Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida

Protein Engineering, Design and Selection

Volumn 18, Issue 7, 2005, Pages 345-357

  Siegert, Petra ^a,c   McLeish, Michael J ^b   Baumann, Martin ^a,d   Iding, Hans ^a,e   Kneen, Malea M ^b   Kenyon, George L ^b   Pohl, Martina ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b UNIVERSITY OF MICHIGAN   (United States)

^c HENKEL KGAA   (Germany)

^d ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^e ROCHE INNOVATION CENTER MUNICH   (Germany)

Author keywords

Carboligation; Decarboxylation; Substrate range; Thiamine diphosphate

Indexed keywords

CATALYSIS; ORGANIC ACIDS; X RAYS;

CARBOLIGATION; SUBSTRATE RANGE; X-RAY STRUCTURES;

ENZYMES;

ACETALDEHYDE; ACETOIN; ALIPHATIC COMPOUND; BENZOIN; HEXANOIC ACID; METHANOL; OXOACID; PHENYLGLYOXYLIC ACID; PROPIOPHENONE DERIVATIVE; PYRUVATE DECARBOXYLASE; PYRUVIC ACID; THIAMINE; VALERIC ACID DERIVATIVE;

ARTICLE; CATALYSIS; CHIMERA; DECARBOXYLATION; ENZYME MECHANISM; ENZYME SPECIFICITY; NONHUMAN; PARAMETER; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA; REACTION ANALYSIS; SITE DIRECTED MUTAGENESIS; SPECTROSCOPY; STEREOCHEMISTRY; X RAY ANALYSIS; ZYMOMONAS MOBILIS;

AMINO ACID SEQUENCE; CARBOXY-LYASES; MUTAGENESIS, SITE-DIRECTED; PSEUDOMONAS PUTIDA; PYRUVATE DECARBOXYLASE; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; THIAMINE PYROPHOSPHATE; ZYMOMONAS;

PSEUDOMONAS PUTIDA; ZYMOMONAS MOBILIS;

EID: 25844473249     PISSN: 17410126     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzi035     Document Type: Article

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