Characterization of glyceraldehyde-3-phosphate dehydrogenase gene RtGPD1 and development of genetic transformation method by dominant selection in oleaginous yeast Rhodosporidium toruloides

Applied Microbiology and Biotechnology

Volumn 97, Issue 2, 2013, Pages 719-729

  Liu, Yanbin ^a   Koh, Chong Mei John ^a   Sun, Longhua ^a   Hlaing, Mya Myintzu ^a   Du, Minge ^a   Peng, Ni ^a   Ji, Lianghui ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Agrobacterium tumefaciens mediated transformation; Glyceraldehyde 3 phosphate dehydrogenase; Microbial oil; Oleaginous yeast; Rhodosporidium toruloides

Indexed keywords

AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; MICROBIAL OIL; OLEAGINOUS YEASTS; RHODOSPORIDIUM TORULOIDES;

AMINO ACIDS; NUCLEIC ACIDS; OPTIMIZATION; YEAST;

GENE EXPRESSION;

AMINO ACID; CYTOSINE; ENHANCED GREEN FLUORESCENT PROTEIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYGROMYCIN;

BIOACCUMULATION; BIOASSAY; BIOFUEL; BIOMASS; DNA; ENZYME ACTIVITY; FERMENTATION; GENE; GENETIC ANALYSIS; LIPID; MICROBIAL COMMUNITY; YEAST;

ANALYSIS; ARTICLE; CARBON SOURCE; CODON; EXON; FLUORESCENCE MICROSCOPY; GENETIC TRANSFORMATION; INVERSE POLYMERASE CHAIN REACTION; NONHUMAN; NORTHERN BLOTTING; OPEN READING FRAME; OSMOTIC STRESS; PROTEIN ISOLATION; RHODOSPORIDIUM TORULOIDES; SHOCK; SOUTHERN BLOTTING; TEMPERATURE; USTILAGO MAYDIS;

AGROBACTERIUM TUMEFACIENS; BASIDIOMYCOTA; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; TRANSFORMATION, GENETIC;

AGROBACTERIUM TUMEFACIENS; BASIDIOMYCOTA; RHODOSPORIDIUM TORULOIDES; USTILAGO MAYDIS;

EID: 84873992985     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4223-9     Document Type: Article

References (37)

