Molecular characterization of KU70 and KU80 homologues and exploitation of a KU70-deficient mutant for improving gene deletion frequency in Rhodosporidium toruloides

BMC Microbiology

Volumn 14, Issue 1, 2014, Pages

  Koh, Chong Mei John ^a   Liu, Yanbin ^a   Moehninsi, ^a   Du, Minge ^a   Ji, Lianghui ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Agrobacterium tumefaciens mediated transformation (ATMT); Homologous recombination; Nonhomologous end joining (NHEJ); Oleaginous yeast; Rhodosporidium toruloides

Indexed keywords

CRE RECOMBINASE; HYGROMYCIN; MUTAGENIC AGENT; FUNGAL DNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AGROBACTERIUM TUMEFACIENS; ARTICLE; CELL GROWTH; CELL STRUCTURE; CONTROLLED STUDY; DELETION MUTANT; DNA BASE COMPOSITION; DNA FLANKING REGION; FUNGAL GENE; GENE DELETION; GENE FREQUENCY; GENE ISOLATION; GENE TARGETING; GENOME; HOMOLOGOUS RECOMBINATION; INTRON; KU70 GENE; KU80 GENE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; RHODOSPORIDIUM TORULOIDES; RHODOTORULA GLUTINIS; SENSITIVITY ANALYSIS; SEQUENCE HOMOLOGY; SOUTHERN BLOTTING; ULTRAVIOLET RADIATION; YARROWIA LIPOLYTICA; BASIDIOMYCETES; CHEMISTRY; DNA SEQUENCE; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; MICROBIAL GENETICS; MOLECULAR GENETICS; PROCEDURES;

AGROBACTERIUM TUMEFACIENS; RHODOSPORIDIUM TORULOIDES; YARROWIA LIPOLYTICA;

AGROBACTERIUM TUMEFACIENS; BASIDIOMYCOTA; DNA, FUNGAL; GENE DELETION; GENE TARGETING; GENETIC VECTORS; GENETICS, MICROBIAL; MOLECULAR SEQUENCE DATA; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, GENETIC;

EID: 84898548564     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-14-50     Document Type: Article

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