Analysis of post-translational modifications in recombinant monoclonal antibody IgG1 by reversed-phase liquid chromatography/mass spectrometry

Journal of Chromatography A

Volumn 1164, Issue 1-2, 2007, Pages 153-161

  Yan, Boxu ^a   Valliere Douglass, John ^a   Brady, Lowell ^a   Steen, Sean ^a   Han, Mei ^a   Pace, Danielle ^a   Elliott, Susan ^a   Yates, Zac ^a   Han, Yihong ^a   Balland, Alain ^a   Wang, Weichun ^a   Pettit, Dean ^a  

^a AMGEN INC   (United States)

Author keywords

Antibody; Cyclization; Deamidation; Glycosylation; Isomerization; LC TOF MS; Oxidation; Peptide mapping; RP HPLC

Indexed keywords

BIOPHARMACEUTICAL; PAPAIN CLEAVAGE; PYROGLUTAMIC ACID; SUBDOMAINS;

ACETONITRILE; ELECTROSPRAY IONIZATION; ISOMERIZATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; SEPARATION;

MONOCLONAL ANTIBODIES;

FC RECEPTOR; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G1 ANTIBODY; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; PAPAIN; PYROGLUTAMIC ACID; RECOMBINANT ANTIBODY;

ACCURACY; ANALYTIC METHOD; ANTIBODY DETECTION; ARTICLE; CYCLIZATION; DEAMINATION; ELECTROSPRAY MASS SPECTROMETRY; GLYCOSYLATION; ISOMERIZATION; MASS SPECTROMETRY; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; PROTEIN ISOLATION; PROTEIN MODIFICATION; PROTEIN PROCESSING; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE;

ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, LIQUID; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN G; OXIDATION-REDUCTION; PAPAIN; PEPTIDE MAPPING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRYPSIN;

EID: 34548040973     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2007.06.063     Document Type: Article

References (35)

1. Schrama D., Reisfeld R., and Becker J.C. Nature Rev. Durg Discov. 5 (2006) 147
2. In: Shen F.J., Kwong M.Y., Keck R.G., Harris R.J., and Marshak D.R. (Eds). Techniques in Protein Chemistry (1996), Academic Press, San Diego, CA 275
3. Houde D., Kauppinen P., Hatre R.M., and Lyubarskaya Y. J. Chromatogr. A 1053 (2004) 299
4. Chelius D., Jing K., Lueras A., Rehder D.S., Dillon T.M., Vizel A., Rajan R.S., Li T., Treuheit M.J., and Bondarenko P.V. Anal. Chem. 76 (2006) 2370
5. Rehder D.S., Dillon T.M., Pipes G.D., and Bondarenko P.V. J. Chromatogr. A 1102 (2006) 164
6. Moorhouse K.G., Nashabeh W., Deveney J., Bjork N.S., Mulkerrin M.G., and Ryskamp T. J. Pharm. Biomed. Anal. 16 (1997) 593
7. Harris R.J., Shire S.J., and Winter C. Drug Dev. Res. 61 (2004) 137
8. Verity C.K., Loukas A., McManus D.P., and Brindley P.J. Parasitology 122 (2001) 415
9. Berasain P., Carmona C., Frangione B., Dalton J.P., and Goni F. Exp. Parasitol. 94 (2000) 99
10. Schauenstein E., Dachs F., Reiter M., Gombotz H., and List W. Int. Arch. Allergy Immunol. 80 (1986) 174
11. Horejsi R., Kollenz G., Dachs F., Tillian H.M., Schauenstein K., Schauenstein E., and Steinschifter W. J. Biochem. Biophys. Methods 34 (1997) 227
12. Harris R.J., Kabakoff B., Macchi F.D., Shen F.J., Kwong M.Y., Andya J.D., Shire S.J., Bjork N., Totpal K., and Chen A.B. J. Chromatogr. B 752 (2001) 233
13. Huang L., Lu J., Wroblewski V.J., Beals J.M., and Riggin R.M. Anal. Chem. 77 (2006) 1432
14. Chelius D., Rehder D.S., and Bondarenko P.V. Anal. Chem. 77 (2005) 6004
15. Cournoyer J.J., Lin C., and O'Connor P.B. Anal. Chem. 78 (2006) 1264
16. Cacia J., Keck R., Presta L.G., and Frenz J. Biochemistry 35 (1996) 1897
17. Perkins M., Theiler R., Lunte S., and Jeschke M. Pharm. Res. 17 (2000) 1110
18. Zhang W., and Czupryn M.J. J. Pharm. Biomed. Anal. 30 (2003) 1479
19. Lam X.M., Yang J.Y., and Cleland J.L. J. Pharm. Sci. 86 (1997) 1250
20. Weitzhandler M., Farnan D., Horvath J., Rohrer J.S., Slingsby R.W., Avdalovic N., and Pohl C. J. Chromatogr. A 828 (1998) 365
21. Ma S., and Nashabeh W. Anal. Chem. 71 (1999) 5185
22. Hunt G., and Nashabeh W. Anal. Chem. 71 (1999) 2390
23. Wan H.Z., Kaneshiro S., Frenz J., and Cacia J. J. Chromatogr. A 913 (2001) 437
24. Dillon T.M., Bondarenko P.V., and Ricci M.S. J. Chromatogr. A 1053 (2004) 299
25. Zhang Z. Anal. Chem. 76 (2004) 3908
26. Zhang Z. Anal. Chem. 76 (2004) 6374
27. Lagerwerf F.M., Weert M., Heerma W., and Haverkamp J. Rapid Commun. Mass. Spectrom. 10 (1996) 1905
28. Jefferis R. Biotech Prog. 21 (2005) 11
29. Krokhin O.V., Antonovici M., Ens W., Wilkins J.A., and Standing K.G. Anal. Chem. 78 (2006) 6645
30. Hovorka S.W., and Schoneich C. J. Pharm. Sci. 90 (2001) 253
31. Slavica A., Dib I., and Nidetzky B. Appl. Environ. Microbiol. 71 (2005) 8061
32. Lu H.S., Fausset P.R., Narhi L.O., Horan T., Shinagawa K., Shimamoto G., and Boone T.C. Arch. Biochem. Biophys. 362 (1999) 1
33. Stadtman E.R., Moskovitz J., Berlett B.S., and Levine R.L. Mol. Cell Biochem. 234-235 (2002) 3
34. Moskovitz J., Poston J.M., Berlett B.S., Nosworhy N.J., Szczepanowski R., and Stadtman E.R. J. Biol. Chem. 275 (2000) 14167
35. Yu L., Vizel A., Huff M.B., Young M., Remmele R.L., and He B. J. Pharm. Biomed. Anal. 42 (2006) 455