1. Brachmann A, König J, Julius C, Feldbrügge M (2004) A reverse genetic approach for generating gene replacement mutants in Ustilago maydis. Mol Genet Genomics 272:216-226
2. Brefort T, Doehlemann G, Mendoza-Mendoza A, Reissmann S, Djamei A, Kahmann R (2009) Ustilago maydis as a pathogen. Annu Rev Phytopathol 47:423-445
3. Burns C, Gregory KE, Kirby M, Cheung MK, Riquelme M, Elliott TJ, Challen MP, Bailey A, Foster GD (2005) Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns. Fungal Genet Biol 42:191-199
4. Cangelosi GA, Best EA, Martinetti G, Nester EW (1991) Genetic analysis of Agrobacterium. Methods Enzymol 204:384-397
5. Chan CS, Guo L, Shih MC (2001) Promoter analysis of the nuclear gene encoding the chloroplast glyceraldehyde-3-phosphate dehydrogenase B subunit of Arabidopsis thaliana. Plant Mol Biol 46:131-141
6. Halaouli S, Record E, Casalot L, Hamdi M, Sigoillot JC, Asther M, Lomascolo A (2006) Cloning and characterization of a tyrosinase gene from the white-rot fungus Pycnoporus sanguineus, and overproduction of the recombinant protein in Aspergillus niger. Appl Microbiol Biotechnol 70:580-589
7. Harmsen MC, Schuren FH, Moukha SM, van Zuilen CM, Punt PJ, Wessels JG (1992) Sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase genes from the basidiomycetes Schizophyllum commune, Phanerochaete chrysosporium and Agaricus bisporus. Curr Genet 22:447-454
8. Harris JI, Waters M (1976) Glyceraldehyde 3 phosphate dehydrogenase. In: Boyer PD (ed) The enzymes. Academic Press, New York, pp 1-49
9. Holland MJ, Holland JP (1978) Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase. Biochemistry 17:4900-4907
10. Hooykaas PJJ, Roobol C, Schilperoort RA (1979) Regulation of the transfer of Ti-plasmids of Agrobacterium tumefaciens. J Gen Microbiol 110:99-109
11. Ianiri G, Wright SA, Castoria R, Idnurm A (2011) Development of resources for the analysis of gene function in Pucciniomycotina red yeasts. Fungal Genet Biol 48:685-695
12. Ji L, Jiang ZD, Liu Y, Koh CM, Zhang LH (2010) A simplified and efficient method for transformation and gene tagging of Ustilago maydis using frozen cells. Fungal Genet Biol 47:279-287
13. Kilaru S, Kües U (2005) Comparison of gpd genes and their protein products in basidiomycetes. Fungal Genet Newsl 52:18-23
14. Kilaru S, Hoegger P, Majcherczyk A, Burns C, Shishido K, Bailey A, Foster G, Kües U (2006) Expression of laccase gene lcc1 in Coprinopsis cinerea under control of various basidiomycetous promoters. Appl Microbiol Biotechnol 71:200-210
15. Lawrence GJ, Dodds PN, Ellis JG (2010) Transformation of the flax rust fungus, Melampsora lini: selection via silencing of an avirulence gene. Plant J 61:364-369
16. Lazo GR, Stein PA, Ludwig RA (1991) A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Nat Biotechnol 9:963-967
17. Levitt N, Briggs D, Gil A, Proudfoot NJ (1989) Definition of an efficient synthetic poly(A) site. Genes Dev 3:1019-1025
18. Liu H, Zhao X, Wang F, Li Y, Jiang X, Ye M, Zhao ZK, Zou H (2009) Comparative proteomic analysis of Rhodosporidium toruloides during lipid accumulation. Yeast 26:553-566
19. Liu Y, Koh CM, Sun L, Ji L (2011) Tartronate semialdehyde reductase defines a novel rate-limiting step in assimilation and bioconversion of glycerol in Ustilago maydis. PLoS One 6:e16438
20. Loubradou G, Brachmann A, Feldbrügge M, Kahmann R (2001) A homologue of the transcriptional repressor Ssn6p antagonizes cAMP signalling in Ustilago maydis. Mol Microbiol 40:719-730
21. Ma B, Mayfield MB, Gold MH (2001) The green fluorescent protein gene functions as a reporter of gene expression in Phanerochaete chrysosporium. Appl Environ Microbiol 67:948-955
22. Nitta Y, Miyazaki Y, Nakamura M, Iimura Y, Shishido K, Kajita S, Morohoshi N (2004) Molecular cloning of the promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene that contributes to the construction of a new transformation system in Coriolus versicolor. Mycoscience 45:131-136
23. Ochman H, Gerber AS, Hartl DL (1988) Genetic applications of an inverse polymerase chain reaction. Genetics 120:621-623
24. Punt PJ, Dingemanse MA, Kuyvenhoven A, Soede RD, Pouwels PH, van den Hondel CA (1990) Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase. Gene 93:101-109
25. Redkar RJ, Herzog RW, Singh NK (1998) Transcriptional activation of the Aspergillus nidulans gpdA promoter by osmotic signals. Appl Environ Microbiol 64:2229-2231
26. Sampaio JP, Gadanho M, Bauer R, Weiß M (2003) Taxonomic studies in the Microbotryomycetidae: Leucosporidium golubevii sp. nov., Leucosporidiella gen. nov. and the new orders Leucosporidiales and Sporidiobolales. Mycol Prog 2:53-68
27. Schuren FH, Wessels JG (1994) Highly-efficient transformation of the homobasidiomycete Schizophyllum commune to phleomycin resistance. Curr Genet 26:179-183
28. Smith TL, Leong SA (1990) Isolation and characterization of a Ustilago maydis glyceraldehyde-3-phosphate dehydrogenase-encoding gene. Gene 93:111-117
29. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731-2739
30. Tristan C, Shahani N, Sedlak TW, Sawa A (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal 23:317-323
31. Tsuchiya K, Tajima H, Yamada M, Takahashi H, Kuwae T, Sunaga K, Katsube N, Ishitani R (2004) Disclosure of a pro-apoptotic glyceraldehyde-3-phosphate dehydrogenase promoter: anti-dementia drugs depress its activation in apoptosis. Life Sci 74:3245-3258
32. Tully M, Gilbert HJ (1985) Transformation of Rhodosporidium toruloides. Gene 36:235-240
33. Turcotte G, Kosaric N (1988) Biosynthesis of lipids by Rhodosporidium toruloides ATCC 10788. J Biotechnol 8:221-237
34. Vassileva A, Chugh DA, Swaminathan S, Khanna N (2001) Expression of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris using the GAP promoter. J Biotechnol 88:21-35
35. Webb CA, Szabo LJ, Bakkeren G, Garry C, Staples RC, Eversmeyer M, Fellers JP (2006) Transient expression and insertional mutagenesis of Puccinia triticina using biolistics. Funct Integr Genomics 6:250-260
36. Wolff AM, Arnau J (2002) Cloning of glyceraldehyde-3-phosphate dehydrogenase-encoding genes in Mucor circinelloides (syn. racemosus) and use of the gpd1 promoter for recombinant protein production. Fungal Genet Biol 35:21-29
37. Zhao X, Wu S, Hu C, Wang Q, Hua Y, Zhao ZK (2010) Lipid production from Jerusalem artichoke by Rhodosporidium toruloides Y4. J Ind Microbiol Biotechnol 37:581-585