The human anti-HIV antibodies 2F5, 2G12, and PG9 differ in their susceptibility to proteolytic degradation: Down-regulation of endogenous serine and cysteine proteinase activities could improve antibody production in plant-based expression platforms

Biotechnology Journal

Volumn 9, Issue 4, 2014, Pages 493-500

  Niemer, Melanie ^a   Mehofer, Ulrich ^a   Torres Acosta, Juan Antonio ^a   Verdianz, Maria ^a   Henkel, Theresa ^a   Loos, Andreas ^a   Strasser, Richard ^a   Maresch, Daniel ^a   Rademacher, Thomas ^b   Steinkellner, Herta ^a   Mach, Lukas ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Antibodies; Biopharmaceutical; Nicotiana; Protein stability; Proteinase

Indexed keywords

AMINO ACIDS; DEGRADATION; DISEASES; PLANTS (BOTANY);

BIOPHARMACEUTICAL; DIFFERENTIAL SUSCEPTIBILITY; NICOTIANA; NICOTIANA BENTHAMIANA; PLANT-BASED EXPRESSION; PROTEIN STABILITY; PROTEINASE; PROTEOLYTIC DEGRADATION;

ANTIBODIES;

ANTI HUMAN IMMUNODEFICIENCY VIRUS AGENT; CYSTEINE PROTEINASE; MONOCLONAL ANTIBODY 2F5; MONOCLONAL ANTIBODY 2G12; MONOCLONAL ANTIBODY PG9; POLYPEPTIDE; SERINE; UNCLASSIFIED DRUG; HUMAN IMMUNODEFICIENCY VIRUS ANTIBODY; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN; SERINE PROTEINASE;

AMINO TERMINAL SEQUENCE; ANTIBODY PRODUCTION; ANTIGEN BINDING; ANTIVIRAL SUSCEPTIBILITY; ARTICLE; DOWN REGULATION; ENZYME ACTIVITY; EXPRESSION VECTOR; FEMALE; HINGE REGION; IMMUNOBLOTTING; IN VITRO STUDY; NICOTIANA BENTHAMIANA; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PURIFICATION; ANIMAL; CHEMISTRY; CHO CELL LINE; CRICETULUS; GENETICS; HAMSTER; HUMAN; METABOLISM; PROTEIN STABILITY; TOBACCO; TRANSGENIC PLANT;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; CYSTEINE PROTEASES; DOWN-REGULATION; HIV ANTIBODIES; HUMANS; PLANTS, GENETICALLY MODIFIED; PROTEIN STABILITY; RECOMBINANT PROTEINS; SERINE PROTEASES; TOBACCO;

EID: 84897105776     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201300207     Document Type: Article

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