIDASIUS; GLUCONOBACTER; GLUCONOBACTER OXYDANS;

CODON; ENZYME STABILITY; ETHANOL; FERMENTATION; GEOBACILLUS; GLUCONOBACTER OXYDANS; GLUCOSE; HYDROGEN-ION CONCENTRATION; KINETICS; METABOLIC ENGINEERING; PYRUVATE DECARBOXYLASE; PYRUVIC ACID; TEMPERATURE;

EID: 84898884130     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5380-1     Document Type: Article

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